Restriction map for
pFN21AB0413
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BbrPI BbsI BbvCI
BciVI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV FseI FspI
HincII HindII HindIII HpaI HphI
KpnI MamI MfeI MluI MroI
MunI NdeI NotI NruI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PstI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1027 GTCTAC GT^[AC][GT]AC
AcsI 575 AAATTT [GA]^AATT[CT]
ApaI 1281 GGGCCC GGGCC^C
ApoI 575 AAATTT [GA]^AATT[CT]
AvaI 885 CTCGGG C^[CT]CG[GA]G
BalI 45 TGGCCA TGG^CCA
BamHI 426 GGATCC G^GATCC
BanII 1281 GGGCCC G[GA]GC[CT]^C
BclI 437 TGATCA T^GATCA
BfaI 91 CTAG C^TAG
BsaI 240 GGTCTC GGTCTC(1/5)
BseRI 1225 GAGGAG GAGGAG(10/8)
BsmI 518 GCATTC GAATGC(1/-1)
BsoBI 885 CTCGGG C^[CT]CG[GA]G
BstYI 426 GGATCC [GA]^GATC[CT]
EagI 168 CGGCCG C^GGCCG
EarI 223 CTCTTC CTCTTC(1/4)
EclXI 168 CGGCCG C^GGCCG
Eco52I 168 CGGCCG C^GGCCG
FbaI 437 TGATCA T^GATCA
HinfI 648 GATTC G^A[ACGT]TC
Ksp632I 223 CTCTTC CTCTTC(1/4)
MaeI 91 CTAG C^TAG
MaeII 349 ACGT A^CGT
MflI 426 GGATCC [GA]^GATC[CT]
MluNI 45 TGGCCA TGG^CCA
MscI 45 TGGCCA TGG^CCA
MseI 995 TTAA T^TAA
NaeI 1184 GCCGGC GCC^GGC
NcoI 944 CCATGG C^CATGG
NgoMIV 1184 GCCGGC G^CCGGC
NheI 90 GCTAGC G^CTAGC
NspI 566 ACATGC [GA]CATG^[CT]
PvuI 717 CGATCG CGAT^CG
PvuII 704 CAGCTG CAG^CTG
SexAI 1013 ACCAGGT A^CC[AT]GGT
SfcI 1140 CTACAG C^T[GA][CT]AG
StuI 623 AGGCCT AGG^CCT
TfiI 648 GATTC G^A[AT]TC
Tru9I 995 TTAA T^TAA
Tsp509I 576 AATT ^AATT
XhoII 426 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 138 GTAC GT^AC
351 GTAC
AvaII 153 GGACC G^G[AT]CC
1193 GGACC
BbeI 80 GGCGCC GGCGC^C
202 GGCGCC
BcgI 177 CGACGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
318 GCATGGAGGTCG
BglI 616 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1370 GCCGCGTGGGC
BsgI 103 CTGCAC GTGCAG(16/14)
677 GTGCAG
BsiEI 168 CGGCCG CG[GA][CT]^CG
717 CGATCG
BsrFI 134 GCCGGT [GA]^CCGG[CT]
1184 GCCGGC
Cfr10I 134 GCCGGT [GA]^CCGG[CT]
1184 GCCGGC
DdeI 629 CTGAG C^T[ACGT]AG
1200 CTCAG
EcoT22I 316 ATGCAT ATGCA^T
568 ATGCAT
FauI 111 GCGGG CCCGC(4/6)
127 GCGGG
KasI 80 GGCGCC G^GCGCC
202 GGCGCC
KspI 165 CCGCGG CCGC^GG
810 CCGCGG
NarI 80 GGCGCC GG^CGCC
202 GGCGCC
NsiI 316 ATGCAT ATGCA^T
568 ATGCAT
PflMI 732 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1271 CCAACTCCTGG
PpuMI 152 AGGACCT [GA]G^G[AT]CC[CT]
1192 GGGACCC
PshAI 750 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1387 GACATGGGTC
RsaI 138 GTAC GT^AC
351 GTAC
SacII 165 CCGCGG CCGC^GG
810 CCGCGG
SfoI 80 GGCGCC GGC^GCC
202 GGCGCC
Van91I 732 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1271 CCAACTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI BanI BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RcaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI