Restriction map for
pFN21AB0413
(Linear) (Six-base) MAPSORT of: KIBB0413.seq Check: 4532 from: 1 to: 1401
KIBB0413.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 116 385 1401
Size: 116 269 1016
Fragments arranged by size:
1016 269 116
AccI GT'mk_AC
Cuts at: 0 1028 1401
Size: 1028 373
AleI CACnn'nnGTG
Cuts at: 0 1153 1401
Size: 1153 248
AlwNI CAG_nnn'CTG
Cuts at: 0 156 706 774 1401
Size: 156 550 68 627
Fragments arranged by size:
627 550 156 68
ApaI G_GGCC'C
Cuts at: 0 1285 1401
Size: 1285 116
ApoI r'AATT_y
Cuts at: 0 575 1401
Size: 575 826
AvaI C'yCGr_G
Cuts at: 0 885 1401
Size: 885 516
BamHI G'GATC_C
Cuts at: 0 426 1401
Size: 426 975
BanI G'GyrC_C
Cuts at: 0 61 80 202 535 829 1401
Size: 61 19 122 333 294 572
Fragments arranged by size:
572 333 294 122 61 19
BanII G_rGCy'C
Cuts at: 0 1285 1401
Size: 1285 116
BbeI G_GCGC'C
Cuts at: 0 84 206 1401
Size: 84 122 1195
Fragments arranged by size:
1195 122 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 200 307 341 1401
Size: 166 34 107 34 1060
Fragments arranged by size:
1060 166 107 34 34
BclI T'GATC_A
Cuts at: 0 437 1401
Size: 437 964
BfrBI ATG'CAT
Cuts at: 0 318 570 1401
Size: 318 252 831
Fragments arranged by size:
831 318 252
BglI GCCn_nnn'nGGC
Cuts at: 0 622 1376 1401
Size: 622 754 25
Fragments arranged by size:
754 622 25
Bme1580I G_kGCm'C
Cuts at: 0 66 189 538 1285 1401
Size: 66 123 349 747 116
Fragments arranged by size:
747 349 123 116 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 363 1401
Size: 25 338 1038
Fragments arranged by size:
1038 338 25
BmtI G_CTAG'C
Cuts at: 0 94 1401
Size: 94 1307
BsaI GGTCTCn'nnnn_
Cuts at: 0 246 1401
Size: 246 1155
BsaHI Gr'CG_yC
Cuts at: 0 81 203 751 819 1325 1361 1401
Size: 81 122 548 68 506 36 40
Fragments arranged by size:
548 506 122 81 68 40 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1240 1401
Size: 1240 161
BseYI C'CCAG_C
Cuts at: 0 65 475 1284 1356 1401
Size: 65 410 809 72 45
Fragments arranged by size:
809 410 72 65 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 698 1401
Size: 88 610 703
Fragments arranged by size:
703 610 88
BsiEI CG_ry'CG
Cuts at: 0 171 720 1401
Size: 171 549 681
Fragments arranged by size:
681 549 171
BsmI GAATG_Cn'
Cuts at: 0 518 1401
Size: 518 883
BsmBI CGTCTCn'nnnn_
Cuts at: 0 826 893 1222 1401
Size: 826 67 329 179
Fragments arranged by size:
826 329 179 67
Bsp1286I G_dGCh'C
Cuts at: 0 66 189 538 1285 1401
Size: 66 123 349 747 116
Fragments arranged by size:
747 349 123 116 66
BspHI T'CATG_A
Cuts at: 0 560 651 1058 1401
Size: 560 91 407 343
Fragments arranged by size:
560 407 343 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 385 782 1401
Size: 116 269 397 619
Fragments arranged by size:
619 397 269 116
BsrFI r'CCGG_y
Cuts at: 0 134 1184 1401
Size: 134 1050 217
Fragments arranged by size:
1050 217 134
BstYI r'GATC_y
Cuts at: 0 426 1401
Size: 426 975
BtgI C'CryG_G
Cuts at: 0 165 810 944 1401
Size: 165 645 134 457
Fragments arranged by size:
645 457 165 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 21 1401
Size: 21 1380
BtsI GCAGTG_nn'
Cuts at: 0 400 406 870 968 975 1401
Size: 400 6 464 98 7 426
Fragments arranged by size:
464 426 400 98 7 6
EaeI y'GGCC_r
Cuts at: 0 45 168 1268 1401
Size: 45 123 1100 133
Fragments arranged by size:
1100 133 123 45
EagI C'GGCC_G
Cuts at: 0 168 1401
Size: 168 1233
EarI CTCTTCn'nnn_
Cuts at: 0 229 1401
Size: 229 1172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 1401
Size: 532 869
EcoNI CCTnn'n_nnAGG
Cuts at: 0 511 1099 1126 1401
Size: 511 588 27 275
Fragments arranged by size:
588 511 275 27
EcoO109I rG'GnC_Cy
Cuts at: 0 153 1193 1281 1401
Size: 153 1040 88 120
Fragments arranged by size:
1040 153 120 88
HaeII r_GCGC'y
Cuts at: 0 84 206 491 963 1401
Size: 84 122 285 472 438
Fragments arranged by size:
472 438 285 122 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 709 741 1401
Size: 314 32 363 32 660
Fragments arranged by size:
660 363 314 32 32
KasI G'GCGC_C
Cuts at: 0 80 202 1401
Size: 80 122 1199
Fragments arranged by size:
1199 122 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1067 1401
Size: 269 798 334
Fragments arranged by size:
798 334 269
MscI TGG'CCA
Cuts at: 0 47 1401
Size: 47 1354
MslI CAynn'nnrTG
Cuts at: 0 565 1102 1153 1401
Size: 565 537 51 248
Fragments arranged by size:
565 537 248 51
MspA1I CmG'CkG
Cuts at: 0 39 167 706 812 1401
Size: 39 128 539 106 589
Fragments arranged by size:
589 539 128 106 39
NaeI GCC'GGC
Cuts at: 0 1186 1401
Size: 1186 215
NarI GG'CG_CC
Cuts at: 0 81 203 1401
Size: 81 122 1198
Fragments arranged by size:
1198 122 81
NcoI C'CATG_G
Cuts at: 0 944 1401
Size: 944 457
NgoMIV G'CCGG_C
Cuts at: 0 1184 1401
Size: 1184 217
NheI G'CTAG_C
Cuts at: 0 90 1401
Size: 90 1311
NsiI A_TGCA'T
Cuts at: 0 320 572 1401
Size: 320 252 829
Fragments arranged by size:
829 320 252
NspI r_CATG'y
Cuts at: 0 570 1401
Size: 570 831
PasI CC'CwG_GG
Cuts at: 0 595 1401
Size: 595 806
PflMI CCAn_nnn'nTGG
Cuts at: 0 738 1277 1401
Size: 738 539 124
Fragments arranged by size:
738 539 124
PfoI T'CCnGG_A
Cuts at: 0 119 1401
Size: 119 1282
PpuMI rG'GwC_Cy
Cuts at: 0 153 1193 1401
Size: 153 1040 208
Fragments arranged by size:
1040 208 153
PshAI GACnn'nnGTC
Cuts at: 0 754 1391 1401
Size: 754 637 10
Fragments arranged by size:
754 637 10
PspOMI G'GGCC_C
Cuts at: 0 1281 1401
Size: 1281 120
PvuI CG_AT'CG
Cuts at: 0 720 1401
Size: 720 681
PvuII CAG'CTG
Cuts at: 0 706 1401
Size: 706 695
SacII CC_GC'GG
Cuts at: 0 168 813 1401
Size: 168 645 588
Fragments arranged by size:
645 588 168
SanDI GG'GwC_CC
Cuts at: 0 1193 1401
Size: 1193 208
SexAI A'CCwGG_T
Cuts at: 0 1013 1401
Size: 1013 388
SfcI C'TryA_G
Cuts at: 0 1140 1401
Size: 1140 261
SfoI GGC'GCC
Cuts at: 0 82 204 1401
Size: 82 122 1197
Fragments arranged by size:
1197 122 82
StuI AGG'CCT
Cuts at: 0 625 1401
Size: 625 776
StyI C'CwwG_G
Cuts at: 0 306 666 944 1161 1401
Size: 306 360 278 217 240
Fragments arranged by size:
360 306 278 240 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 546 805 1401
Size: 49 497 259 596
Fragments arranged by size:
596 497 259 49
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 73 460 705 1401
Size: 73 387 245 696
Fragments arranged by size:
696 387 245 73
Enzymes that do cut:
AarI AccI AleI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BcgI BclI BfrBI BglI Bme1580I
BmrI BmtI BsaI BsaHI BseRI BseYI BsgI BsiEI
BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BstYI BtgI
BtgZI BtsI EaeI EagI EarI EciI EcoNI EcoO109I
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PvuI PvuII SacII SanDI
SexAI SfcI SfoI StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BciVI BglII BlpI BmgBI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EcoICRI Eco57MI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NotI NruI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PstI RsrII SacI SalI SapI
SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI