(Circular) (Six-base) MAPSORT of: pFN21AB3587.seq Check: 6817 from: 1 to: 6169 pFN21AB3587 6169 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2519 3183 2519 Size: 664 5505 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 2439 3490 2439 Size: 1051 5118 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 4232 5616 5884 1632 Size: 697 1903 1384 268 1917 Fragments arranged by size: 1917 1903 1384 697 268 AflII C'TTAA_G Cuts at: 820 1017 2738 820 Size: 197 1721 4251 Fragments arranged by size: 4251 1721 197 AflIII A'CryG_T Cuts at: 1203 3069 5068 6045 1203 Size: 1866 1999 977 1327 Fragments arranged by size: 1999 1866 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AlwNI CAG_nnn'CTG Cuts at: 2297 3319 3912 5484 2297 Size: 1022 593 1572 2982 Fragments arranged by size: 2982 1572 1022 593 ApaI G_GGCC'C Cuts at: 2028 2958 3227 2028 Size: 930 269 4970 Fragments arranged by size: 4970 930 269 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 3104 3478 3704 3856 5965 1493 Size: 1611 374 226 152 2109 1697 Fragments arranged by size: 2109 1697 1611 374 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2134 3179 3494 1870 Size: 73 191 1045 315 4545 Fragments arranged by size: 4545 1045 315 191 73 BamHI G'GATC_C Cuts at: 2867 3499 3866 2867 Size: 632 367 5170 Fragments arranged by size: 5170 632 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2423 2439 3334 3490 4945 Size: 325 217 627 636 16 895 156 1455 Cuts at: 4945 618 Size: 1842 Fragments arranged by size: 1842 1455 895 636 627 325 217 156 16 BanII G_rGCy'C Cuts at: 1391 2028 2958 3150 3227 3488 1391 Size: 637 930 192 77 261 4072 Fragments arranged by size: 4072 930 637 261 192 77 BbeI G_GCGC'C Cuts at: 3338 3338 Size: 6169 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2185 928 Size: 971 286 4912 Fragments arranged by size: 4912 971 286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3088 3122 4360 4394 1903 Size: 34 50 34 1067 34 1238 34 3678 Fragments arranged by size: 3678 1238 1067 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 2578 2829 1548 Size: 1030 251 4888 Fragments arranged by size: 4888 1030 251 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3349 4780 136 Size: 107 122 71 631 605 1677 1431 1525 Fragments arranged by size: 1677 1525 1431 631 605 122 107 71 BglII A'GATC_T Cuts at: 6164 6164 Size: 6169 BlpI GC'TnA_GC Cuts at: 3458 3586 3458 Size: 128 6041 Bme1580I G_kGCm'C Cuts at: 1790 2028 2958 3200 3227 4230 5386 1790 Size: 238 930 242 27 1003 1156 2573 Fragments arranged by size: 2573 1156 1003 930 242 238 27 BmgBI CAC'GTC Cuts at: 1318 2698 3280 1318 Size: 1380 582 4207 Fragments arranged by size: 4207 1380 582 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3230 3900 4858 Size: 416 53 83 399 24 1813 670 958 Cuts at: 4858 442 Size: 1753 Fragments arranged by size: 1813 1753 958 670 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3355 3355 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2783 4829 1239 Size: 1544 2046 2579 Fragments arranged by size: 2579 2046 1544 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4294 5159 5457 4294 Size: 865 298 5006 Fragments arranged by size: 5006 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4832 882 Size: 617 3333 2219 Fragments arranged by size: 3333 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3397 3960 493 Size: 711 2193 563 2702 Fragments arranged by size: 2702 2193 711 563 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 3323 3335 3976 Size: 53 83 186 936 657 1133 12 641 Cuts at: 3976 4358 4993 275 Size: 382 635 1451 Fragments arranged by size: 1451 1133 936 657 641 635 382 186 83 53 12 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2054 2084 1178 Size: 30 165 30 651 30 5263 Fragments arranged by size: 5263 651 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 1186 Size: 873 5296 BseYI C'CCAG_C Cuts at: 3149 3400 5372 5868 3149 Size: 251 1972 496 3450 Fragments arranged by size: 3450 1972 496 251 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3155 1163 Size: 573 1419 4177 Fragments arranged by size: 4177 1419 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3619 4380 5408 1540 Size: 275 186 1618 761 1028 2301 Fragments arranged by size: 2301 1618 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3101 3303 4230 4315 5386 3101 Size: 202 927 85 1071 3884 Fragments arranged by size: 3884 1071 927 202 85 BsmI GAATG_Cn' Cuts at: 2681 3090 3680 3773 2681 Size: 409 590 93 5077 Fragments arranged by size: 5077 590 409 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2958 3101 3150 3200 3227 3303 Size: 399 238 930 143 49 50 27 76 Cuts at: 3303 3488 4230 4315 5386 1391 Size: 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 930 742 399 238 185 143 85 76 50 49 27 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 2169 4058 5788 2169 Size: 1889 1730 2550 Fragments arranged by size: 2550 1889 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2519 3183 3524 844 Size: 1675 664 341 3489 Fragments arranged by size: 3489 1675 664 341 BsrBI CCG'CTC Cuts at: 1394 1823 4056 1394 Size: 429 2233 3507 Fragments arranged by size: 3507 2233 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2542 4664 4838 65 Size: 1183 1294 2122 174 1396 Fragments arranged by size: 2122 1396 1294 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4813 5000 1700 Size: 110 3003 187 2869 Fragments arranged by size: 3003 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 2367 4229 5241 2367 Size: 1862 1012 3295 Fragments arranged by size: 3295 1862 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3204 794 Size: 2410 3759 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 3355 3355 Size: 6169 BstYI r'GATC_y Cuts at: 1972 2867 3499 3866 4251 4268 5720 5806 5818 Size: 895 632 367 385 17 1452 86 12 Cuts at: 5818 6164 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 895 632 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2076 3465 513 Size: 552 1011 1389 3217 Fragments arranged by size: 3217 1389 1011 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 3685 4479 4499 3685 Size: 794 20 5355 Fragments arranged by size: 5355 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 3065 3065 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2357 5176 809 Size: 507 1041 2819 1802 Fragments arranged by size: 2819 1802 1041 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 3408 3616 Size: 54 1425 50 275 18 649 929 208 Cuts at: 3616 4505 6134 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 929 889 649 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 2977 4099 1353 Size: 887 737 1122 3423 Fragments arranged by size: 3423 1122 887 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4756 5130 5276 387 Size: 1064 138 3167 374 146 1280 Fragments arranged by size: 3167 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2329 2783 4232 4829 5616 5884 1239 Size: 393 697 454 1449 597 787 268 1524 Fragments arranged by size: 1524 1449 787 697 597 454 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3260 3428 3260 Size: 168 6001 EcoO109I rG'GnC_Cy Cuts at: 2325 2325 Size: 6169 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 EcoRV GAT'ATC Cuts at: 2413 2413 Size: 6169 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 3338 5316 1678 Size: 1660 1978 2531 Fragments arranged by size: 2531 1978 1660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2976 3008 4810 4842 4884 Size: 32 135 32 1404 32 1802 32 42 Cuts at: 4884 4916 1373 Size: 32 2626 Fragments arranged by size: 2626 1802 1404 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3513 3759 669 Size: 988 281 1575 246 3079 Fragments arranged by size: 3079 1575 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KasI G'GCGC_C Cuts at: 3334 3334 Size: 6169 KpnI G_GTAC'C Cuts at: 2443 3494 2443 Size: 1051 5118 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2862 5283 5467 1305 Size: 1557 2421 184 2007 Fragments arranged by size: 2421 2007 1557 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2481 3410 10 Size: 54 1425 343 649 929 2769 Fragments arranged by size: 2769 1425 929 649 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3038 3395 4127 4486 4645 518 Size: 648 1872 357 732 359 159 2042 Fragments arranged by size: 2042 1872 732 648 359 357 159 MspA1I CmG'CkG Cuts at: 1606 3585 4262 5410 5655 1606 Size: 1979 677 1148 245 2120 Fragments arranged by size: 2120 1979 1148 677 245 NarI GG'CG_CC Cuts at: 3335 3335 Size: 6169 NcoI C'CATG_G Cuts at: 513 1065 2076 513 Size: 552 1011 4606 Fragments arranged by size: 4606 1011 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3073 3527 5072 1132 Size: 97 1844 454 1545 2229 Fragments arranged by size: 2229 1844 1545 454 97 PasI CC'CwG_GG Cuts at: 1348 2592 1348 Size: 1244 4925 PciI A'CATG_T Cuts at: 3069 5068 3069 Size: 1999 4170 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PfoI T'CCnGG_A Cuts at: 2760 2760 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PmlI CAC'GTG Cuts at: 3397 3397 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2325 2325 Size: 6169 PshAI GACnn'nnGTC Cuts at: 1613 2189 2207 1613 Size: 576 18 5575 Fragments arranged by size: 5575 576 18 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PspOMI G'GGCC_C Cuts at: 2024 2954 3223 2024 Size: 930 269 4970 Fragments arranged by size: 4970 930 269 PstI C_TGCA'G Cuts at: 830 2293 3124 3141 3521 830 Size: 1463 831 17 380 3478 Fragments arranged by size: 3478 1463 831 380 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SanDI GG'GwC_CC Cuts at: 2325 2325 Size: 6169 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SfcI C'TryA_G Cuts at: 826 1045 2289 2623 2908 3120 3137 3517 4652 Size: 219 1244 334 285 212 17 380 1135 Cuts at: 4652 5333 5524 826 Size: 681 191 1471 Fragments arranged by size: 1471 1244 1135 681 380 334 285 219 212 191 17 SfoI GGC'GCC Cuts at: 3336 3336 Size: 6169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 2136 3181 3496 1872 Size: 264 1045 315 4545 Fragments arranged by size: 4545 1045 315 264 SmlI C'TyrA_G Cuts at: 820 1017 1943 2738 4273 5174 5436 820 Size: 197 926 795 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 795 262 197 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6169 StyI C'CwwG_G Cuts at: 513 1065 2076 3608 513 Size: 552 1011 1532 3074 Fragments arranged by size: 3074 1532 1011 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 2708 4196 4213 4366 4551 4996 Size: 1255 495 633 1488 17 153 185 445 Cuts at: 4996 325 Size: 1498 Fragments arranged by size: 1498 1488 1255 633 495 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2043 4415 6127 Size: 274 80 33 51 1443 66 2372 1712 Cuts at: 6127 96 Size: 138 Fragments arranged by size: 2372 1712 1443 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2054 2086 6129 6161 2054 Size: 32 4043 32 2062 Fragments arranged by size: 4043 2062 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3217 3397 3217 Size: 180 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 2134 3179 3494 1870 Size: 264 1045 315 4545 Fragments arranged by size: 4545 1045 315 264 XmnI GAAnn'nnTTC Cuts at: 4298 4298 Size: 6169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI