(Circular) (Six-base) MAPSORT of: pFN21AB0413.seq Check: 6645 from: 1 to: 6106 pFN21AB0413 6106 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2121 2390 2121 Size: 269 5837 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3033 3449 1937 Size: 1096 416 4594 Fragments arranged by size: 4594 1096 416 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4169 5553 5821 1632 Size: 2537 1384 268 1917 Fragments arranged by size: 2537 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 3158 1166 Size: 1992 4114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 2161 2711 2779 3849 5421 2161 Size: 550 68 1070 1572 2846 Fragments arranged by size: 2846 1572 1070 550 68 ApaI G_GGCC'C Cuts at: 3290 3290 Size: 6106 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 2580 3415 3641 3793 5902 1493 Size: 1087 835 226 152 2109 1697 Fragments arranged by size: 2109 1697 1087 835 226 152 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2890 3431 1870 Size: 73 947 541 4545 Fragments arranged by size: 4545 947 541 73 BamHI G'GATC_C Cuts at: 2431 3436 3803 2431 Size: 1005 367 4734 Fragments arranged by size: 4734 1005 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2085 2207 2540 2834 Size: 325 217 627 279 19 122 333 294 Cuts at: 2834 3427 4882 618 Size: 593 1455 1842 Fragments arranged by size: 1842 1455 627 593 333 325 294 279 217 122 19 BanII G_rGCy'C Cuts at: 1391 3290 3425 1391 Size: 1899 135 4072 Fragments arranged by size: 4072 1899 135 BbeI G_GCGC'C Cuts at: 2089 2211 2089 Size: 122 5984 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2171 2205 2312 2346 4297 4331 1903 Size: 34 234 34 107 34 1951 34 3678 Fragments arranged by size: 3678 1951 234 107 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 2442 1548 Size: 894 5212 BfrBI ATG'CAT Cuts at: 2323 2575 2323 Size: 252 5854 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2627 3381 4717 Size: 107 122 71 631 605 955 754 1336 Cuts at: 4717 136 Size: 1525 Fragments arranged by size: 1525 1336 955 754 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 2071 2194 2543 3290 4167 5323 1790 Size: 281 123 349 747 877 1156 2573 Fragments arranged by size: 2573 1156 877 747 349 281 123 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2030 2368 3837 Size: 416 53 83 399 24 613 338 1469 Cuts at: 3837 4795 442 Size: 958 1753 Fragments arranged by size: 1753 1469 958 613 416 399 338 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2099 1056 Size: 1043 5063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4766 1239 Size: 3527 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4231 5096 5394 4231 Size: 865 298 4943 Fragments arranged by size: 4943 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2251 4769 882 Size: 617 752 2518 2219 Fragments arranged by size: 2518 2219 752 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2086 2208 2756 2824 Size: 53 83 186 936 553 122 548 68 Cuts at: 2824 3330 3366 3913 4295 4930 275 Size: 506 36 547 382 635 1451 Fragments arranged by size: 1451 936 635 553 548 547 506 382 186 122 83 68 53 36 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5881 Fragments arranged by size: 5881 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3245 1186 Size: 2059 4047 BseYI C'CCAG_C Cuts at: 2070 2480 3289 3361 5309 5805 2070 Size: 410 809 72 1948 496 2371 Fragments arranged by size: 2371 1948 809 496 410 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 2703 1163 Size: 573 357 610 4566 Fragments arranged by size: 4566 610 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2176 2725 3556 4317 5345 1540 Size: 275 186 175 549 831 761 1028 2301 Fragments arranged by size: 2301 1028 831 761 549 275 186 175 BsiHKAI G_wGCw'C Cuts at: 4167 4252 5323 4167 Size: 85 1071 4950 Fragments arranged by size: 4950 1071 85 BsmI GAATG_Cn' Cuts at: 2523 3617 3710 2523 Size: 1094 93 4919 Fragments arranged by size: 4919 1094 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2831 2898 3227 2831 Size: 67 329 5710 Fragments arranged by size: 5710 329 67 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2194 2543 3290 3425 4167 4252 Size: 399 281 123 349 747 135 742 85 Cuts at: 4252 5323 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 747 742 399 349 281 135 123 85 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 2565 2656 3063 3995 5725 2565 Size: 91 407 932 1730 2946 Fragments arranged by size: 2946 1730 932 407 91 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2390 2787 3461 844 Size: 1277 269 397 674 3489 Fragments arranged by size: 3489 1277 674 397 269 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4601 4775 65 Size: 1183 3353 174 1396 Fragments arranged by size: 3353 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2139 3189 4750 4937 1700 Size: 110 329 1050 1561 187 2869 Fragments arranged by size: 2869 1561 1050 329 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6106 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstYI r'GATC_y Cuts at: 1972 2431 3436 3803 4188 4205 5657 5743 5755 Size: 459 1005 367 385 17 1452 86 12 Cuts at: 5755 6101 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1005 459 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2170 2815 2949 513 Size: 552 1105 645 134 3670 Fragments arranged by size: 3670 1105 645 552 134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2026 487 Size: 683 56 800 4567 Fragments arranged by size: 4567 800 683 56 BtsI GCAGTG_nn' Cuts at: 2405 2411 2875 2973 2980 3622 4416 4436 2405 Size: 6 464 98 7 642 794 20 4075 Fragments arranged by size: 4075 794 642 464 98 20 7 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2173 3273 Size: 54 1425 50 275 18 220 123 1100 Cuts at: 3273 3553 4442 6071 8 Size: 280 889 1629 43 Fragments arranged by size: 1629 1425 1100 889 280 275 220 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2173 3553 1537 Size: 275 361 1380 4090 Fragments arranged by size: 4090 1380 361 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2234 4036 1353 Size: 881 1802 3423 Fragments arranged by size: 3423 1802 881 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2537 4693 5067 5213 387 Size: 1064 138 948 2156 374 146 1280 Fragments arranged by size: 2156 1280 1064 948 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4169 4766 5553 5821 1239 Size: 393 2537 597 787 268 1524 Fragments arranged by size: 2537 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2516 3104 3131 2516 Size: 588 27 5491 Fragments arranged by size: 5491 588 27 EcoO109I rG'GnC_Cy Cuts at: 2158 3198 3286 2158 Size: 1040 88 4978 Fragments arranged by size: 4978 1040 88 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2089 2211 2496 2968 5253 1678 Size: 411 122 285 472 2285 2531 Fragments arranged by size: 2531 2285 472 411 285 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2319 2351 2714 2746 4747 Size: 32 135 32 747 32 363 32 2001 Cuts at: 4747 4779 4821 4853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2001 747 363 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KasI G'GCGC_C Cuts at: 2085 2207 2085 Size: 122 5984 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 3072 5220 5404 1305 Size: 969 798 2148 184 2007 Fragments arranged by size: 2148 2007 969 798 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2052 10 Size: 54 1425 343 220 4064 Fragments arranged by size: 4064 1425 343 220 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 3107 3158 4064 4423 4582 518 Size: 648 1404 537 51 906 359 159 2042 Fragments arranged by size: 2042 1404 906 648 537 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2044 2172 2711 2817 3522 4199 5347 5592 Size: 438 128 539 106 705 677 1148 245 Cuts at: 5592 1606 Size: 2120 Fragments arranged by size: 2120 1148 705 677 539 438 245 128 106 NaeI GCC'GGC Cuts at: 3191 3191 Size: 6106 NarI GG'CG_CC Cuts at: 2086 2208 2086 Size: 122 5984 NcoI C'CATG_G Cuts at: 513 1065 2949 513 Size: 552 1884 3670 Fragments arranged by size: 3670 1884 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NgoMIV G'CCGG_C Cuts at: 3189 3189 Size: 6106 NheI G'CTAG_C Cuts at: 1052 2095 1052 Size: 1043 5063 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NsiI A_TGCA'T Cuts at: 2325 2577 2325 Size: 252 5854 NspI r_CATG'y Cuts at: 1132 1229 2575 3464 5009 1132 Size: 97 1346 889 1545 2229 Fragments arranged by size: 2229 1545 1346 889 97 PasI CC'CwG_GG Cuts at: 1348 2600 1348 Size: 1252 4854 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 2743 3282 1108 Size: 1635 539 3932 Fragments arranged by size: 3932 1635 539 PfoI T'CCnGG_A Cuts at: 2124 2124 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 3198 2158 Size: 1040 5066 PshAI GACnn'nnGTC Cuts at: 1613 2759 3396 1613 Size: 1146 637 4323 Fragments arranged by size: 4323 1146 637 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 3286 3286 Size: 6106 PstI C_TGCA'G Cuts at: 830 3458 830 Size: 2628 3478 PvuI CG_AT'CG Cuts at: 2001 2725 2001 Size: 724 5382 PvuII CAG'CTG Cuts at: 2711 2711 Size: 6106 SacII CC_GC'GG Cuts at: 2173 2818 2173 Size: 645 5461 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SanDI GG'GwC_CC Cuts at: 3198 3198 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SexAI A'CCwGG_T Cuts at: 3018 3018 Size: 6106 SfcI C'TryA_G Cuts at: 826 1045 3145 3454 4589 5270 5461 826 Size: 219 2100 309 1135 681 191 1471 Fragments arranged by size: 2100 1471 1135 681 309 219 191 SfoI GGC'GCC Cuts at: 2087 2209 2087 Size: 122 5984 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4210 5111 5373 820 Size: 197 926 2267 901 262 1553 Fragments arranged by size: 2267 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 2630 1517 Size: 1113 4993 StyI C'CwwG_G Cuts at: 513 1065 2311 2671 2949 3166 3545 513 Size: 552 1246 360 278 217 379 3074 Fragments arranged by size: 3074 1246 552 379 360 278 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 2551 2810 4133 4150 4303 4488 Size: 1255 474 497 259 1323 17 153 185 Cuts at: 4488 4933 325 Size: 445 1498 Fragments arranged by size: 1498 1323 1255 497 474 445 259 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4352 6064 96 Size: 274 80 33 51 1443 2375 1712 138 Fragments arranged by size: 2375 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6066 6098 6066 Size: 32 6074 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2078 2465 2710 2078 Size: 387 245 5474 Fragments arranged by size: 5474 387 245 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI