(Circular) (Six-base) MAPSORT of: pFN21AB5081.seq Check: 1530 from: 1 to: 6328 pFN21AB5081 6328 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3354 3393 3671 1937 Size: 1417 39 278 4594 Fragments arranged by size: 4594 1417 278 39 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 4455 4828 1563 Size: 2892 373 3063 Fragments arranged by size: 3063 2892 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2775 4391 5775 6043 1632 Size: 1143 1616 1384 268 1917 Fragments arranged by size: 1917 1616 1384 1143 268 AfeI AGC'GCT Cuts at: 2258 2258 Size: 6328 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6131 AflIII A'CryG_T Cuts at: 1203 5227 6204 1203 Size: 4024 977 1327 Fragments arranged by size: 4024 1327 977 AgeI A'CCGG_T Cuts at: 3396 5159 3396 Size: 1763 4565 AhdI GACnn_n'nnGTC Cuts at: 5057 5057 Size: 6328 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2558 1184 Size: 1374 4954 AlwNI CAG_nnn'CTG Cuts at: 3491 4071 5643 3491 Size: 580 1572 4176 Fragments arranged by size: 4176 1572 580 ApaI G_GGCC'C Cuts at: 2252 3585 2252 Size: 1333 4995 ApaLI G'TGCA_C Cuts at: 4385 5541 4385 Size: 1156 5172 ApoI r'AATT_y Cuts at: 1493 3637 3863 4015 6124 1493 Size: 2144 226 152 2109 1697 Fragments arranged by size: 2144 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2127 2278 3653 1870 Size: 73 79 105 151 1375 4545 Fragments arranged by size: 4545 1375 151 105 79 73 BamHI G'GATC_C Cuts at: 3526 3658 4025 3526 Size: 132 367 5829 Fragments arranged by size: 5829 367 132 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2348 2387 2471 2662 3149 Size: 325 217 627 561 39 84 191 487 Cuts at: 3149 3170 3649 5104 618 Size: 21 479 1455 1842 Fragments arranged by size: 1842 1455 627 561 487 479 325 217 191 84 39 21 BanII G_rGCy'C Cuts at: 1391 2040 2055 2252 2958 3585 3647 1391 Size: 649 15 197 706 627 62 4072 Fragments arranged by size: 4072 706 649 627 197 62 15 BbeI G_GCGC'C Cuts at: 2352 2391 2475 3153 2352 Size: 39 84 678 5527 Fragments arranged by size: 5527 678 84 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2266 928 Size: 971 367 4990 Fragments arranged by size: 4990 971 367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2323 2357 4519 4553 1903 Size: 34 386 34 2162 34 3678 Fragments arranged by size: 3678 2162 386 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 2310 1548 Size: 762 5566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2192 2201 Size: 107 122 71 631 605 337 183 9 Cuts at: 2201 2385 2671 4939 136 Size: 184 286 2268 1525 Fragments arranged by size: 2268 1525 631 605 337 286 184 183 122 107 71 9 BglII A'GATC_T Cuts at: 6323 6323 Size: 6328 BlpI GC'TnA_GC Cuts at: 3215 3745 3215 Size: 530 5798 Bme1580I G_kGCm'C Cuts at: 1790 2252 2667 3585 4389 5545 1790 Size: 462 415 918 804 1156 2573 Fragments arranged by size: 2573 1156 918 804 462 415 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2222 4059 5017 Size: 416 53 83 399 24 805 1837 958 Cuts at: 5017 442 Size: 1753 Fragments arranged by size: 1837 1753 958 805 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3329 3329 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3324 4988 1239 Size: 2085 1664 2579 Fragments arranged by size: 2579 2085 1664 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2281 4453 5318 5616 2281 Size: 2172 865 298 2993 Fragments arranged by size: 2993 2172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2818 4991 882 Size: 617 1319 2173 2219 Fragments arranged by size: 2219 2173 1319 617 BsaAI yAC'GTr Cuts at: 493 1204 3460 4119 493 Size: 711 2256 659 2702 Fragments arranged by size: 2702 2256 711 659 BsaBI GATnn'nnATC Cuts at: 2312 2693 6322 2312 Size: 381 3629 2318 Fragments arranged by size: 3629 2318 381 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 2388 2429 2472 Size: 53 83 186 936 816 39 41 43 Cuts at: 2472 3029 3150 4135 4517 5152 275 Size: 557 121 985 382 635 1451 Fragments arranged by size: 1451 985 936 816 635 557 382 186 121 83 53 43 41 39 BsaWI w'CCGG_w Cuts at: 2645 3396 4761 5159 5433 5580 5991 2645 Size: 751 1365 398 274 147 411 2982 Fragments arranged by size: 2982 1365 751 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3347 3377 1178 Size: 30 165 30 1944 30 4129 Fragments arranged by size: 4129 1944 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2010 3113 3437 3554 1186 Size: 824 1103 324 117 3960 Fragments arranged by size: 3960 1103 824 324 117 BseYI C'CCAG_C Cuts at: 2206 2531 2746 2935 5531 6027 2206 Size: 325 215 189 2596 496 2507 Fragments arranged by size: 2596 2507 496 325 215 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2353 1163 Size: 573 617 5138 Fragments arranged by size: 5138 617 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2380 3354 3397 3603 3778 4539 Size: 275 186 379 974 43 206 175 761 Cuts at: 4539 5567 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 974 761 379 275 206 186 175 43 BsiHKAI G_wGCw'C Cuts at: 2547 4389 4474 5545 2547 Size: 1842 85 1071 3330 Fragments arranged by size: 3330 1842 1071 85 BsiWI C'GTAC_G Cuts at: 3467 3467 Size: 6328 BsmI GAATG_Cn' Cuts at: 3839 3932 3839 Size: 93 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2055 2252 2547 2667 2958 3585 Size: 399 250 15 197 295 120 291 627 Cuts at: 3585 3647 4389 4474 5545 1391 Size: 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 627 399 295 291 250 197 120 85 62 15 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 4217 5947 4217 Size: 1730 4598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 3683 844 Size: 1325 1514 3489 Fragments arranged by size: 3489 1514 1325 BsrBI CCG'CTC Cuts at: 1394 1823 4215 1394 Size: 429 2392 3507 Fragments arranged by size: 3507 2392 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3204 4823 4997 65 Size: 1183 1956 1619 174 1396 Fragments arranged by size: 1956 1619 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2195 3396 4972 5159 1700 Size: 110 385 1201 1576 187 2869 Fragments arranged by size: 2869 1576 1201 385 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6328 BssHII G'CGCG_C Cuts at: 1925 2370 1925 Size: 445 5883 BssSI C'ACGA_G Cuts at: 4388 5400 4388 Size: 1012 5316 BstAPI GCAn_nnn'nTGC Cuts at: 794 3593 794 Size: 2799 3529 BstBI TT'CG_AA Cuts at: 5132 5132 Size: 6328 BstEII G'GTnAC_C Cuts at: 1184 2413 1184 Size: 1229 5099 BstXI CCAn_nnnn'nTGG Cuts at: 2425 2809 2425 Size: 384 5944 BstYI r'GATC_y Cuts at: 1972 2560 3526 3658 4025 4410 4427 5879 5965 Size: 588 966 132 367 385 17 1452 86 Cuts at: 5965 5977 6323 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 966 588 385 367 346 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2187 2499 2508 2667 2802 2950 Size: 552 939 183 312 9 159 135 148 Cuts at: 2950 3597 513 Size: 647 3244 Fragments arranged by size: 3244 939 647 552 312 183 159 148 135 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 2521 3039 3204 3844 4638 4658 2521 Size: 518 165 640 794 20 4191 Fragments arranged by size: 4191 794 640 518 165 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3028 3063 529 Size: 35 2464 35 3794 Fragments arranged by size: 3794 2464 35 35 DraI TTT'AAA Cuts at: 3632 3632 Size: 6328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3389 5335 809 Size: 507 2073 1946 1802 Fragments arranged by size: 2073 1946 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2184 2193 Size: 54 1425 50 275 18 180 174 9 Cuts at: 2193 2377 3036 3192 3264 3321 3775 4664 6293 Size: 184 659 156 72 57 454 889 1629 Cuts at: 6293 8 Size: 43 Fragments arranged by size: 1629 1425 889 659 454 275 184 180 174 156 72 57 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2377 3775 1537 Size: 275 565 1398 4090 Fragments arranged by size: 4090 1398 565 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2625 2990 3061 3185 3503 4258 1353 Size: 1272 365 71 124 318 755 3423 Fragments arranged by size: 3423 1272 755 365 318 124 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 2470 3055 4915 5289 5435 Size: 1064 138 474 407 585 1860 374 146 Cuts at: 5435 387 Size: 1280 Fragments arranged by size: 1860 1280 1064 585 474 407 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2775 3324 4391 4988 5775 6043 1239 Size: 393 1143 549 1067 597 787 268 1524 Fragments arranged by size: 1524 1143 1067 787 597 549 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3303 3303 Size: 6328 EcoO109I rG'GnC_Cy Cuts at: 2068 2750 3051 3581 2068 Size: 682 301 530 4815 Fragments arranged by size: 4815 682 530 301 EcoRI G'AATT_C Cuts at: 3637 4015 3637 Size: 378 5950 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 2078 2207 2260 2268 2352 2391 2399 2438 Size: 400 129 53 8 84 39 8 39 Cuts at: 2438 2456 2475 2738 3153 5475 1678 Size: 18 19 263 415 2322 2531 Fragments arranged by size: 2531 2322 415 400 263 129 84 53 39 39 19 18 8 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3347 3379 3602 3634 4969 Size: 32 135 32 1775 32 223 32 1335 Cuts at: 4969 5001 5043 5075 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1775 1335 223 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3160 3343 3355 3394 3672 3918 Size: 988 281 1222 183 12 39 278 246 Cuts at: 3918 669 Size: 3079 Fragments arranged by size: 3079 1222 988 281 278 246 183 39 12 HindIII A'AGCT_T Cuts at: 748 748 Size: 6328 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KasI G'GCGC_C Cuts at: 2348 2387 2471 3149 2348 Size: 39 84 678 5527 Fragments arranged by size: 5527 678 84 39 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2610 5442 5626 1305 Size: 1305 2832 184 2007 Fragments arranged by size: 2832 2007 1305 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3038 3323 10 Size: 54 1425 343 1206 285 3015 Fragments arranged by size: 3015 1425 1206 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 4286 4645 4804 518 Size: 648 3120 359 159 2042 Fragments arranged by size: 3120 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2145 2323 2510 3034 3079 3290 3532 Size: 439 100 178 187 524 45 211 242 Cuts at: 3532 3744 4421 5569 5814 1606 Size: 212 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 524 439 245 242 212 211 187 178 100 45 NaeI GCC'GGC Cuts at: 2197 2197 Size: 6328 NarI GG'CG_CC Cuts at: 2349 2388 2472 3150 2349 Size: 39 84 678 5527 Fragments arranged by size: 5527 678 84 39 NcoI C'CATG_G Cuts at: 513 1065 2004 2802 2950 513 Size: 552 939 798 148 3891 Fragments arranged by size: 3891 939 798 552 148 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NgoMIV G'CCGG_C Cuts at: 2195 2195 Size: 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 3686 5231 1132 Size: 97 2457 1545 2229 Fragments arranged by size: 2457 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6328 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 3190 1108 Size: 2082 4246 PfoI T'CCnGG_A Cuts at: 2556 2816 2556 Size: 260 6068 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4397 4429 5910 5942 4397 Size: 32 1481 32 4783 Fragments arranged by size: 4783 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3051 3051 Size: 6328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3898 3898 Size: 6328 PspOMI G'GGCC_C Cuts at: 2248 3581 2248 Size: 1333 4995 PstI C_TGCA'G Cuts at: 830 2466 3680 830 Size: 1636 1214 3478 Fragments arranged by size: 3478 1636 1214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 SacII CC_GC'GG Cuts at: 2511 2511 Size: 6328 SalI G'TCGA_C Cuts at: 1936 3353 3392 3670 1936 Size: 1417 39 278 4594 Fragments arranged by size: 4594 1417 278 39 SapI GCTCTTCn'nnn_ Cuts at: 3185 3185 Size: 6328 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SexAI A'CCwGG_T Cuts at: 2133 2589 2133 Size: 456 5872 SfcI C'TryA_G Cuts at: 826 1045 2462 3676 4811 5492 5683 826 Size: 219 1417 1214 1135 681 191 1471 Fragments arranged by size: 1471 1417 1214 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2192 2192 Size: 6328 SfoI GGC'GCC Cuts at: 2350 2389 2473 3151 2350 Size: 39 84 678 5527 Fragments arranged by size: 5527 678 84 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6328 SmaI CCC'GGG Cuts at: 1872 2129 2280 3655 1872 Size: 257 151 1375 4545 Fragments arranged by size: 4545 1375 257 151 SmlI C'TyrA_G Cuts at: 820 1017 1943 2022 2260 4432 5333 5595 820 Size: 197 926 79 238 2172 901 262 1553 Fragments arranged by size: 2172 1553 926 901 262 238 197 79 SnaBI TAC'GTA Cuts at: 493 4119 493 Size: 3626 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6328 SphI G_CATG'C Cuts at: 1132 3686 1132 Size: 2554 3774 SspI AAT'ATT Cuts at: 5 52 4252 5 Size: 47 4200 2081 Fragments arranged by size: 4200 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6328 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2095 2802 2908 2950 3228 Size: 552 939 27 64 707 106 42 278 Cuts at: 3228 3767 513 Size: 539 3074 Fragments arranged by size: 3074 939 707 552 539 278 106 64 42 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4355 4372 4525 4710 5155 325 Size: 1255 2775 17 153 185 445 1498 Fragments arranged by size: 2775 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2916 4574 6286 Size: 274 80 33 51 1443 939 1658 1712 Cuts at: 6286 96 Size: 138 Fragments arranged by size: 1712 1658 1443 939 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2202 2234 2640 2672 6288 6320 2202 Size: 32 406 32 3616 32 2210 Fragments arranged by size: 3616 2210 406 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2806 3160 2068 Size: 738 354 5236 Fragments arranged by size: 5236 738 354 XhoI C'TCGA_G Cuts at: 1943 2022 1943 Size: 79 6249 XmaI C'CCGG_G Cuts at: 1870 2127 2278 3653 1870 Size: 257 151 1375 4545 Fragments arranged by size: 4545 1375 257 151 XmnI GAAnn'nnTTC Cuts at: 4457 4457 Size: 6328 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SrfI SwaI