(Circular) (Six-base) MAPSORT of: pFN21AE0970.seq Check: 2467 from: 1 to: 6274 pFN21AE0970 6274 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2856 4084 278 Size: 53 83 186 936 1320 1228 2468 Fragments arranged by size: 2468 1320 1228 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3595 3595 Size: 6274 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2369 3418 4337 5721 5989 1632 Size: 563 174 1049 919 1384 268 1917 Fragments arranged by size: 1917 1384 1049 919 563 268 174 AfeI AGC'GCT Cuts at: 3396 3396 Size: 6274 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 3372 5173 6150 1203 Size: 2169 1801 977 1327 Fragments arranged by size: 2169 1801 1327 977 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6274 AlwNI CAG_nnn'CTG Cuts at: 2302 3061 3106 3289 4017 5589 2302 Size: 759 45 183 728 1572 2987 Fragments arranged by size: 2987 1572 759 728 183 45 ApaLI G'TGCA_C Cuts at: 2225 2918 4331 5487 2225 Size: 693 1413 1156 3012 Fragments arranged by size: 3012 1413 1156 693 ApoI r'AATT_y Cuts at: 1493 3026 3583 3809 3961 6070 1493 Size: 1533 557 226 152 2109 1697 Fragments arranged by size: 2109 1697 1533 557 226 152 AseI AT'TA_AT Cuts at: 160 1032 4828 160 Size: 872 3796 1606 Fragments arranged by size: 3796 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 3599 1870 Size: 73 1656 4545 Fragments arranged by size: 4545 1656 73 BamHI G'GATC_C Cuts at: 3604 3971 3604 Size: 367 5907 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 3341 3595 5050 618 Size: 325 217 627 489 1065 254 1455 1842 Fragments arranged by size: 1842 1455 1065 627 489 325 254 217 BanII G_rGCy'C Cuts at: 1391 2259 2311 2557 2564 3498 3593 1391 Size: 868 52 246 7 934 95 4072 Fragments arranged by size: 4072 934 868 246 95 52 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3308 928 Size: 971 1409 3894 Fragments arranged by size: 3894 1409 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4465 4499 1903 Size: 34 2528 34 3678 Fragments arranged by size: 3678 2528 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 1548 Size: 6274 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2480 2877 Size: 107 122 71 631 605 343 465 397 Cuts at: 2877 4885 136 Size: 2008 1525 Fragments arranged by size: 2008 1525 631 605 465 397 343 122 107 71 BglII A'GATC_T Cuts at: 2216 2413 2862 3447 6269 2216 Size: 197 449 585 2822 2221 Fragments arranged by size: 2822 2221 585 449 197 BlpI GC'TnA_GC Cuts at: 3402 3691 3402 Size: 289 5985 Bme1580I G_kGCm'C Cuts at: 1790 2229 2279 2922 3344 4335 5491 1790 Size: 439 50 643 422 991 1156 2573 Fragments arranged by size: 2573 1156 991 643 439 422 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2770 3116 4005 Size: 416 53 83 399 24 1353 346 889 Cuts at: 4005 4963 442 Size: 958 1753 Fragments arranged by size: 1753 1353 958 889 416 399 346 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3401 1056 Size: 2345 3929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4934 1239 Size: 3695 2579 Bpu10I CC'TnA_GC Cuts at: 2723 2723 Size: 6274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2006 4399 5264 5562 2006 Size: 2393 865 298 2718 Fragments arranged by size: 2718 2393 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4937 882 Size: 617 3438 2219 Fragments arranged by size: 3438 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3274 4065 493 Size: 711 2070 791 2702 Fragments arranged by size: 2702 2070 791 711 BsaBI GATnn'nnATC Cuts at: 6268 6268 Size: 6274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2245 2853 4081 4463 Size: 53 83 186 936 712 608 1228 382 Cuts at: 4463 5098 275 Size: 635 1451 Fragments arranged by size: 1451 1228 936 712 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6049 Fragments arranged by size: 6049 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2345 3254 1186 Size: 1027 132 909 4206 Fragments arranged by size: 4206 1027 909 132 BseYI C'CCAG_C Cuts at: 5477 5973 5477 Size: 496 5778 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 1163 Size: 573 606 5095 Fragments arranged by size: 5095 606 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3158 3724 4485 5513 1540 Size: 275 186 1157 566 761 1028 2301 Fragments arranged by size: 2301 1157 1028 761 566 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2259 2564 2592 2922 3408 3498 4335 4420 Size: 30 305 28 330 486 90 837 85 Cuts at: 4420 5491 2229 Size: 1071 3012 Fragments arranged by size: 3012 1071 837 486 330 305 90 85 30 28 BsmI GAATG_Cn' Cuts at: 2704 3785 3878 2704 Size: 1081 93 5100 Fragments arranged by size: 5100 1081 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2252 2252 Size: 6274 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2259 2279 2311 2557 2564 2592 Size: 399 439 30 20 32 246 7 28 Cuts at: 2592 2922 3344 3408 3498 3593 4335 4420 5491 Size: 330 422 64 90 95 742 85 1071 Cuts at: 5491 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 439 422 399 330 246 95 90 85 64 32 30 28 20 7 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 4163 5893 4163 Size: 1730 4544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2670 2803 3321 3629 844 Size: 1826 133 518 308 3489 Fragments arranged by size: 3489 1826 518 308 133 BsrBI CCG'CTC Cuts at: 1394 1823 2779 4161 1394 Size: 429 956 1382 3507 Fragments arranged by size: 3507 1382 956 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2947 4769 4943 65 Size: 1183 1699 1822 174 1396 Fragments arranged by size: 1822 1699 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4918 5105 1700 Size: 110 3108 187 2869 Fragments arranged by size: 3108 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6274 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6274 BstBI TT'CG_AA Cuts at: 5078 5078 Size: 6274 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstYI r'GATC_y Cuts at: 1972 2216 2391 2413 2427 2862 3118 3447 3604 Size: 244 175 22 14 435 256 329 157 Cuts at: 3604 3971 4356 4373 5825 5911 5923 6269 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 435 385 367 346 329 256 244 175 157 86 22 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2772 2998 513 Size: 552 939 768 226 3789 Fragments arranged by size: 3789 939 768 552 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5535 Fragments arranged by size: 5535 683 56 BtsI GCAGTG_nn' Cuts at: 2505 2920 3198 3790 4584 4604 2505 Size: 415 278 592 794 20 4175 Fragments arranged by size: 4175 794 592 415 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 3578 3578 Size: 6274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2523 3234 Size: 54 1425 50 275 18 177 516 711 Cuts at: 3234 3721 4610 6239 8 Size: 487 889 1629 43 Fragments arranged by size: 1629 1425 889 711 516 487 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3074 4204 1353 Size: 818 903 1130 3423 Fragments arranged by size: 3423 1130 903 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2384 2530 2993 3020 4861 5235 Size: 1064 138 795 146 463 27 1841 374 Cuts at: 5235 5381 387 Size: 146 1280 Fragments arranged by size: 1841 1280 1064 795 463 374 146 146 138 27 EcoICRI GAG'CTC Cuts at: 2257 2562 3496 2257 Size: 305 934 5035 Fragments arranged by size: 5035 934 305 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2369 3418 4337 4934 5721 5989 Size: 393 563 174 1049 919 597 787 268 Cuts at: 5989 1239 Size: 1524 Fragments arranged by size: 1524 1049 919 787 597 563 393 268 174 EcoNI CCTnn'n_nnAGG Cuts at: 2483 2582 2483 Size: 99 6175 EcoO109I rG'GnC_Cy Cuts at: 2746 2764 2782 3441 2746 Size: 18 18 659 5579 Fragments arranged by size: 5579 659 18 18 EcoRI G'AATT_C Cuts at: 3026 3583 3961 3026 Size: 557 378 5339 Fragments arranged by size: 5339 557 378 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 2112 3357 3398 5421 1678 Size: 434 1245 41 2023 2531 Fragments arranged by size: 2531 2023 1245 434 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2696 2728 3244 3276 4915 Size: 32 135 32 1124 32 516 32 1639 Cuts at: 4915 4947 4989 5021 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1639 1124 516 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3172 3618 3864 669 Size: 988 281 1234 446 246 3079 Fragments arranged by size: 3079 1234 988 446 281 246 HindIII A'AGCT_T Cuts at: 748 2024 748 Size: 1276 4998 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3599 3599 Size: 6274 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2973 5388 5572 1305 Size: 1668 2415 184 2007 Fragments arranged by size: 2415 2007 1668 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4275 Fragments arranged by size: 4275 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 4232 4591 4750 518 Size: 648 1122 1944 359 159 2042 Fragments arranged by size: 2042 1944 1122 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2611 3690 4367 5515 5760 1606 Size: 606 399 1079 677 1148 245 2120 Fragments arranged by size: 2120 1148 1079 677 606 399 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2998 513 Size: 552 939 994 3789 Fragments arranged by size: 3789 994 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6274 NheI G'CTAG_C Cuts at: 1052 3397 1052 Size: 2345 3929 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2386 3145 3376 3632 5177 1132 Size: 97 1157 759 231 256 1545 2229 Fragments arranged by size: 2229 1545 1157 759 256 231 97 PasI CC'CwG_GG Cuts at: 1348 3337 1348 Size: 1989 4285 PciI A'CATG_T Cuts at: 3372 5173 3372 Size: 1801 4473 PflMI CCAn_nnn'nTGG Cuts at: 1108 2302 3106 1108 Size: 1194 804 4276 Fragments arranged by size: 4276 1194 804 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PmlI CAC'GTG Cuts at: 3274 3274 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2746 2764 2782 3441 2746 Size: 18 18 659 5579 Fragments arranged by size: 5579 659 18 18 PshAI GACnn'nnGTC Cuts at: 1613 2144 1613 Size: 531 5743 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PstI C_TGCA'G Cuts at: 830 3626 830 Size: 2796 3478 PvuI CG_AT'CG Cuts at: 2001 3158 2001 Size: 1157 5117 PvuII CAG'CTG Cuts at: 2212 2611 2212 Size: 399 5875 SacI G_AGCT'C Cuts at: 2259 2564 3498 2259 Size: 305 934 5035 Fragments arranged by size: 5035 934 305 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 2087 2204 4522 6234 2087 Size: 117 2318 1712 2127 Fragments arranged by size: 2318 2127 1712 117 SfcI C'TryA_G Cuts at: 826 1045 2074 3622 4757 5438 5629 826 Size: 219 1029 1548 1135 681 191 1471 Fragments arranged by size: 1548 1471 1135 1029 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2480 2480 Size: 6274 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 3601 1872 Size: 1729 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 4378 5279 5541 820 Size: 197 926 78 2357 901 262 1553 Fragments arranged by size: 2357 1553 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 4065 493 Size: 3572 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 2386 3145 3632 1132 Size: 1254 759 487 3774 Fragments arranged by size: 3774 1254 759 487 SspI AAT'ATT Cuts at: 5 52 4198 5 Size: 47 4146 2081 Fragments arranged by size: 4146 2081 47 StuI AGG'CCT Cuts at: 1517 3464 1517 Size: 1947 4327 StyI C'CwwG_G Cuts at: 513 1065 2004 2998 3713 513 Size: 552 939 994 715 3074 Fragments arranged by size: 3074 994 939 715 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2556 3157 4301 4318 4471 4656 5101 Size: 1255 976 601 1144 17 153 185 445 Cuts at: 5101 325 Size: 1498 Fragments arranged by size: 1498 1255 1144 976 601 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2085 2202 2334 Size: 274 80 33 51 1443 108 117 132 Cuts at: 2334 2944 3093 4520 6232 96 Size: 610 149 1427 1712 138 Fragments arranged by size: 1712 1443 1427 610 274 149 138 132 117 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6234 6266 6234 Size: 32 6242 Tth111I GACn'n_nGTC Cuts at: 1316 3167 3533 1316 Size: 1851 366 4057 Fragments arranged by size: 4057 1851 366 XbaI T'CTAG_A Cuts at: 3610 3610 Size: 6274 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6274 XmaI C'CCGG_G Cuts at: 1870 3599 1870 Size: 1729 4545 XmnI GAAnn'nnTTC Cuts at: 4403 4403 Size: 6274 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2854 4082 276 Size: 53 83 186 936 1320 1228 2468 Fragments arranged by size: 2468 1320 1228 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI