Restriction map for
pFN21AE0815
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# NO CUT ENZYMES ( 198 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MseI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TaqI Tru9I TseI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AciI 198 CCGC CCGC(-3/-1)
AcsI 18 AAATTT [GA]^AATT[CT]
AfaI 3 GTAC GT^AC
AlwNI 509 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 18 AAATTT [GA]^AATT[CT]
BalI 65 TGGCCA TGG^CCA
BanI 445 GGCACC G^G[CT][GA]CC
BsmFI 361 GTCCC GGGAC(10/14)
Bsp1407I 2 TGTACA T^GTACA
BsrGI 2 TGTACA T^GTACA
BstF5I 500 GGATG GGATG(2/0)
Cfr13I 325 GGCCC G^G[ACGT]CC
EaeI 65 TGGCCA [CT]^GGCC[GA]
EcoNI 173 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 216 ATGCAT ATGCA^T
Fnu4HI 197 GCCGC GC^[ACGT]GC
FokI 500 GGATG GGATG(9/13)
HindIII 527 AAGCTT A^AGCTT
ItaI 197 GCCGC GC^[ACGT]GC
MaeII 207 ACGT A^CGT
MaeIII 562 GTCAC ^GT[ACGT]AC
MluNI 65 TGGCCA TGG^CCA
MscI 65 TGGCCA TGG^CCA
NlaIV 445 GGCACC GG[ACGT]^[ACGT]CC
NsiI 216 ATGCAT ATGCA^T
RsaI 3 GTAC GT^AC
Sau96I 325 GGCCC G^G[ACGT]CC
SspBI 2 TGTACA T^GTACA
SspI 331 AATATT AAT^ATT
Tsp45I 562 GTCAC ^GT[GC]AC
TspRI 240 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 355 GATCC GGATC(4/5)
607 GGATC
BbsI 388 GAAGAC GAAGAC(2/6)
433 GTCTTC
BpuAI 388 GAAGAC GAAGAC(2/6)
433 GTCTTC
BsiYI 173 CCTTTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
285 CCTAACTCTGG
BslI 173 CCTTTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
285 CCTAACTCTGG
BspHI 101 TCATGA T^CATGA
221 TCATGA
BsrI 241 ACTGG ACTGG(1/-1)
619 CCAGT
BssKI 30 CCTGG ^CC[ACGT]GG
604 CCTGG
BstNI 30 CCTGG CC^[AT]GG
604 CCTGG
Cac8I 63 GCTGGC GC[ACGT]^[ACGT]GC
514 GCTGGC
DpnII 355 GATC ^GATC
608 GATC
EcoRII 30 CCTGG ^CC[AT]GG
604 CCTGG
HinfI 297 GATTC G^A[ACGT]TC
483 GATTC
MboI 355 GATC ^GATC
608 GATC
MslI 260 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
585 CATGGTGATG
MvaI 30 CCTGG CC^[AT]GG
604 CCTGG
NdeII 355 GATC ^GATC
608 GATC
RcaI 101 TCATGA T^CATGA
221 TCATGA
Sau3AI 355 GATC ^GATC
608 GATC
ScrFI 30 CCTGG CC^[ACGT]GG
604 CCTGG
TfiI 297 GATTC G^A[AT]TC
483 GATTC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AluI HaeIII HphI MboII MnlI
NlaIII SfaNI Tsp509I