Restriction map for
pFN21AE0505
(Circular) (Six-base) MAPSORT of: pFN21AE0505.seq Check: 5382 from: 1 to: 5458
pFN21AE0505 5458 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2413 2413
Size: 5458
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 2340 2433 3521 4905 5173 1632
Size: 611 97 93 1088 1384 268 1917
Fragments arranged by size:
1917 1384 1088 611 268 97 93
AfeI AGC'GCT
Cuts at: 2258 2258
Size: 5458
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 2604 4187 2604
Size: 1583 3875
AleI CACnn'nnGTG
Cuts at: 1166 2346 2439 1166
Size: 1180 93 4185
Fragments arranged by size:
4185 1180 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2352 2445 1184
Size: 1168 93 4197
Fragments arranged by size:
4197 1168 93
AlwNI CAG_nnn'CTG
Cuts at: 3201 4773 3201
Size: 1572 3886
ApaI G_GGCC'C
Cuts at: 2036 2036
Size: 5458
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2767 2993 3145 5254 1493
Size: 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2783 1870
Size: 73 840 4545
Fragments arranged by size:
4545 840 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2779 4234 618
Size: 325 217 627 992 1455 1842
Fragments arranged by size:
1842 1455 992 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2777 1391
Size: 645 741 4072
Fragments arranged by size:
4072 741 645
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2213 2547 2595 2643 928
Size: 971 314 334 48 48 3743
Fragments arranged by size:
3743 971 334 314 48 48
BbvCI CC'TCA_GC
Cuts at: 2195 2195
Size: 5458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2509 5453 2509
Size: 2944 2514
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 2036 3519 4675 1790
Size: 246 1483 1156 2573
Fragments arranged by size:
2573 1483 1156 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4118 1239
Size: 2879 2579
Bpu10I CC'TnA_GC
Cuts at: 2195 2195
Size: 5458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2612 2712 4121 882
Size: 617 1113 100 1409 2219
Fragments arranged by size:
2219 1409 1113 617 100
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2244 2333 3265
Size: 53 83 186 936 677 34 89 932
Cuts at: 3265 3647 4282 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 932 677 635 382 186 89
83 53 34
BsaWI w'CCGG_w
Cuts at: 2650 3891 4289 4563 4710 5121 2650
Size: 1241 398 274 147 411 2987
Fragments arranged by size:
2987 1241 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 1178
Size: 30 165 30 831 30 4372
Fragments arranged by size:
4372 831 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2462 1186
Size: 1183 93 4182
Fragments arranged by size:
4182 1183 93
BseYI C'CCAG_C
Cuts at: 2036 2688 4661 5157 2036
Size: 652 1973 496 2337
Fragments arranged by size:
2337 1973 652 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2322 2348 2415 2441 1163
Size: 573 586 26 67 26 4180
Fragments arranged by size:
4180 586 573 67 26 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3519 3604 4675 3519
Size: 85 1071 4302
Fragments arranged by size:
4302 1071 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
BsmBI CGTCTCn'nnnn_
Cuts at: 2272 2272
Size: 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2777 3519 3604 4675 1391
Size: 399 246 741 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 399 246 85
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 2547 2595 2643 3347 5077 2547
Size: 48 48 704 1730 2928
Fragments arranged by size:
2928 1730 704 48 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2413 2813 844
Size: 1569 400 3489
Fragments arranged by size:
3489 1569 400
BsrBI CCG'CTC
Cuts at: 1394 1823 2250 3345 1394
Size: 429 427 1095 3507
Fragments arranged by size:
3507 1095 429 427
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4102 4289 1700
Size: 110 2292 187 2869
Fragments arranged by size:
2869 2292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2147 794
Size: 1353 4105
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2239
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2509 2557 2788 3155 3540 3557 5009 5095
Size: 537 48 231 367 385 17 1452 86
Cuts at: 5095 5107 5453 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 537 385 367 346 231 86
48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2385 2385
Size: 5458
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3967
Fragments arranged by size:
3967 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2136 2974 3768 3788 2136
Size: 838 794 20 3806
Fragments arranged by size:
3806 838 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2905 3794 5423
Size: 54 1425 50 275 18 1075 889 1629
Cuts at: 5423 8
Size: 43
Fragments arranged by size:
1629 1425 1075 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3388 1353
Size: 2035 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4045 4419 4565 387
Size: 1064 138 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2340 2433 3521 4118 4905 5173
Size: 393 611 97 93 1088 597 787 268
Cuts at: 5173 1239
Size: 1524
Fragments arranged by size:
1524 1088 787 611 597 393 268 97
93
EcoO109I rG'GnC_Cy
Cuts at: 2033 2044 2078 2312 2033
Size: 11 34 234 5179
Fragments arranged by size:
5179 234 34 11
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 2260 4605 1678
Size: 582 2345 2531
Fragments arranged by size:
2531 2345 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2281 2313 2542
Size: 32 135 32 662 32 15 32 229
Cuts at: 2542 2574 2590 2622 4099 4131 4173 4205 1373
Size: 32 16 32 1477 32 42 32 2626
Fragments arranged by size:
2626 1477 662 229 135 42 32 32
32 32 32 32 32 32 16 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4572 4756 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2091 2346 2439 3416 3775 3934 518
Size: 648 925 255 93 977 359 159 2042
Fragments arranged by size:
2042 977 925 648 359 255 159 93
MspA1I CmG'CkG
Cuts at: 1606 2040 2874 3551 4699 4944 1606
Size: 434 834 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 434 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3967
Fragments arranged by size:
3967 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2259 2500 1108
Size: 1151 241 4066
Fragments arranged by size:
4066 1151 241
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2078 2312 2078
Size: 234 5224
PshAI GACnn'nnGTC
Cuts at: 1613 2403 1613
Size: 790 4668
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PspOMI G'GGCC_C
Cuts at: 2032 2032
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2066 2810 830
Size: 1236 744 3478
Fragments arranged by size:
3478 1236 744
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SanDI GG'GwC_CC
Cuts at: 2078 2078
Size: 5458
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2062 2806 3941 4622 4813 826
Size: 219 1017 744 1135 681 191 1471
Fragments arranged by size:
1471 1135 1017 744 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3562 4463 4725 820
Size: 197 926 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2004 2897 513
Size: 552 939 893 3074
Fragments arranged by size:
3074 939 893 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 5416 96
Size: 274 80 33 51 1443 1727 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5418 5450 5418
Size: 32 5426
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2299 2794 2299
Size: 495 4963
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI