Restriction map for
pFN21AB3524
(Linear) (Six-base) MAPSORT of: KIBB3524.seq Check: 9139 from: 1 to: 1380
KIBB3524.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 0 456 1380
Size: 456 924
AccI GT'mk_AC
Cuts at: 0 309 1380
Size: 309 1071
AclI AA'CG_TT
Cuts at: 0 749 1380
Size: 749 631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 943 1106 1380
Size: 817 126 163 274
Fragments arranged by size:
817 274 163 126
AlwNI CAG_nnn'CTG
Cuts at: 0 514 835 1380
Size: 514 321 545
Fragments arranged by size:
545 514 321
ApoI r'AATT_y
Cuts at: 0 382 394 716 862 1380
Size: 382 12 322 146 518
Fragments arranged by size:
518 382 322 146 12
AvaI C'yCGr_G
Cuts at: 0 79 1380
Size: 79 1301
BamHI G'GATC_C
Cuts at: 0 705 1380
Size: 705 675
BanII G_rGCy'C
Cuts at: 0 80 845 1380
Size: 80 765 535
Fragments arranged by size:
765 535 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 1373 1380
Size: 1373 7
BmtI G_CTAG'C
Cuts at: 0 937 1380
Size: 937 443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 701 1380
Size: 112 589 679
Fragments arranged by size:
679 589 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1380
Size: 1372 8
BsaAI yAC'GTr
Cuts at: 0 1189 1380
Size: 1189 191
BsaBI GATnn'nnATC
Cuts at: 0 950 1380
Size: 950 430
BsaHI Gr'CG_yC
Cuts at: 0 453 1380
Size: 453 927
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 747 777 1380
Size: 747 30 603
Fragments arranged by size:
747 603 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 655 658 661 664 667 670 673
Size: 580 75 3 3 3 3 3 3
Cuts at: 673 715 1115 1380
Size: 42 400 265
Fragments arranged by size:
580 400 265 75 42 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 115 157 199 1380
Size: 115 42 42 1181
Fragments arranged by size:
1181 115 42 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 119 1380
Size: 119 1261
Bsp1286I G_dGCh'C
Cuts at: 0 80 845 1380
Size: 80 765 535
Fragments arranged by size:
765 535 80
BspHI T'CATG_A
Cuts at: 0 399 543 1380
Size: 399 144 837
Fragments arranged by size:
837 399 144
BsrBI CCG'CTC
Cuts at: 0 364 1380
Size: 364 1016
BstYI r'GATC_y
Cuts at: 0 705 831 1058 1380
Size: 705 126 227 322
Fragments arranged by size:
705 322 227 126
Bsu36I CC'TnA_GG
Cuts at: 0 388 710 1380
Size: 388 322 670
Fragments arranged by size:
670 388 322
DraI TTT'AAA
Cuts at: 0 743 1380
Size: 743 637
EaeI y'GGCC_r
Cuts at: 0 2 365 1380
Size: 2 363 1015
Fragments arranged by size:
1015 363 2
EarI CTCTTCn'nnn_
Cuts at: 0 41 482 542 632 680 692 1380
Size: 41 441 60 90 48 12 688
Fragments arranged by size:
688 441 90 60 48 41 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 701 817 943 1106 1380
Size: 112 589 116 126 163 274
Fragments arranged by size:
589 274 163 126 116 112
EcoRI G'AATT_C
Cuts at: 0 716 1380
Size: 716 664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 841 1380
Size: 841 539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 443 475 1380
Size: 229 32 182 32 905
Fragments arranged by size:
905 229 182 32 32
HincII GTy'rAC
Cuts at: 0 905 1380
Size: 905 475
HindIII A'AGCT_T
Cuts at: 0 349 823 1334 1380
Size: 349 474 511 46
Fragments arranged by size:
511 474 349 46
MfeI C'AATT_G
Cuts at: 0 1319 1380
Size: 1319 61
MslI CAynn'nnrTG
Cuts at: 0 584 1380
Size: 584 796
MspA1I CmG'CkG
Cuts at: 0 112 157 1380
Size: 112 45 1223
Fragments arranged by size:
1223 112 45
NheI G'CTAG_C
Cuts at: 0 933 1380
Size: 933 447
SfcI C'TryA_G
Cuts at: 0 482 957 1380
Size: 482 475 423
Fragments arranged by size:
482 475 423
SmlI C'TyrA_G
Cuts at: 0 1351 1380
Size: 1351 29
SnaBI TAC'GTA
Cuts at: 0 1189 1380
Size: 1189 191
SspI AAT'ATT
Cuts at: 0 886 1380
Size: 886 494
StyI C'CwwG_G
Cuts at: 0 1097 1380
Size: 1097 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 210 242 1380
Size: 126 32 52 32 1138
Fragments arranged by size:
1138 126 52 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1091 1380
Size: 1091 289
ZraI GAC'GTC
Cuts at: 0 454 1380
Size: 454 926
Enzymes that do cut:
AatII AccI AclI AcuI AlwNI ApoI AvaI BamHI
BanII BbsI BmtI BpmI BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsmBI Bsp1286I BspHI BsrBI BstYI
Bsu36I DraI EaeI EarI Eco57MI EcoRI FalI Hin4I
HincII HindIII MfeI MslI MspA1I NheI SfcI SmlI
SnaBI SspI StyI TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BsaI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI StuI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI