Restriction map for
pFN21AE0934
(Linear) (Six-base) MAPSORT of: KIEE0934.seq Check: 7610 from: 1 to: 1248
KIEE0934.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1083 1248
Size: 1083 165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1184 1248
Size: 584 600 64
Fragments arranged by size:
600 584 64
AhdI GACnn_n'nnGTC
Cuts at: 0 606 1248
Size: 606 642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 164 1248
Size: 164 1084
ApoI r'AATT_y
Cuts at: 0 380 419 814 828 1216 1248
Size: 380 39 395 14 388 32
Fragments arranged by size:
395 388 380 39 32 14
AvaI C'yCGr_G
Cuts at: 0 1141 1248
Size: 1141 107
BanII G_rGCy'C
Cuts at: 0 925 1248
Size: 925 323
BbsI GAAGACnn'nnnn_
Cuts at: 0 200 844 1248
Size: 200 644 404
Fragments arranged by size:
644 404 200
BfrBI ATG'CAT
Cuts at: 0 666 1248
Size: 666 582
BglII A'GATC_T
Cuts at: 0 1032 1248
Size: 1032 216
BmtI G_CTAG'C
Cuts at: 0 1118 1248
Size: 1118 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 240 1248
Size: 190 50 1008
Fragments arranged by size:
1008 190 50
BsaBI GATnn'nnATC
Cuts at: 0 665 1248
Size: 665 583
BseYI C'CCAG_C
Cuts at: 0 137 1248
Size: 137 1111
BsiWI C'GTAC_G
Cuts at: 0 984 1248
Size: 984 264
BsmI GAATG_Cn'
Cuts at: 0 405 1248
Size: 405 843
Bsp1286I G_dGCh'C
Cuts at: 0 925 1248
Size: 925 323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1083 1248
Size: 1083 165
BsrFI r'CCGG_y
Cuts at: 0 12 1248
Size: 12 1236
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 1248
Size: 31 1217
BstYI r'GATC_y
Cuts at: 0 324 771 1032 1248
Size: 324 447 261 216
Fragments arranged by size:
447 324 261 216
DraI TTT'AAA
Cuts at: 0 269 386 1248
Size: 269 117 862
Fragments arranged by size:
862 269 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1184 1248
Size: 584 600 64
Fragments arranged by size:
600 584 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 831 863 892 919 924 951
Size: 592 32 207 32 29 27 5 27
Cuts at: 951 1248
Size: 297
Fragments arranged by size:
592 297 207 32 32 29 27 27
5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 733 1248
Size: 699 34 515
Fragments arranged by size:
699 515 34
MslI CAynn'nnrTG
Cuts at: 0 899 1248
Size: 899 349
MspA1I CmG'CkG
Cuts at: 0 504 1248
Size: 504 744
NdeI CA'TA_TG
Cuts at: 0 240 1248
Size: 240 1008
NheI G'CTAG_C
Cuts at: 0 1114 1248
Size: 1114 134
NsiI A_TGCA'T
Cuts at: 0 668 1248
Size: 668 580
PvuII CAG'CTG
Cuts at: 0 504 1248
Size: 504 744
SfcI C'TryA_G
Cuts at: 0 788 1248
Size: 788 460
SgrAI Cr'CCGG_yG
Cuts at: 0 12 1248
Size: 12 1236
SmlI C'TyrA_G
Cuts at: 0 169 219 1141 1248
Size: 169 50 922 107
Fragments arranged by size:
922 169 107 50
StyI C'CwwG_G
Cuts at: 0 638 1248
Size: 638 610
TatI w'GTAC_w
Cuts at: 0 1011 1248
Size: 1011 237
XhoI C'TCGA_G
Cuts at: 0 1141 1248
Size: 1141 107
XmnI GAAnn'nnTTC
Cuts at: 0 197 569 1217 1248
Size: 197 372 648 31
Fragments arranged by size:
648 372 197 31
Enzymes that do cut:
AarI AcuI AhdI AlfI ApoI AvaI BanII BbsI
BfrBI BglII BmtI BpuEI BsaBI BseYI BsiWI BsmI
Bsp1286I BspMI BsrFI BstXI BstYI DraI Eco57MI Hin4I
MmeI MslI MspA1I NdeI NheI NsiI PvuII SfcI
SgrAI SmlI StyI TatI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NotI NruI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XmaI ZraI