(Circular) (Six-base) MAPSORT of: pFN21AB3524.seq Check: 7723 from: 1 to: 6085 pFN21AB3524 6085 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2461 3895 278 Size: 53 83 186 936 925 1434 2468 Fragments arranged by size: 2468 1434 936 925 186 83 53 AccI GT'mk_AC Cuts at: 1937 2314 3428 1937 Size: 377 1114 4594 Fragments arranged by size: 4594 1114 377 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 2754 4212 4585 1563 Size: 1191 1458 373 3063 Fragments arranged by size: 3063 1458 1191 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2822 2948 3111 4148 5532 5800 1632 Size: 1190 126 163 1037 1384 268 1917 Fragments arranged by size: 1917 1384 1190 1037 268 163 126 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AlwNI CAG_nnn'CTG Cuts at: 2519 2840 3828 5400 2519 Size: 321 988 1572 3204 Fragments arranged by size: 3204 1572 988 321 ApaLI G'TGCA_C Cuts at: 4142 5298 4142 Size: 1156 4929 ApoI r'AATT_y Cuts at: 1493 2387 2399 2721 2867 3394 3620 3772 5881 Size: 894 12 322 146 527 226 152 2109 Cuts at: 5881 1493 Size: 1697 Fragments arranged by size: 2109 1697 894 527 322 226 152 146 12 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2084 3410 1870 Size: 73 141 1326 4545 Fragments arranged by size: 4545 1326 141 73 BamHI G'GATC_C Cuts at: 2710 3415 3782 2710 Size: 705 367 5013 Fragments arranged by size: 5013 705 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3406 4861 618 Size: 325 217 627 1619 1455 1842 Fragments arranged by size: 1842 1619 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2085 2850 3404 1391 Size: 694 765 554 4072 Fragments arranged by size: 4072 765 694 554 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3378 928 Size: 971 1479 3635 Fragments arranged by size: 3635 1479 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 1548 Size: 6085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 3502 3502 Size: 6085 Bme1580I G_kGCm'C Cuts at: 1790 4146 5302 1790 Size: 2356 1156 2573 Fragments arranged by size: 2573 2356 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3816 4774 442 Size: 416 53 83 399 24 2399 958 1753 Fragments arranged by size: 2399 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2942 1056 Size: 1886 4199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2706 4745 1239 Size: 878 589 2039 2579 Fragments arranged by size: 2579 2039 878 589 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3377 4210 5075 5373 3377 Size: 833 865 298 4089 Fragments arranged by size: 4089 865 833 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4748 882 Size: 617 3249 2219 Fragments arranged by size: 3249 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3194 3876 493 Size: 711 1990 682 2702 Fragments arranged by size: 2702 1990 711 682 BsaBI GATnn'nnATC Cuts at: 2955 6079 2955 Size: 3124 2961 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2458 3892 4274 4909 Size: 53 83 186 936 925 1434 382 635 Cuts at: 4909 275 Size: 1451 Fragments arranged by size: 1451 1434 936 925 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2752 2782 1178 Size: 30 165 30 1349 30 4481 Fragments arranged by size: 4481 1349 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2585 2660 2663 2666 2669 2672 2675 2678 Size: 1399 75 3 3 3 3 3 3 Cuts at: 2678 2720 3120 1186 Size: 42 400 4151 Fragments arranged by size: 4151 1399 400 75 42 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2120 2162 2204 5288 5784 2120 Size: 42 42 3084 496 2421 Fragments arranged by size: 3084 2421 496 42 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5512 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3535 4296 5324 1540 Size: 275 186 1534 761 1028 2301 Fragments arranged by size: 2301 1534 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4146 4231 5302 4146 Size: 85 1071 4929 Fragments arranged by size: 4929 1071 85 BsmI GAATG_Cn' Cuts at: 3596 3689 3596 Size: 93 5992 BsmBI CGTCTCn'nnnn_ Cuts at: 2124 2124 Size: 6085 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2850 3404 4146 4231 5302 1391 Size: 399 295 765 554 742 85 1071 2174 Fragments arranged by size: 2174 1071 765 742 554 399 295 85 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 2404 2548 3974 5704 2404 Size: 144 1426 1730 2785 Fragments arranged by size: 2785 1730 1426 144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3440 844 Size: 2596 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2369 3972 1394 Size: 429 546 1603 3507 Fragments arranged by size: 3507 1603 546 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4580 4754 65 Size: 1183 3332 174 1396 Fragments arranged by size: 3332 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4729 4916 1700 Size: 110 2919 187 2869 Fragments arranged by size: 2919 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 4145 5157 4145 Size: 1012 5073 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstYI r'GATC_y Cuts at: 1972 2710 2836 3063 3415 3782 4167 4184 5636 Size: 738 126 227 352 367 385 17 1452 Cuts at: 5636 5722 5734 6080 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 738 385 367 352 346 227 126 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2393 2715 2393 Size: 322 5763 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 3601 4395 4415 3601 Size: 794 20 5271 Fragments arranged by size: 5271 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 2748 3389 2748 Size: 641 5444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2370 3532 Size: 54 1425 50 275 18 177 363 1162 Cuts at: 3532 4421 6050 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1162 889 363 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 1537 Size: 275 1720 4090 Fragments arranged by size: 4090 1720 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 2487 2547 2637 2685 2697 4015 1353 Size: 693 441 60 90 48 12 1318 3423 Fragments arranged by size: 3423 1318 693 441 90 60 48 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4672 5046 5192 387 Size: 1064 138 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2706 2822 2948 3111 4148 4745 Size: 393 485 589 116 126 163 1037 597 Cuts at: 4745 5532 5800 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1037 787 597 589 485 393 268 163 126 116 EcoRI G'AATT_C Cuts at: 2721 3394 3772 2721 Size: 673 378 5034 Fragments arranged by size: 5034 673 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2846 2846 Size: 6085 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 5232 1678 Size: 3554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2448 2480 4726 Size: 32 135 32 662 32 182 32 2246 Cuts at: 4726 4758 4800 4832 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2246 662 182 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2910 3429 3675 669 Size: 988 281 972 519 246 3079 Fragments arranged by size: 3079 988 972 519 281 246 HindIII A'AGCT_T Cuts at: 748 2354 2828 3339 748 Size: 1606 474 511 3494 Fragments arranged by size: 3494 1606 511 474 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3324 3684 3324 Size: 360 5725 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5199 5383 1305 Size: 3894 184 2007 Fragments arranged by size: 3894 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4263 Fragments arranged by size: 4263 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2589 4043 4402 4561 518 Size: 648 1423 1454 359 159 2042 Fragments arranged by size: 2042 1454 1423 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2117 2162 3501 4178 5326 5571 1606 Size: 511 45 1339 677 1148 245 2120 Fragments arranged by size: 2120 1339 1148 677 511 245 45 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NheI G'CTAG_C Cuts at: 1052 2938 1052 Size: 1886 4199 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 3443 4988 1132 Size: 97 2214 1545 2229 Fragments arranged by size: 2229 2214 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PstI C_TGCA'G Cuts at: 830 3437 830 Size: 2607 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 2487 2962 3433 4568 5249 5440 826 Size: 219 1442 475 471 1135 681 191 1471 Fragments arranged by size: 1471 1442 1135 681 475 471 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3356 4189 5090 5352 820 Size: 197 926 1413 833 901 262 1553 Fragments arranged by size: 1553 1413 926 901 833 262 197 SnaBI TAC'GTA Cuts at: 493 3194 3876 493 Size: 2701 682 2702 Fragments arranged by size: 2702 2701 682 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 2891 4009 5 Size: 47 2839 1118 2081 Fragments arranged by size: 2839 2081 1118 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6085 StyI C'CwwG_G Cuts at: 513 1065 2004 3102 3524 513 Size: 552 939 1098 422 3074 Fragments arranged by size: 3074 1098 939 552 422 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4112 4129 4282 4467 4912 325 Size: 1255 2532 17 153 185 445 1498 Fragments arranged by size: 2532 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4331 6043 96 Size: 274 80 33 51 1443 2354 1712 138 Fragments arranged by size: 2354 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2131 2163 2215 2247 6045 6077 2131 Size: 32 52 32 3798 32 2139 Fragments arranged by size: 3798 2139 52 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6085 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 3096 4214 3096 Size: 1118 4967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2459 3893 276 Size: 53 83 186 936 925 1434 2468 Fragments arranged by size: 2468 1434 936 925 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI