(Circular) (Six-base) MAPSORT of: pFN21AB5490.seq Check: 7693 from: 1 to: 6187 pFN21AB5490 6187 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2634 4250 5634 5902 1632 Size: 710 292 1616 1384 268 1917 Fragments arranged by size: 1917 1616 1384 710 292 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 2060 3258 5086 6063 1203 Size: 857 1198 1828 977 1327 Fragments arranged by size: 1828 1327 1198 977 857 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 3175 3332 4916 3175 Size: 157 1584 4446 Fragments arranged by size: 4446 1584 157 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 2473 2587 2741 2984 3930 5502 2473 Size: 114 154 243 946 1572 3158 Fragments arranged by size: 3158 1572 946 243 154 114 ApaLI G'TGCA_C Cuts at: 3087 4244 5400 3087 Size: 1157 1156 3874 Fragments arranged by size: 3874 1157 1156 ApoI r'AATT_y Cuts at: 1493 2140 2461 2851 2878 3414 3438 3496 3722 Size: 647 321 390 27 536 24 58 226 Cuts at: 3722 3874 5983 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 647 536 390 321 226 152 58 27 24 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3512 1870 Size: 73 1569 4545 Fragments arranged by size: 4545 1569 73 AvrII C'CTAG_G Cuts at: 3119 3119 Size: 6187 BamHI G'GATC_C Cuts at: 3202 3517 3884 3202 Size: 315 367 5505 Fragments arranged by size: 5505 367 315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3224 3508 4963 618 Size: 325 217 627 1437 284 1455 1842 Fragments arranged by size: 1842 1455 1437 627 325 284 217 BanII G_rGCy'C Cuts at: 1391 3348 3506 1391 Size: 1957 158 4072 Fragments arranged by size: 4072 1957 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2640 3396 3446 928 Size: 971 741 756 50 3669 Fragments arranged by size: 3669 971 756 741 50 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 1903 Size: 34 2441 34 3678 Fragments arranged by size: 3678 2441 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2432 4079 5289 2432 Size: 1647 1210 3330 Fragments arranged by size: 3330 1647 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2022 2317 6182 2022 Size: 295 3865 2027 Fragments arranged by size: 3865 2027 295 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 3091 3229 4248 5404 1790 Size: 1301 138 1019 1156 2573 Fragments arranged by size: 2573 1301 1156 1019 138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2617 3918 4876 Size: 416 53 83 399 24 1200 1301 958 Cuts at: 4876 442 Size: 1753 Fragments arranged by size: 1753 1301 1200 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 4847 1239 Size: 1305 2303 2579 Fragments arranged by size: 2579 2303 1305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4850 882 Size: 617 3351 2219 Fragments arranged by size: 3351 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2730 2760 1178 Size: 30 165 30 1327 30 4605 Fragments arranged by size: 4605 1327 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2950 2953 1186 Size: 1764 3 4420 Fragments arranged by size: 4420 1764 3 BseYI C'CCAG_C Cuts at: 5390 5886 5390 Size: 496 5691 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2058 2600 1163 Size: 573 322 542 4750 Fragments arranged by size: 4750 573 542 322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2607 2722 3091 4248 4333 5404 2607 Size: 115 369 1157 85 1071 3390 Fragments arranged by size: 3390 1157 1071 369 115 85 BsmI GAATG_Cn' Cuts at: 3014 3285 3698 3791 3014 Size: 271 413 93 5410 Fragments arranged by size: 5410 413 271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2607 2722 3091 3229 3348 3506 4248 Size: 399 817 115 369 138 119 158 742 Cuts at: 4248 4333 5404 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 817 742 399 369 158 138 119 115 85 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2899 3542 844 Size: 2055 643 3489 Fragments arranged by size: 3489 2055 643 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 4682 4856 65 Size: 1183 822 2612 174 1396 Fragments arranged by size: 2612 1396 1183 822 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4831 5018 1700 Size: 110 3021 187 2869 Fragments arranged by size: 3021 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 3185 4247 5259 3185 Size: 1062 1012 4113 Fragments arranged by size: 4113 1062 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2675 794 Size: 1881 4306 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstXI CCAn_nnnn'nTGG Cuts at: 2786 2786 Size: 6187 BstYI r'GATC_y Cuts at: 1972 2022 2317 2625 3139 3202 3460 3517 3884 Size: 50 295 308 514 63 258 57 367 Cuts at: 3884 4269 4286 5738 5824 5836 6182 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 514 385 367 346 308 295 258 86 63 57 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5635 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 3703 4497 4517 3703 Size: 794 20 5373 Fragments arranged by size: 5373 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DraIII CAC_nnn'GTG Cuts at: 3252 3252 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3634 4523 6152 Size: 54 1425 50 275 18 1804 889 1629 Cuts at: 6152 8 Size: 43 Fragments arranged by size: 1804 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2745 4117 1353 Size: 1392 1372 3423 Fragments arranged by size: 3423 1392 1372 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2544 2634 4250 4847 5634 5902 Size: 393 710 202 90 1616 597 787 268 Cuts at: 5902 1239 Size: 1524 Fragments arranged by size: 1616 1524 787 710 597 393 268 202 90 EcoO109I rG'GnC_Cy Cuts at: 2054 2515 2584 2054 Size: 461 69 5657 Fragments arranged by size: 5657 461 69 EcoRI G'AATT_C Cuts at: 2140 3414 3496 3874 2140 Size: 1274 82 378 4453 Fragments arranged by size: 4453 1274 378 82 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2270 3063 3328 2270 Size: 793 265 5129 Fragments arranged by size: 5129 793 265 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 5334 1678 Size: 3656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2610 2642 3161 3193 3318 Size: 32 135 32 1038 32 519 32 125 Cuts at: 3318 3350 4828 4860 4902 4934 1373 Size: 32 1478 32 42 32 2626 Fragments arranged by size: 2626 1478 1038 519 135 125 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 2334 748 Size: 1586 4601 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3387 3786 3387 Size: 399 5788 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2029 2303 2502 5301 5485 1305 Size: 724 274 199 2799 184 2007 Fragments arranged by size: 2799 2007 724 274 199 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4365 Fragments arranged by size: 4365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2918 3102 3153 4145 4504 4663 518 Size: 648 1752 184 51 992 359 159 2042 Fragments arranged by size: 2042 1752 992 648 359 184 159 51 MspA1I CmG'CkG Cuts at: 1606 3603 4280 5428 5673 1606 Size: 1997 677 1148 245 2120 Fragments arranged by size: 2120 1997 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5635 NdeI CA'TA_TG Cuts at: 387 2079 3208 3421 387 Size: 1692 1129 213 3153 Fragments arranged by size: 3153 1692 1129 213 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2064 3219 3262 3545 5090 1132 Size: 97 835 1155 43 283 1545 2229 Fragments arranged by size: 2229 1545 1155 835 283 97 43 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 2060 3258 5086 2060 Size: 1198 1828 3161 Fragments arranged by size: 3161 1828 1198 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PfoI T'CCnGG_A Cuts at: 2951 3336 2951 Size: 385 5802 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2054 2515 2054 Size: 461 5726 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 3539 830 Size: 2709 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 2538 4435 6147 2538 Size: 1897 1712 2578 Fragments arranged by size: 2578 1897 1712 SfcI C'TryA_G Cuts at: 826 1045 3535 4670 5351 5542 826 Size: 219 2490 1135 681 191 1471 Fragments arranged by size: 2490 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 2227 3071 4111 5 Size: 47 2175 844 1040 2081 Fragments arranged by size: 2175 2081 1040 844 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 3119 3198 3626 513 Size: 552 2054 79 428 3074 Fragments arranged by size: 3074 2054 552 428 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4214 4231 4384 4569 5014 325 Size: 1255 2634 17 153 185 445 1498 Fragments arranged by size: 2634 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2536 4433 6145 Size: 274 80 33 51 1443 559 1897 1712 Cuts at: 6145 96 Size: 138 Fragments arranged by size: 1897 1712 1443 559 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6147 6179 6147 Size: 32 6155 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 3285 4316 3285 Size: 1031 5156 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI