(Circular) (Six-base) MAPSORT of: pFN21AB9908.seq Check: 7329 from: 1 to: 6472 pFN21AB9908 6472 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3643 3643 Size: 6472 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4282 278 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2545 2960 3655 4535 5919 6187 1632 Size: 913 415 695 880 1384 268 1917 Fragments arranged by size: 1917 1384 913 880 695 415 268 AfeI AGC'GCT Cuts at: 3236 3236 Size: 6472 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 3090 5371 6348 1203 Size: 1887 2281 977 1327 Fragments arranged by size: 2281 1887 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6472 AlwNI CAG_nnn'CTG Cuts at: 2401 2716 3574 4215 5787 2401 Size: 315 858 641 1572 3086 Fragments arranged by size: 3086 1572 858 641 315 ApaLI G'TGCA_C Cuts at: 4529 5685 4529 Size: 1156 5316 ApoI r'AATT_y Cuts at: 1493 2326 3781 4007 4159 6268 1493 Size: 833 1455 226 152 2109 1697 Fragments arranged by size: 2109 1697 1455 833 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2049 2150 2945 3014 3218 3746 Size: 73 69 37 101 795 69 204 528 Cuts at: 3746 3797 1870 Size: 51 4545 Fragments arranged by size: 4545 795 528 204 101 73 69 69 51 37 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2193 3044 3793 5248 618 Size: 325 217 627 406 851 749 1455 1842 Fragments arranged by size: 1842 1455 851 749 627 406 325 217 BanII G_rGCy'C Cuts at: 1391 2013 3722 3791 1391 Size: 622 1709 69 4072 Fragments arranged by size: 4072 1709 622 69 BbeI G_GCGC'C Cuts at: 2197 2197 Size: 6472 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2863 928 Size: 971 964 4537 Fragments arranged by size: 4537 971 964 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2017 2051 3132 3166 4663 4697 1903 Size: 34 80 34 1081 34 1497 34 3678 Fragments arranged by size: 3678 1497 1081 80 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 1548 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2191 2847 5083 Size: 107 122 71 631 605 519 656 2236 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 2236 1525 656 631 605 519 122 107 71 BglII A'GATC_T Cuts at: 2886 6467 2886 Size: 3581 2891 BlpI GC'TnA_GC Cuts at: 2144 2234 3889 2144 Size: 90 1655 4727 Fragments arranged by size: 4727 1655 90 Bme1580I G_kGCm'C Cuts at: 1790 3015 3047 3322 3528 4533 5689 1790 Size: 1225 32 275 206 1005 1156 2573 Fragments arranged by size: 2573 1225 1156 1005 275 206 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4203 5161 442 Size: 416 53 83 399 24 2786 958 1753 Fragments arranged by size: 2786 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2423 2738 2921 3407 3497 5132 1239 Size: 851 333 315 183 486 90 1635 2579 Fragments arranged by size: 2579 1635 851 486 333 315 183 90 Bpu10I CC'TnA_GC Cuts at: 2462 2462 Size: 6472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 2801 4597 5462 5760 2027 Size: 774 1796 865 298 2739 Fragments arranged by size: 2739 1796 865 774 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2759 3548 5135 882 Size: 617 1260 789 1587 2219 Fragments arranged by size: 2219 1587 1260 789 617 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2194 4279 4661 5296 Size: 53 83 186 936 661 2085 382 635 Cuts at: 5296 275 Size: 1451 Fragments arranged by size: 2085 1451 936 661 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3064 4905 5303 5577 5724 6135 3064 Size: 1841 398 274 147 411 3401 Fragments arranged by size: 3401 1841 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3545 3575 1178 Size: 30 165 30 2142 30 4075 Fragments arranged by size: 4075 2142 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3203 3236 3263 1186 Size: 2017 33 27 4395 Fragments arranged by size: 4395 2017 33 27 BseYI C'CCAG_C Cuts at: 2394 3370 3741 5675 6171 2394 Size: 976 371 1934 496 2695 Fragments arranged by size: 2695 1934 976 496 371 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2374 2894 3621 1163 Size: 573 638 520 727 4014 Fragments arranged by size: 4014 727 638 573 520 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3922 4683 5711 1540 Size: 275 186 1921 761 1028 2301 Fragments arranged by size: 2301 1921 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 3339 3722 4533 4618 5689 2013 Size: 1326 383 811 85 1071 2796 Fragments arranged by size: 2796 1326 1071 811 383 85 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 3015 3047 3322 3339 3528 3722 Size: 399 223 1002 32 275 17 189 194 Cuts at: 3722 3791 4533 4618 5689 1391 Size: 69 742 85 1071 2174 Fragments arranged by size: 2174 1071 1002 742 399 275 223 194 189 85 69 32 17 BspEI T'CCGG_A Cuts at: 3064 6135 3064 Size: 3071 3401 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2916 3643 3827 844 Size: 2072 727 184 3489 Fragments arranged by size: 3489 2072 727 184 BsrBI CCG'CTC Cuts at: 1394 1823 2179 3199 3255 4359 1394 Size: 429 356 1020 56 1104 3507 Fragments arranged by size: 3507 1104 1020 429 356 56 BsrDI GCAATG_nn' Cuts at: 65 1248 4967 5141 65 Size: 1183 3719 174 1396 Fragments arranged by size: 3719 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5116 5303 1700 Size: 110 3306 187 2869 Fragments arranged by size: 3306 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2928 96 Size: 2832 3640 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 2221 2453 4532 5544 2221 Size: 232 2079 1012 3149 Fragments arranged by size: 3149 2079 1012 232 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6472 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstYI r'GATC_y Cuts at: 1972 2712 2886 3802 4169 4554 4571 6023 6109 Size: 740 174 916 367 385 17 1452 86 Cuts at: 6109 6121 6467 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 916 740 385 367 346 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2361 2361 Size: 6472 BtgI C'CryG_G Cuts at: 513 1065 2004 2691 2968 3048 513 Size: 552 939 687 277 80 3937 Fragments arranged by size: 3937 939 687 552 277 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5733 Fragments arranged by size: 5733 683 56 BtsI GCAGTG_nn' Cuts at: 2525 3322 3526 3988 4782 4802 2525 Size: 797 204 462 794 20 4195 Fragments arranged by size: 4195 797 794 462 204 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3672 5479 809 Size: 507 2356 1807 1802 Fragments arranged by size: 2356 1807 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2265 2971 3919 Size: 54 1425 50 275 18 435 706 948 Cuts at: 3919 4808 6437 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 948 889 706 435 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3919 1537 Size: 275 2107 4090 Fragments arranged by size: 4090 2107 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3674 3710 4402 1353 Size: 774 1547 36 692 3423 Fragments arranged by size: 3423 1547 774 692 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5059 5433 5579 387 Size: 1064 138 3470 374 146 1280 Fragments arranged by size: 3470 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 3720 2011 Size: 1709 4763 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2423 2545 2738 2921 2960 3407 Size: 393 458 333 122 193 183 39 447 Cuts at: 3407 3497 3655 4535 5132 5919 6187 1239 Size: 90 158 880 597 787 268 1524 Fragments arranged by size: 1524 880 787 597 458 447 393 333 268 193 183 158 122 90 39 EcoNI CCTnn'n_nnAGG Cuts at: 2429 3278 2429 Size: 849 5623 EcoO109I rG'GnC_Cy Cuts at: 2345 2365 2411 2905 2935 3214 3283 3751 2345 Size: 20 46 494 30 279 69 468 5066 Fragments arranged by size: 5066 494 468 279 69 46 30 20 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3376 3376 Size: 6472 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 2197 2699 3116 3238 5619 1678 Size: 519 502 417 122 2381 2531 Fragments arranged by size: 2531 2381 519 502 417 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5113 5145 5187 5219 1373 Size: 32 135 32 3541 32 42 32 2626 Fragments arranged by size: 3541 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3816 4062 669 Size: 988 281 1878 246 3079 Fragments arranged by size: 3079 1878 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KasI G'GCGC_C Cuts at: 2193 2193 Size: 6472 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5586 5770 1305 Size: 4281 184 2007 Fragments arranged by size: 4281 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4650 Fragments arranged by size: 4650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4430 4789 4948 518 Size: 648 3264 359 159 2042 Fragments arranged by size: 3264 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2241 2377 2398 3888 4565 5713 5958 1606 Size: 635 136 21 1490 677 1148 245 2120 Fragments arranged by size: 2120 1490 1148 677 635 245 136 21 NarI GG'CG_CC Cuts at: 2194 2194 Size: 6472 NcoI C'CATG_G Cuts at: 513 1065 2004 2691 513 Size: 552 939 687 4294 Fragments arranged by size: 4294 939 687 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 2307 3011 3385 3830 5375 1132 Size: 97 1078 704 374 445 1545 2229 Fragments arranged by size: 2229 1545 1078 704 445 374 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6472 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 3534 3765 1108 Size: 1293 1133 231 3815 Fragments arranged by size: 3815 1293 1133 231 PfoI T'CCnGG_A Cuts at: 2253 2430 2715 3567 2253 Size: 177 285 852 5158 Fragments arranged by size: 5158 852 285 177 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 6054 6086 4541 Size: 32 1481 32 4927 Fragments arranged by size: 4927 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2345 2365 2411 2905 2935 3214 3283 2345 Size: 20 46 494 30 279 69 5534 Fragments arranged by size: 5534 494 279 69 46 30 20 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PspXI vC'TCGA_Gb Cuts at: 2150 2150 Size: 6472 PstI C_TGCA'G Cuts at: 830 2520 3663 3703 3824 830 Size: 1690 1143 40 121 3478 Fragments arranged by size: 3478 1690 1143 121 40 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2377 2377 Size: 6472 SacI G_AGCT'C Cuts at: 2013 3722 2013 Size: 1709 4763 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SanDI GG'GwC_CC Cuts at: 2411 2411 Size: 6472 SapI GCTCTTCn'nnn_ Cuts at: 2127 3710 2127 Size: 1583 4889 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2516 3659 3699 3820 4955 5636 5827 Size: 219 1471 1143 40 121 1135 681 191 Cuts at: 5827 826 Size: 1471 Fragments arranged by size: 1471 1471 1143 1135 681 219 191 121 40 SfoI GGC'GCC Cuts at: 2195 2195 Size: 6472 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 2051 3799 1872 Size: 179 1748 4545 Fragments arranged by size: 4545 1748 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 2042 2150 2816 4576 5477 Size: 197 926 69 30 108 666 1760 901 Cuts at: 5477 5739 820 Size: 262 1553 Fragments arranged by size: 1760 1553 926 901 666 262 197 108 69 30 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3385 3830 1132 Size: 2253 445 3774 Fragments arranged by size: 3774 2253 445 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2004 2691 2769 3911 513 Size: 552 939 687 78 1142 3074 Fragments arranged by size: 3074 1142 939 687 552 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2408 3572 4499 4516 4669 4854 5299 Size: 1255 828 1164 927 17 153 185 445 Cuts at: 5299 325 Size: 1498 Fragments arranged by size: 1498 1255 1164 927 828 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2928 4718 6430 Size: 274 80 33 51 1443 951 1790 1712 Cuts at: 6430 96 Size: 138 Fragments arranged by size: 1790 1712 1443 951 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2290 2322 2765 2797 2988 3020 3545 3577 6432 Size: 32 443 32 191 32 525 32 2855 Cuts at: 6432 6464 2290 Size: 32 2298 Fragments arranged by size: 2855 2298 525 443 191 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6472 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3615 3615 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 2012 2150 1943 Size: 69 138 6265 Fragments arranged by size: 6265 138 69 XmaI C'CCGG_G Cuts at: 1870 2049 3797 1870 Size: 179 1748 4545 Fragments arranged by size: 4545 1748 179 XmnI GAAnn'nnTTC Cuts at: 4601 4601 Size: 6472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4280 276 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SrfI SwaI