Restriction map for
pFN21AB5490
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvaI AviII BalI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MamI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AciI 402 GCGG CCGC(-3/-1)
Alw44I 1082 GTGCAC G^TGCAC
ApaLI 1082 GTGCAC G^TGCAC
Asp700I 1276 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1114 CCTAGG C^CTAGG
BamHI 1197 GGATCC G^GATCC
BanI 1219 GGTGCC G^G[CT][GA]CC
BanII 1339 GGGCTC G[GA]GC[CT]^C
BciVI 416 GTATCC GTATCC(6/5)
BlnI 1114 CCTAGG C^CTAGG
BpmI 554 CTCCAG CTGGAG(16/14)
BsiYI 803 CCAGTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 803 CCAGTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 903 GCAGGT ACCTGC(4/8)
BsrDI 66 CATTGC GCAATG(2/0)
BssSI 1180 CTCGTG CACGAG(-5/-1)
BstAPI 664 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 774 CCAGAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1242 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 745 GAAGAG CTCTTC(1/4)
FauI 402 GCGGG CCCGC(4/6)
HgaI 341 GACGC GACGC(5/10)
HindIII 329 AAGCTT A^AGCTT
Ksp632I 745 GAAGAG CTCTTC(1/4)
MfeI 1382 CAATTG C^AATTG
MunI 1382 CAATTG C^AATTG
ScaI 531 AGTACT AGT^ACT
XmnI 1276 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflIII 55 ACATGT A^C[GA][CT]GT
1253 ACATGT
AhdI 1165 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1322 GACGGCGTGTC
AspEI 1165 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1322 GACGGCGTGTC
BglII 17 AGATCT A^GATCT
312 AGATCT
BsaJI 1114 CCTAGG C^C[ACGT][ACGT]GG
1193 CCAAGG
BseRI 930 GAGGAG GAGGAG(10/8)
933 GAGGAG
BsgI 32 GTGCAG GTGCAG(16/14)
574 GTGCAG
BsmFI 1250 GGGAC GGGAC(10/14)
1173 GTCCC
BsmI 1009 GCATTC GAATGC(1/-1)
1280 GCATTC
BspLU11I 55 ACATGT A^CATGT
1253 ACATGT
Eam1105I 1165 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1322 GACGGCGTGTC
EcoRI 135 GAATTC G^AATTC
1409 GAATTC
EcoT14I 1114 CCTAGG C^C[AT][AT]GG
1193 CCAAGG
PpuMI 48 AGGACCC [GA]G^G[AT]CC[CT]
509 AGGACCT
SspI 220 AATATT AAT^ATT
1064 AATATT
StyI 1114 CCTAGG C^C[AT][AT]GG
1193 CCAAGG
TaqI 192 TCGA T^CGA
1316 TCGA
Tsp45I 376 GTGAC ^GT[GC]AC
1183 GTGAC
TthHB8I 192 TCGA T^CGA
1316 TCGA
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII BbsI BbvI
BcnI BfaI BmyI BpuAI BsiHKAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI XhoII