Restriction map for
pFN21AE0934
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NgoMIV NotI NruI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 601 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 601 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1141 CTCGAG C^[CT]CG[GA]G
AvaII 340 GGTCC G^G[AT]CC
BanII 921 GGGCTC G[GA]GC[CT]^C
BbvI 439 GCTGC GCAGC(8/12)
BcnI 1177 CCCGG CC^[GC]GG
BglII 1032 AGATCT A^GATCT
BmyI 921 GGGCTC G[AGT]GC[ACT]^C
BsaBI 661 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 984 CGTACG C^GTACG
BsiYI 6 CCACTCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 6 CCACTCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1175 GTCCC GGGAC(10/14)
BsmI 405 GCATTC GAATGC(1/-1)
BsoBI 1141 CTCGAG C^[CT]CG[GA]G
Bsp1286I 921 GGGCTC G[AGT]GC[ACT]^C
BspMI 1074 ACCTGC ACCTGC(4/8)
BsrFI 12 GCCGGT [GA]^CCGG[CT]
BsrI 321 ACTGG ACTGG(1/-1)
BstXI 24 CCAAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 84 GCGC GCG^C
Cfr10I 12 GCCGGT [GA]^CCGG[CT]
Eam1105I 601 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 638 CCAAGG C^C[AT][AT]GG
EcoT22I 664 ATGCAT ATGCA^T
HaeIII 3 GGCC GG^CC
HhaI 84 GCGC GCG^C
HinP1I 84 GCGC G^CGC
MamI 661 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 895 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 502 CAGCTG C[AC]G^C[GT]G
NciI 1177 CCCGG CC^[GC]GG
NdeI 239 CATATG CA^TATG
NheI 1114 GCTAGC G^CTAGC
NlaIII 369 CATG CATG^
NlaIV 558 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 664 ATGCAT ATGCA^T
PaeR7I 1141 CTCGAG C^TCGAG
PvuII 502 CAGCTG CAG^CTG
SfcI 788 CTACAG C^T[GA][CT]AG
SgrAI 11 CGCCGGTG C[GA]^CCGG[CT]G
StyI 638 CCAAGG C^C[AT][AT]GG
TseI 439 GCTGC G^C[AT]GC
Tsp45I 230 GTGAC ^GT[GC]AC
XhoI 1141 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 1169 CCGC CCGC(-3/-1)
308 GCGG
BbsI 193 GAAGAC GAAGAC(2/6)
837 GAAGAC
BfaI 634 CTAG C^TAG
1115 CTAG
BpuAI 193 GAAGAC GAAGAC(2/6)
837 GAAGAC
BsmAI 428 GAGAC GTCTC(1/5)
722 GAGAC
BstNI 31 CCTGG CC^[AT]GG
303 CCTGG
Cac8I 757 GCTTGC GC[ACGT]^[ACGT]GC
1114 GCTAGC
Cfr13I 3 GGCCC G^G[ACGT]CC
340 GGTCC
DraI 267 TTTAAA TTT^AAA
384 TTTAAA
EcoRII 31 CCTGG ^CC[AT]GG
303 CCTGG
Fnu4HI 439 GCTGC GC^[ACGT]GC
1168 GCCGC
HapII 13 CCGG C^CGG
1178 CCGG
HpaII 13 CCGG C^CGG
1178 CCGG
HphI 1197 GGTGA GGTGA(8/7)
423 TCACC
ItaI 439 GCTGC GC^[ACGT]GC
1168 GCCGC
MaeI 634 CTAG C^TAG
1115 CTAG
MaeIII 230 GTGAC ^GT[ACGT]AC
244 GTAAC
MspI 13 CCGG C^CGG
1178 CCGG
MvaI 31 CCTGG CC^[AT]GG
303 CCTGG
MwoI 30 GCCTGGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1162 GCTGAAGCCGC
PleI 589 GACTC GAGTC(4/5)
222 GAGTC
Sau96I 3 GGCCC G^G[ACGT]CC
340 GGTCC
TaqI 333 TCGA T^CGA
1142 TCGA
TspRI 320 CACTG CA[GC]TG[ACGT][ACGT]^
897 CAGTG
TthHB8I 333 TCGA T^CGA
1142 TCGA
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
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AcsI AfaI AluI AlwI ApoI
Asp700I BsaJI BssKI BstF5I BstYI
DdeI DpnII FokI HinfI MboI
MboII MflI MnlI MseI NdeII
RsaI Sau3AI ScrFI SfaNI SmlI
TfiI Tru9I Tsp509I XhoII XmnI