Restriction map for
pFN21AB0405
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FbaI
FseI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 805 GTCTAC GT^[AC][GT]AC
AccII 1226 CGCG CG^CG
AcsI 613 GAATTC [GA]^AATT[CT]
AhdI 466 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1336 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1378 CCTGAGG CC^T[ACGT]AGG
ApoI 613 GAATTC [GA]^AATT[CT]
AspEI 466 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 344 GAGCAC G[AT]GC[AT]^C
AspI 357 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 539 TGCGCA TGC^GCA
BbsI 649 GAAGAC GAAGAC(2/6)
BbvCI 309 CCTCAGC CCTCAGC(-5/-2)
BglI 1202 GCCGGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 649 GAAGAC GAAGAC(2/6)
BsaBI 972 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 344 GAGCAC G[AT]GC[AT]^C
BsmAI 1150 GAGAC GTCTC(1/5)
BsmI 119 GAATGC GAATGC(1/-1)
BsrDI 810 CATTGC GCAATG(2/0)
BsrFI 1046 GCCGGT [GA]^CCGG[CT]
BstEII 522 GGTCACC G^GT[ACGT]ACC
BstPI 522 GGTCACC G^GT[ACGT]ACC
BstUI 1226 CGCG CG^CG
Bsu36I 1378 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1046 GCCGGT [GA]^CCGG[CT]
DsaI 327 CCATGG C^C[GA][CT]GG
Eam1105I 466 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1378 CCTGAGG CC^T[ACGT]AGG
EcoO65I 522 GGTCACC G^GT[ACGT]ACC
EcoRI 613 GAATTC G^AATTC
FspI 539 TGCGCA TGC^GCA
HinfI 1099 GAATC G^A[ACGT]TC
MaeII 467 ACGT A^CGT
MaeIII 523 GTCAC ^GT[ACGT]AC
MamI 972 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1226 CGCG CG^CG
NcoI 327 CCATGG C^CATGG
NspI 417 GCATGT [GA]CATG^[CT]
PflFI 357 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1039 GAAGAGC GCTCTTC(1/4)
StuI 11 AGGCCT AGG^CCT
TfiI 1099 GAATC G^A[AT]TC
Tsp45I 523 GTCAC ^GT[GC]AC
Tth111I 357 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 1032 GTAC GT^AC
1332 GTAC
BalI 666 TGGCCA TGG^CCA
1078 TGGCCA
BpmI 339 CTCCAG CTGGAG(16/14)
180 CTGGAG
BseRI 1138 GAGGAG GAGGAG(10/8)
1256 GAGGAG
BsgI 1342 CTGCAC GTGCAG(16/14)
1190 GTGCAG
BsmFI 474 GTCCC GGGAC(10/14)
879 GTCCC
BstXI 321 CCAAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
596 CCAAGCAACTGG
BstYI 623 AGATCC [GA]^GATC[CT]
971 AGATCC
EarI 946 GAAGAG CTCTTC(1/4)
1039 GAAGAG
HincII 354 GTTGAC GT[CT]^[GA]AC
1238 GTCAAC
HindII 354 GTTGAC GT[CT]^[GA]AC
1238 GTCAAC
Ksp632I 946 GAAGAG CTCTTC(1/4)
1039 GAAGAG
MflI 623 AGATCC [GA]^GATC[CT]
971 AGATCC
MluNI 666 TGGCCA TGG^CCA
1078 TGGCCA
MscI 666 TGGCCA TGG^CCA
1078 TGGCCA
MslI 885 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1183 CACTGCAGTG
PflMI 33 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1347 CCACACTTTGG
PstI 427 CTGCAG CTGCA^G
1185 CTGCAG
RsaI 1032 GTAC GT^AC
1332 GTAC
SfaNI 459 GATGC GCATC(5/9)
977 GCATC
SfcI 427 CTGCAG C^T[GA][CT]AG
1185 CTGCAG
SmaI 515 CCCGGG CCC^GGG
1165 CCCGGG
Tsp509I 614 AATT ^AATT
1230 AATT
Van91I 33 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1347 CCACACTTTGG
XhoII 623 AGATCC [GA]^GATC[CT]
971 AGATCC
XmaCI 515 CCCGGG C^CCGGG
1165 CCCGGG
XmaI 515 CCCGGG C^CCGGG
1165 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI StyI TseI TspRI