(Circular) (Six-base) MAPSORT of: pFN21AB5464.seq Check: 3431 from: 1 to: 5503 pFN21AB5464 5503 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2192 2192 Size: 5503 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3313 278 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2846 1937 Size: 909 4594 Acc65I G'GTAC_C Cuts at: 2137 2824 2137 Size: 687 4816 AclI AA'CG_TT Cuts at: 1563 3630 4003 1563 Size: 2067 373 3063 Fragments arranged by size: 3063 2067 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3566 4950 5218 1632 Size: 1934 1384 268 1917 Fragments arranged by size: 1934 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5306 AflIII A'CryG_T Cuts at: 1203 2259 4402 5379 1203 Size: 1056 2143 977 1327 Fragments arranged by size: 2143 1327 1056 977 AgeI A'CCGG_T Cuts at: 4334 4334 Size: 5503 AhdI GACnn_n'nnGTC Cuts at: 4232 4232 Size: 5503 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5503 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5503 AlwNI CAG_nnn'CTG Cuts at: 3246 4818 3246 Size: 1572 3931 ApaLI G'TGCA_C Cuts at: 3560 4716 3560 Size: 1156 4347 ApoI r'AATT_y Cuts at: 1493 2387 2774 2812 3038 3190 5299 1493 Size: 894 387 38 226 152 2109 1697 Fragments arranged by size: 2109 1697 894 387 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 4057 160 Size: 872 3025 1606 Fragments arranged by size: 3025 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 AvaI C'yCGr_G Cuts at: 1870 1943 2828 1870 Size: 73 885 4545 Fragments arranged by size: 4545 885 73 BamHI G'GATC_C Cuts at: 2833 3200 2833 Size: 367 5136 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2824 4279 618 Size: 325 217 627 350 687 1455 1842 Fragments arranged by size: 1842 1455 687 627 350 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2313 2822 1391 Size: 673 249 509 4072 Fragments arranged by size: 4072 673 509 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4532 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2731 2765 3694 3728 1903 Size: 34 794 34 929 34 3678 Fragments arranged by size: 3678 929 794 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3395 4605 3395 Size: 1210 4293 BclI T'GATC_A Cuts at: 1548 2641 1548 Size: 1093 4410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4114 136 Size: 107 122 71 631 605 2442 1525 Fragments arranged by size: 2442 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2476 5498 2476 Size: 3022 2481 BlpI GC'TnA_GC Cuts at: 2920 2920 Size: 5503 Bme1580I G_kGCm'C Cuts at: 1790 3564 4720 1790 Size: 1774 1156 2573 Fragments arranged by size: 2573 1774 1156 BmgBI CAC'GTC Cuts at: 1318 2260 1318 Size: 942 4561 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3234 4192 442 Size: 416 53 83 399 24 1817 958 1753 Fragments arranged by size: 1817 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5503 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2567 4163 1239 Size: 1328 1596 2579 Fragments arranged by size: 2579 1596 1328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3628 4493 4791 3628 Size: 865 298 4340 Fragments arranged by size: 4340 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4166 882 Size: 617 2667 2219 Fragments arranged by size: 2667 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3294 493 Size: 711 2090 2702 Fragments arranged by size: 2702 2090 711 BsaBI GATnn'nnATC Cuts at: 2424 5497 2424 Size: 3073 2430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 3310 3692 4327 Size: 53 83 186 936 567 1210 382 635 Cuts at: 4327 275 Size: 1451 Fragments arranged by size: 1451 1210 936 635 567 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3936 4334 4608 4755 5166 3936 Size: 398 274 147 411 4273 Fragments arranged by size: 4273 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5278 Fragments arranged by size: 5278 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2232 1186 Size: 904 142 4457 Fragments arranged by size: 4457 904 142 BseYI C'CCAG_C Cuts at: 2326 4706 5202 2326 Size: 2380 496 2627 Fragments arranged by size: 2627 2380 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4930 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2953 3714 4742 1540 Size: 275 186 952 761 1028 2301 Fragments arranged by size: 2301 1028 952 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2610 3564 3649 4720 2610 Size: 954 85 1071 3393 Fragments arranged by size: 3393 1071 954 85 BsmI GAATG_Cn' Cuts at: 3014 3107 3014 Size: 93 5410 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2313 2610 2822 3564 3649 4720 Size: 399 274 249 297 212 742 85 1071 Cuts at: 4720 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 297 274 249 212 85 BspEI T'CCGG_A Cuts at: 5166 5166 Size: 5503 BspHI T'CATG_A Cuts at: 2443 2602 2674 3392 5122 2443 Size: 159 72 718 1730 2824 Fragments arranged by size: 2824 1730 718 159 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2192 2858 844 Size: 1348 666 3489 Fragments arranged by size: 3489 1348 666 BsrBI CCG'CTC Cuts at: 1394 1823 2030 2070 3390 1394 Size: 429 207 40 1320 3507 Fragments arranged by size: 3507 1320 429 207 40 BsrDI GCAATG_nn' Cuts at: 65 1248 3998 4172 65 Size: 1183 2750 174 1396 Fragments arranged by size: 2750 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4147 4334 1700 Size: 110 2337 187 2869 Fragments arranged by size: 2869 2337 187 110 BsrGI T'GTAC_A Cuts at: 96 2229 96 Size: 2133 3370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5503 BssSI C'ACGA_G Cuts at: 3563 4575 3563 Size: 1012 4491 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5503 BstBI TT'CG_AA Cuts at: 4307 4307 Size: 5503 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5503 BstYI r'GATC_y Cuts at: 1972 2476 2833 3200 3585 3602 5054 5140 5152 Size: 504 357 367 385 17 1452 86 12 Cuts at: 5152 5498 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 504 385 367 357 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2781 513 Size: 552 939 777 3235 Fragments arranged by size: 3235 939 777 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4764 Fragments arranged by size: 4764 683 56 BtsI GCAGTG_nn' Cuts at: 3019 3813 3833 3019 Size: 794 20 4689 Fragments arranged by size: 4689 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5468 DraI TTT'AAA Cuts at: 2807 2807 Size: 5503 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4510 809 Size: 507 3194 1802 Fragments arranged by size: 3194 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2146 2950 3839 Size: 54 1425 50 275 18 316 804 889 Cuts at: 3839 5468 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 804 316 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2950 1537 Size: 275 1138 4090 Fragments arranged by size: 4090 1138 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2149 3433 1353 Size: 796 1284 3423 Fragments arranged by size: 3423 1284 796 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4090 4464 4610 387 Size: 1064 138 2501 374 146 1280 Fragments arranged by size: 2501 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2567 3566 4163 4950 5218 1239 Size: 393 935 999 597 787 268 1524 Fragments arranged by size: 1524 999 935 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2122 2122 Size: 5503 EcoRI G'AATT_C Cuts at: 2812 3190 2812 Size: 378 5125 FspI TGC'GCA Cuts at: 4009 4009 Size: 5503 HaeII r_GCGC'y Cuts at: 1678 2084 4650 1678 Size: 406 2566 2531 Fragments arranged by size: 2566 2531 406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373 Size: 32 135 32 2572 32 42 32 2626 Fragments arranged by size: 2626 2572 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2847 3093 669 Size: 988 281 909 246 3079 Fragments arranged by size: 3079 988 909 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5503 HpaI GTT'AAC Cuts at: 3093 3093 Size: 5503 KpnI G_GTAC'C Cuts at: 2141 2828 2141 Size: 687 4816 MfeI C'AATT_G Cuts at: 2346 2743 3102 2346 Size: 397 359 4747 Fragments arranged by size: 4747 397 359 MluI A'CGCG_T Cuts at: 5379 5379 Size: 5503 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4617 4801 1305 Size: 3312 184 2007 Fragments arranged by size: 3312 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2148 10 Size: 54 1425 343 316 3365 Fragments arranged by size: 3365 1425 343 316 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 3461 3820 3979 518 Size: 648 1359 936 359 159 2042 Fragments arranged by size: 2042 1359 936 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2330 2919 3596 4744 4989 1606 Size: 724 589 677 1148 245 2120 Fragments arranged by size: 2120 1148 724 677 589 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4012 Fragments arranged by size: 4012 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5503 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5503 NotI GC'GGCC_GC Cuts at: 2950 2950 Size: 5503 NspI r_CATG'y Cuts at: 1132 1229 2176 2861 4406 1132 Size: 97 947 685 1545 2229 Fragments arranged by size: 2229 1545 947 685 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5503 PciI A'CATG_T Cuts at: 4402 4402 Size: 5503 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5503 PmeI GTTT'AAAC Cuts at: 2807 2807 Size: 5503 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3572 3604 5085 5117 3572 Size: 32 1481 32 3958 Fragments arranged by size: 3958 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 2122 Size: 5503 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5503 PsiI TTA'TAA Cuts at: 3073 3073 Size: 5503 PstI C_TGCA'G Cuts at: 830 2320 2855 830 Size: 1490 535 3478 Fragments arranged by size: 3478 1490 535 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5503 PvuII CAG'CTG Cuts at: 2330 2330 Size: 5503 SalI G'TCGA_C Cuts at: 1936 2845 1936 Size: 909 4594 SapI GCTCTTCn'nnn_ Cuts at: 2149 2149 Size: 5503 SbfI CC_TGCA'GG Cuts at: 2855 2855 Size: 5503 ScaI AGT'ACT Cuts at: 3751 5463 3751 Size: 1712 3791 SfcI C'TryA_G Cuts at: 826 1045 2316 2851 3986 4667 4858 826 Size: 219 1271 535 1135 681 191 1471 Fragments arranged by size: 1471 1271 1135 681 535 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5503 SmaI CCC'GGG Cuts at: 1872 2830 1872 Size: 958 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3607 4508 4770 820 Size: 197 926 1664 901 262 1553 Fragments arranged by size: 1664 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3294 493 Size: 2801 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5503 SphI G_CATG'C Cuts at: 1132 2861 1132 Size: 1729 3774 SspI AAT'ATT Cuts at: 5 52 2237 2359 3427 5 Size: 47 2185 122 1068 2081 Fragments arranged by size: 2185 2081 1068 122 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5503 StyI C'CwwG_G Cuts at: 513 1065 2004 2942 513 Size: 552 939 938 3074 Fragments arranged by size: 3074 939 938 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2548 3530 3547 3700 3885 4330 325 Size: 1255 968 982 17 153 185 445 1498 Fragments arranged by size: 1498 1255 982 968 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 2262 2280 Size: 274 80 33 51 1443 252 33 18 Cuts at: 2280 3749 5461 96 Size: 1469 1712 138 Fragments arranged by size: 1712 1469 1443 274 252 138 80 51 33 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5463 5495 5463 Size: 32 5471 Tth111I GACn'n_nGTC Cuts at: 1316 2102 2670 1316 Size: 786 568 4149 Fragments arranged by size: 4149 786 568 XbaI T'CTAG_A Cuts at: 2839 2839 Size: 5503 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2542 2542 Size: 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5503 XmaI C'CCGG_G Cuts at: 1870 2828 1870 Size: 958 4545 XmnI GAAnn'nnTTC Cuts at: 3632 3632 Size: 5503 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3311 276 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI