Restriction map for
pFN21AB6934
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 437 GTAGAC GT^[AC][GT]AC
AclI 485 AACGTT AA^CGTT
AcsI 550 GAATTT [GA]^AATT[CT]
AfaI 178 GTAC GT^AC
AflIII 174 ACACGT A^C[GA][CT]GT
ApoI 550 GAATTT [GA]^AATT[CT]
AspHI 633 GAGCAC G[AT]GC[AT]^C
BbvI 128 GCAGC GCAGC(8/12)
BmyI 633 GAGCAC G[AGT]GC[ACT]^C
BsaAI 175 CACGTA [CT]AC^GT[GA]
BsgI 34 CTGCAC GTGCAG(16/14)
BsiEI 86 CGGCCG CG[GA][CT]^CG
BsiHKAI 633 GAGCAC G[AT]GC[AT]^C
BsiYI 82 CCGCCGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 82 CCGCCGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 633 GAGCAC G[AGT]GC[ACT]^C
BssKI 104 CCTGG ^CC[ACGT]GG
BstAPI 69 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 104 CCTGG CC^[AT]GG
DpnII 309 GATC ^GATC
EaeI 86 CGGCCG [CT]^GGCC[GA]
EagI 86 CGGCCG C^GGCCG
EarI 577 CTCTTC CTCTTC(1/4)
EclXI 86 CGGCCG C^GGCCG
Eco52I 86 CGGCCG C^GGCCG
EcoRII 104 CCTGG ^CC[AT]GG
EcoT14I 458 CCAAGG C^C[AT][AT]GG
FauI 81 CCCGC CCCGC(4/6)
FseI 87 GGCCGGCC GGCCGG^CC
Ksp632I 577 CTCTTC CTCTTC(1/4)
MaeIII 278 GTGAC ^GT[ACGT]AC
MboI 309 GATC ^GATC
MseI 499 TTAA T^TAA
MvaI 104 CCTGG CC^[AT]GG
NdeII 309 GATC ^GATC
Psp1406I 485 AACGTT AA^CGTT
RsaI 178 GTAC GT^AC
Sau3AI 309 GATC ^GATC
ScrFI 104 CCTGG CC^[ACGT]GG
StyI 458 CCAAGG C^C[AT][AT]GG
Tru9I 499 TTAA T^TAA
TseI 128 GCAGC G^C[AT]GC
Tsp45I 278 GTGAC ^GT[GC]AC
Tsp509I 551 AATT ^AATT
TspRI 274 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AluI 113 AGCT AG^CT
349 AGCT
AlwNI 70 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
99 CAGTTCCTG
Asp700I 445 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
558 GAAGGTGTTC
AvaII 342 GGACC G^G[AT]CC
431 GGTCC
BfaI 13 CTAG C^TAG
607 CTAG
BsaJI 104 CCTGGG C^C[ACGT][ACGT]GG
458 CCAAGG
BsmAI 206 GAGAC GTCTC(1/5)
72 GTCTC
BsmFI 341 GGGAC GGGAC(10/14)
6 GTCCC
BsrFI 84 GCCGGC [GA]^CCGG[CT]
88 GCCGGC
Cfr10I 84 GCCGGC [GA]^CCGG[CT]
88 GCCGGC
Cfr13I 342 GGACC G^G[ACGT]CC
431 GGTCC
DdeI 353 CTCAG C^T[ACGT]AG
631 CTGAG
HaeIII 87 GGCC GG^CC
91 GGCC
HapII 85 CCGG C^CGG
89 CCGG
HinfI 219 GATTC G^A[ACGT]TC
241 GAATC
HpaII 85 CCGG C^CGG
89 CCGG
MaeI 13 CTAG C^TAG
607 CTAG
MaeII 176 ACGT A^CGT
486 ACGT
MspI 85 CCGG C^CGG
89 CCGG
NaeI 84 GCCGGC GCC^GGC
88 GCCGGC
NgoMIV 84 GCCGGC G^CCGGC
88 GCCGGC
NlaIV 341 GGGACC GG[ACGT]^[ACGT]CC
517 GGAGCC
Sau96I 342 GGACC G^G[ACGT]CC
431 GGTCC
SfaNI 229 GATGC GCATC(5/9)
611 GCATC
TaqI 17 TCGA T^CGA
392 TCGA
TfiI 219 GATTC G^A[AT]TC
241 GAATC
TthHB8I 17 TCGA T^CGA
392 TCGA
XmnI 445 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
558 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AciI BsrDI BsrI BstF5I Cac8I
Fnu4HI FokI ItaI MboII MnlI
MwoI NlaIII