(Circular) (Six-base) MAPSORT of: pFN21AB3574.seq Check: 946 from: 1 to: 6001 pFN21AB3574 6001 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2792 2792 Size: 6001 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2673 3344 1937 Size: 736 671 4594 Fragments arranged by size: 4594 736 671 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4064 5448 5716 1632 Size: 2432 1384 268 1917 Fragments arranged by size: 2432 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2060 3306 1184 Size: 876 1246 3879 Fragments arranged by size: 3879 1246 876 AlwNI CAG_nnn'CTG Cuts at: 2540 2908 3744 5316 2540 Size: 368 836 1572 3225 Fragments arranged by size: 3225 1572 836 368 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 2056 2213 2260 3310 3536 3688 5797 1493 Size: 563 157 47 1050 226 152 2109 1697 Fragments arranged by size: 2109 1697 1050 563 226 157 152 47 AseI AT'TA_AT Cuts at: 160 1032 2438 4555 160 Size: 872 1406 2117 1606 Fragments arranged by size: 2117 1606 1406 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3322 4777 618 Size: 325 217 627 1535 1455 1842 Fragments arranged by size: 1842 1535 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2652 2769 3320 1391 Size: 1261 117 551 4072 Fragments arranged by size: 4072 1261 551 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2067 2576 928 Size: 971 168 509 4353 Fragments arranged by size: 4353 971 509 168 BbvCI CC'TCA_GC Cuts at: 2496 2496 Size: 6001 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4192 4226 1903 Size: 34 2255 34 3678 Fragments arranged by size: 3678 2255 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2955 3893 5103 2955 Size: 938 1210 3853 Fragments arranged by size: 3853 1210 938 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BfrBI ATG'CAT Cuts at: 2402 3125 2402 Size: 723 5278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4612 136 Size: 107 122 71 631 605 2940 1525 Fragments arranged by size: 2940 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 2637 3418 2637 Size: 781 5220 Bme1580I G_kGCm'C Cuts at: 1790 4062 5218 1790 Size: 2272 1156 2573 Fragments arranged by size: 2573 2272 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 3732 4690 Size: 416 53 83 399 24 690 1625 958 Cuts at: 4690 442 Size: 1753 Fragments arranged by size: 1753 1625 958 690 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2562 4661 1239 Size: 1323 2099 2579 Fragments arranged by size: 2579 2099 1323 Bpu10I CC'TnA_GC Cuts at: 2220 2496 2220 Size: 276 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4126 4991 5289 4126 Size: 865 298 4838 Fragments arranged by size: 4838 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4664 882 Size: 617 3165 2219 Fragments arranged by size: 3165 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3019 3792 493 Size: 711 1815 773 2702 Fragments arranged by size: 2702 1815 773 711 BsaBI GATnn'nnATC Cuts at: 2624 5995 2624 Size: 3371 2630 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3808 4190 4825 275 Size: 53 83 186 936 2275 382 635 1451 Fragments arranged by size: 2275 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4434 4832 5106 5253 5664 4434 Size: 398 274 147 411 4771 Fragments arranged by size: 4771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5776 Fragments arranged by size: 5776 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 1186 Size: 896 5105 BseYI C'CCAG_C Cuts at: 2908 3027 5204 5700 2908 Size: 119 2177 496 3209 Fragments arranged by size: 3209 2177 496 119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2883 1163 Size: 573 1147 4281 Fragments arranged by size: 4281 1147 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2373 4062 4147 5218 2373 Size: 1689 85 1071 3156 Fragments arranged by size: 3156 1689 1071 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2373 2652 2769 3320 4062 4147 5218 Size: 399 583 279 117 551 742 85 1071 Cuts at: 5218 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 583 551 399 279 117 85 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2537 2792 3356 844 Size: 1693 255 564 3489 Fragments arranged by size: 3489 1693 564 255 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3273 4496 4670 65 Size: 1183 2025 1223 174 1396 Fragments arranged by size: 2025 1396 1223 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4645 4832 1700 Size: 110 2835 187 2869 Fragments arranged by size: 2869 2835 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 2372 4061 5073 2372 Size: 1689 1012 3300 Fragments arranged by size: 3300 1689 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6001 BstBI TT'CG_AA Cuts at: 2264 2799 4805 2264 Size: 535 2006 3460 Fragments arranged by size: 3460 2006 535 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstXI CCAn_nnnn'nTGG Cuts at: 2830 3031 2830 Size: 201 5800 BstYI r'GATC_y Cuts at: 1972 2424 3331 3698 4083 4100 5552 5638 5650 Size: 452 907 367 385 17 1452 86 12 Cuts at: 5650 5996 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 907 452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2250 513 Size: 552 939 246 4264 Fragments arranged by size: 4264 939 552 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 2025 2638 3517 4311 4331 2025 Size: 613 879 794 20 3695 Fragments arranged by size: 3695 879 794 613 20 ClaI AT'CG_AT Cuts at: 1554 3288 1554 Size: 1734 4267 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DraIII CAC_nnn'GTG Cuts at: 3010 3010 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2071 5008 809 Size: 507 755 2937 1802 Fragments arranged by size: 2937 1802 755 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3021 3111 3448 Size: 54 1425 50 275 18 1191 90 337 Cuts at: 3448 4337 5966 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1191 889 337 275 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3931 1353 Size: 2578 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2562 4064 4661 5448 5716 1239 Size: 393 930 1502 597 787 268 1524 Fragments arranged by size: 1524 1502 930 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2534 2534 Size: 6001 EcoO109I rG'GnC_Cy Cuts at: 2011 2995 2011 Size: 984 5017 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 5148 1678 Size: 3470 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 4642 4674 4716 Size: 32 135 32 505 32 2533 32 42 Cuts at: 4716 4748 1373 Size: 32 2626 Fragments arranged by size: 2626 2533 505 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2326 2793 3345 3591 669 Size: 988 281 388 467 552 246 3079 Fragments arranged by size: 3079 988 552 467 388 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 2793 3591 2793 Size: 798 5203 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3025 3279 5115 5299 1305 Size: 1720 254 1836 184 2007 Fragments arranged by size: 2007 1836 1720 254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3023 3113 10 Size: 54 1425 343 1191 90 2898 Fragments arranged by size: 2898 1425 1191 343 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 3288 3959 4318 4477 518 Size: 648 2122 671 359 159 2042 Fragments arranged by size: 2122 2042 671 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3031 3417 4094 5242 5487 1606 Size: 1425 386 677 1148 245 2120 Fragments arranged by size: 2120 1425 1148 677 386 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2250 513 Size: 552 939 246 4264 Fragments arranged by size: 4264 939 552 246 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NsiI A_TGCA'T Cuts at: 2404 3127 2404 Size: 723 5278 NspI r_CATG'y Cuts at: 1132 1229 2645 3359 4904 1132 Size: 97 1416 714 1545 2229 Fragments arranged by size: 2229 1545 1416 714 97 PacI TTA_AT'TAA Cuts at: 2442 2442 Size: 6001 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 2336 1108 Size: 1228 4773 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PmlI CAC'GTG Cuts at: 3019 3019 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PstI C_TGCA'G Cuts at: 830 3353 830 Size: 2523 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 3031 3031 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SfcI C'TryA_G Cuts at: 826 1045 2505 3349 4484 5165 5356 826 Size: 219 1460 844 1135 681 191 1471 Fragments arranged by size: 1471 1460 1135 844 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4105 5006 5268 820 Size: 197 926 2162 901 262 1553 Fragments arranged by size: 2162 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 2645 3359 1132 Size: 1513 714 3774 Fragments arranged by size: 3774 1513 714 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2004 2250 3440 513 Size: 552 939 246 1190 3074 Fragments arranged by size: 3074 1190 939 552 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3041 4028 4045 4198 4383 4828 325 Size: 1255 1461 987 17 153 185 445 1498 Fragments arranged by size: 1498 1461 1255 987 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4247 5959 96 Size: 274 80 33 51 1443 2270 1712 138 Fragments arranged by size: 2270 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2617 2649 5961 5993 2617 Size: 32 3312 32 2625 Fragments arranged by size: 3312 2625 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 3212 4130 3212 Size: 918 5083 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI