Restriction map for
pFN21AB3524
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HpaI KasI
KpnI KspI MluI MluNI MroI
MscI MwoI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 452 GACGTC GACGT^C
AccI 308 GTCTAC GT^[AC][GT]AC
AccII 97 CGCG CG^CG
AclI 748 AACGTT AA^CGTT
AcyI 452 GACGTC G[GA]^CG[CT]C
Asp700I 1087 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 79 CTCGGG C^[CT]CG[GA]G
AvaII 203 GGTCC G^G[AT]CC
BamHI 705 GGATCC G^GATCC
BbsI 1366 GAAGAC GAAGAC(2/6)
BcnI 206 CCGGG CC^[GC]GG
BpuAI 1366 GAAGAC GAAGAC(2/6)
BsaAI 1187 TACGTA [CT]AC^GT[GA]
BsaBI 946 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 452 GACGTC G[GA]^CG[CT]C
BsmAI 125 GAGAC GTCTC(1/5)
BsmBI 125 GAGACG CGTCTC(1/5)
BsoBI 79 CTCGGG C^[CT]CG[GA]G
BsrBI 362 GAGCGG CCGCTC(-3/-3)
BstNI 835 CCTGG CC^[AT]GG
BstUI 97 CGCG CG^CG
CfoI 96 GCGC GCG^C
Cfr13I 203 GGTCC G^G[ACGT]CC
DraI 741 TTTAAA TTT^AAA
EcoRI 716 GAATTC G^AATTC
EcoRII 835 CCTGG ^CC[AT]GG
EcoT14I 1097 CCAAGG C^C[AT][AT]GG
HapII 206 CCGG C^CGG
HhaI 96 GCGC GCG^C
Hin1I 452 GACGTC G[GA]^CG[CT]C
HinP1I 96 GCGC G^CGC
HincII 903 GTTGAC GT[CT]^[GA]AC
HindII 903 GTTGAC GT[CT]^[GA]AC
HpaII 206 CCGG C^CGG
MamI 946 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1319 CAATTG C^AATTG
MslI 580 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 206 CCGG C^CGG
MunI 1319 CAATTG C^AATTG
MvaI 835 CCTGG CC^[AT]GG
MvnI 97 CGCG CG^CG
NciI 206 CCGGG CC^[GC]GG
NheI 933 GCTAGC G^CTAGC
Psp1406I 748 AACGTT AA^CGTT
Sau96I 203 GGTCC G^G[ACGT]CC
SmlI 1351 CTTGAG C^T[CT][GA]AG
SnaBI 1187 TACGTA TAC^GTA
SspI 884 AATATT AAT^ATT
StyI 1097 CCAAGG C^C[AT][AT]GG
TspRI 1235 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 1087 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 509 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
830 CAGATCCTG
AocI 387 CCTGAGG CC^T[ACGT]AGG
709 CCTAAGG
BanII 76 GGGCTC G[GA]GC[CT]^C
841 GAGCCC
BmyI 76 GGGCTC G[AGT]GC[ACT]^C
841 GAGCCC
BpmI 91 CTGGAG CTGGAG(16/14)
680 CTGGAG
BsaJI 206 CCGGGG C^C[ACGT][ACGT]GG
1097 CCAAGG
BsiYI 110 CCGCTGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
955 CCCTATAGGGG
BslI 110 CCGCTGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
955 CCCTATAGGGG
Bsp1286I 76 GGGCTC G[AGT]GC[ACT]^C
841 GAGCCC
BspHI 399 TCATGA T^CATGA
543 TCATGA
BssKI 206 CCGGG ^CC[ACGT]GG
835 CCTGG
Bsu36I 387 CCTGAGG CC^T[ACGT]AGG
709 CCTAAGG
Cac8I 839 GCGAGC GC[ACGT]^[ACGT]GC
933 GCTAGC
EaeI 2 TGGCCG [CT]^GGCC[GA]
365 CGGCCA
Eco81I 387 CCTGAGG CC^T[ACGT]AGG
709 CCTAAGG
HaeIII 3 GGCC GG^CC
366 GGCC
MspA1I 110 CCGCTG C[AC]G^C[GT]G
155 CCGCTG
NlaIV 202 GGGTCC GG[ACGT]^[ACGT]CC
705 GGATCC
RcaI 399 TCATGA T^CATGA
543 TCATGA
ScrFI 206 CCGGG CC^[ACGT]GG
835 CCTGG
SfcI 482 CTACAG C^T[GA][CT]AG
957 CTATAG
Tsp45I 1021 GTCAC ^GT[GC]AC
1178 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BseRI BsrI
BstF5I BstYI DdeI DpnII EarI
Fnu4HI FokI HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
NdeII NlaIII PleI RsaI Sau3AI
SfaNI TfiI Tru9I TseI Tsp509I
XhoII