Restriction map for
pFN21AE0824
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HindIII KpnI KspI MaeI MaeII
MaeIII MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp45I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AciI 570 CCGC CCGC(-3/-1)
AcsI 410 AAATTC [GA]^AATT[CT]
AcyI 766 GGCGCC G[GA]^CG[CT]C
AflIII 80 ACATGT A^C[GA][CT]GT
Alw44I 291 GTGCAC G^TGCAC
ApaI 379 GGGCCC GGGCC^C
ApaLI 291 GTGCAC G^TGCAC
ApoI 410 AAATTC [GA]^AATT[CT]
AspI 792 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 383 CCCGGG C^[CT]CG[GA]G
BanI 766 GGCGCC G^G[CT][GA]CC
BanII 379 GGGCCC G[GA]GC[CT]^C
BbeI 766 GGCGCC GGCGC^C
BbvI 193 GCTGC GCAGC(8/12)
BsaHI 766 GGCGCC G[GA]^CG[CT]C
BsmAI 517 GTCTC GTCTC(1/5)
BsmI 750 GAATGC GAATGC(1/-1)
BsoBI 383 CCCGGG C^[CT]CG[GA]G
BspHI 275 TCATGA T^CATGA
BspLU11I 80 ACATGT A^CATGT
BspMI 589 ACCTGC ACCTGC(4/8)
BsrI 261 CCAGT ACTGG(1/-1)
BstXI 107 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 551 AGATCC [GA]^GATC[CT]
Cac8I 429 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 767 GCGC GCG^C
DraII 379 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 11 GACTCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 635 CCATGG C^C[GA][CT]GG
EcoNI 227 CCTATGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 379 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 635 CCATGG C^C[AT][AT]GG
EcoT22I 159 ATGCAT ATGCA^T
HaeII 766 GGCGCC [GA]GCGC^[CT]
HapII 384 CCGG C^CGG
HgaI 792 GACGC GACGC(5/10)
HhaI 767 GCGC GCG^C
Hin1I 766 GGCGCC G[GA]^CG[CT]C
HinP1I 767 GCGC G^CGC
HincII 451 GTTAAC GT[CT]^[GA]AC
HindII 451 GTTAAC GT[CT]^[GA]AC
HpaI 451 GTTAAC GTT^AAC
HpaII 384 CCGG C^CGG
KasI 766 GGCGCC G^GCGCC
MflI 551 AGATCC [GA]^GATC[CT]
MseI 452 TTAA T^TAA
MspI 384 CCGG C^CGG
MwoI 786 GCAGAGGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 766 GGCGCC GG^CGCC
NcoI 635 CCATGG C^CATGG
NsiI 159 ATGCAT ATGCA^T
PflFI 792 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 784 CTGCAG CTGCA^G
RcaI 275 TCATGA T^CATGA
SfaNI 510 GATGC GCATC(5/9)
SfoI 766 GGCGCC GGC^GCC
SmaI 383 CCCGGG CCC^GGG
SmlI 424 CTCAAG C^T[CT][GA]AG
StuI 578 AGGCCT AGG^CCT
StyI 635 CCATGG C^C[AT][AT]GG
TaqI 586 TCGA T^CGA
Tru9I 452 TTAA T^TAA
TseI 193 GCTGC G^C[AT]GC
Tth111I 792 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 586 TCGA T^CGA
XhoII 551 AGATCC [GA]^GATC[CT]
XmaCI 383 CCCGGG C^CCGGG
XmaI 383 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 280 AGCT AG^CT
428 AGCT
AlwI 297 GATCC GGATC(4/5)
552 GATCC
AspHI 291 GTGCAC G[AT]GC[AT]^C
779 GAGCAC
AvaII 24 GGACC G^G[AT]CC
496 GGACC
BalI 68 TGGCCA TGG^CCA
242 TGGCCA
BcnI 383 CCCGG CC^[GC]GG
384 CCGGG
BpmI 259 CTCCAG CTGGAG(16/14)
282 CTGGAG
BseRI 594 CTCCTC GAGGAG(10/8)
673 GAGGAG
BsiHKAI 291 GTGCAC G[AT]GC[AT]^C
779 GAGCAC
EaeI 68 TGGCCA [CT]^GGCC[GA]
242 TGGCCA
EarI 400 CTCTTC CTCTTC(1/4)
619 CTCTTC
Fnu4HI 193 GCTGC GC^[ACGT]GC
569 GCCGC
HphI 219 GGTGA GGTGA(8/7)
759 GGTGA
ItaI 193 GCTGC GC^[ACGT]GC
569 GCCGC
Ksp632I 400 CTCTTC CTCTTC(1/4)
619 CTCTTC
MluNI 68 TGGCCA TGG^CCA
242 TGGCCA
MscI 68 TGGCCA TGG^CCA
242 TGGCCA
NciI 383 CCCGG CC^[GC]GG
384 CCGGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII FokI
HaeIII HinfI MboI MboII MnlI
MslI MvaI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfcI Tsp509I TspRI