Restriction map for
pFN21AB5289
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AvrII BbeI BbrPI
BbvCI BciVI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MaeI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 358 GGTACC G^GTACC
AocI 739 CCTAAGG CC^T[ACGT]AGG
Asp700I 671 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 358 GGTACC G^GTACC
AspI 379 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 443 TGCGCA TGC^GCA
BalI 254 TGGCCA TGG^CCA
BamHI 129 GGATCC G^GATCC
BbsI 915 GAAGAC GAAGAC(2/6)
BcgI 224 CGACCAACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 335 TGATCA T^GATCA
BglI 262 GCCCTCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 771 AGATCT A^GATCT
BpmI 666 CTCCAG CTGGAG(16/14)
BpuAI 915 GAAGAC GAAGAC(2/6)
BsaBI 766 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 805 ACCGGA [AT]^CCGG[AT]
BsmI 611 GCATTC GAATGC(1/-1)
BspHI 894 TCATGA T^CATGA
BspMI 527 ACCTGC ACCTGC(4/8)
BsrBI 284 GAGCGG CCGCTC(-3/-3)
BsrI 545 ACTGG ACTGG(1/-1)
BstXI 455 CCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 739 CCTAAGG CC^T[ACGT]AGG
EaeI 254 TGGCCA [CT]^GGCC[GA]
Eco81I 739 CCTAAGG CC^T[ACGT]AGG
EcoT14I 354 CCATGG C^C[AT][AT]GG
FbaI 335 TGATCA T^GATCA
FspI 443 TGCGCA TGC^GCA
HaeII 111 GGCGCT [GA]GCGC^[CT]
KpnI 358 GGTACC GGTAC^C
MaeII 230 ACGT A^CGT
MamI 766 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 254 TGGCCA TGG^CCA
MscI 254 TGGCCA TGG^CCA
MseI 1169 TTAA T^TAA
NcoI 354 CCATGG C^CATGG
NdeI 482 CATATG CA^TATG
PaeR7I 682 CTCGAG C^TCGAG
PflFI 379 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 719 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 894 TCATGA T^CATGA
SacI 370 GAGCTC GAGCT^C
SbfI 984 CCTGCAGG CCTGCA^GG
SfiI 261 GGCCCTCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 984 CCTGCAGG CCTGCA^GG
StyI 354 CCATGG C^C[AT][AT]GG
TaqI 683 TCGA T^CGA
Tru9I 1169 TTAA T^TAA
Tth111I 379 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 683 TCGA T^CGA
XhoI 682 CTCGAG C^TCGAG
XmnI 671 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 593 AAATTC [GA]^AATT[CT]
852 GAATTT
ApaI 1211 GGGCCC GGGCC^C
1248 GGGCCC
ApoI 593 AAATTC [GA]^AATT[CT]
852 GAATTT
AspHI 232 GTGCTC G[AT]GC[AT]^C
370 GAGCTC
AvaI 40 CTCGGG C^[CT]CG[GA]G
682 CTCGAG
BanI 358 GGTACC G^G[CT][GA]CC
465 GGCACC
BsgI 550 GTGCAG GTGCAG(16/14)
922 GTGCAG
BsiHKAI 232 GTGCTC G[AT]GC[AT]^C
370 GAGCTC
BsmAI 172 GTCTC GTCTC(1/5)
240 GTCTC
BsoBI 40 CTCGGG C^[CT]CG[GA]G
682 CTCGAG
BstYI 129 GGATCC [GA]^GATC[CT]
771 AGATCT
DsaI 257 CCACGG C^C[GA][CT]GG
354 CCATGG
EarI 1219 CTCTTC CTCTTC(1/4)
703 GAAGAG
FauI 1152 CCCGC CCCGC(4/6)
1105 GCGGG
Ksp632I 1219 CTCTTC CTCTTC(1/4)
703 GAAGAG
MflI 129 GGATCC [GA]^GATC[CT]
771 AGATCT
MwoI 262 GCCCTCTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
512 GCAACCCTCGC
PstI 985 CTGCAG CTGCA^G
1129 CTGCAG
SfcI 985 CTGCAG C^T[GA][CT]AG
1129 CTGCAG
SmlI 406 CTCAAG C^T[CT][GA]AG
682 CTCGAG
Tsp509I 594 AATT ^AATT
853 AATT
XhoII 129 GGATCC [GA]^GATC[CT]
771 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI TfiI
TseI Tsp45I TspRI