(Circular) (Six-base) MAPSORT of: pFN21AB0405.seq Check: 9577 from: 1 to: 6100 pFN21AB0405 6100 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2811 3443 1937 Size: 874 632 4594 Fragments arranged by size: 4594 874 632 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 2564 4163 5547 5815 1632 Size: 617 315 1599 1384 268 1917 Fragments arranged by size: 1917 1599 1384 617 315 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 2476 4829 2476 Size: 2353 3747 AleI CACnn'nnGTG Cuts at: 1166 3192 1166 Size: 2026 4074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 3346 3843 5415 3346 Size: 497 1572 4031 Fragments arranged by size: 4031 1572 497 ApaI G_GGCC'C Cuts at: 2059 2303 2578 2831 3117 2059 Size: 244 275 253 286 5042 Fragments arranged by size: 5042 286 275 253 244 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 2618 3409 3635 3787 5896 1493 Size: 1125 791 226 152 2109 1697 Fragments arranged by size: 2109 1697 1125 791 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2520 3170 3258 3425 1870 Size: 73 577 650 88 167 4545 Fragments arranged by size: 4545 650 577 167 88 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3421 4876 618 Size: 325 217 627 1634 1455 1842 Fragments arranged by size: 1842 1634 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2059 2303 2450 2578 2831 3117 3152 3419 Size: 668 244 147 128 253 286 35 267 Cuts at: 3419 1391 Size: 4072 Fragments arranged by size: 4072 668 286 267 253 244 147 128 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2661 928 Size: 971 762 4367 Fragments arranged by size: 4367 971 762 BbvCI CC'TCA_GC Cuts at: 2315 2315 Size: 6100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3213 4711 136 Size: 107 122 71 631 605 1541 1498 1525 Fragments arranged by size: 1541 1525 1498 631 605 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 2059 2070 2303 2578 2743 2831 3005 3117 Size: 269 11 233 275 165 88 174 112 Cuts at: 3117 4161 5317 1790 Size: 1044 1156 2573 Fragments arranged by size: 2573 1156 1044 275 269 233 174 165 112 88 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3831 4789 442 Size: 416 53 83 399 24 2414 958 1753 Fragments arranged by size: 2414 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2307 2307 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2206 2329 4760 1239 Size: 967 123 2431 2579 Fragments arranged by size: 2579 2431 967 123 Bpu10I CC'TnA_GC Cuts at: 2315 2315 Size: 6100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4763 882 Size: 617 3264 2219 Fragments arranged by size: 3264 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 2981 6094 2981 Size: 3113 2987 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3907 4289 4924 275 Size: 53 83 186 936 2374 382 635 1451 Fragments arranged by size: 2374 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2377 2407 2852 2882 1178 Size: 30 165 30 974 30 445 30 4396 Fragments arranged by size: 4396 974 445 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3158 3276 1186 Size: 1972 118 4010 Fragments arranged by size: 4010 1972 118 BseYI C'CCAG_C Cuts at: 2131 2302 2388 2571 2594 3110 3392 5303 5799 Size: 171 86 183 23 516 282 1911 496 Cuts at: 5799 2131 Size: 2432 Fragments arranged by size: 2432 1911 516 496 282 183 171 86 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3216 3332 1163 Size: 573 1480 116 3931 Fragments arranged by size: 3931 1480 573 116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2353 4161 4246 5317 2353 Size: 1808 85 1071 3136 Fragments arranged by size: 3136 1808 1071 85 BsmI GAATG_Cn' Cuts at: 2130 3611 3704 2130 Size: 1481 93 4526 Fragments arranged by size: 4526 1481 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2070 2303 2353 2450 2578 2743 Size: 399 269 11 233 50 97 128 165 Cuts at: 2743 2831 3005 3117 3152 3419 4161 4246 5317 Size: 88 174 112 35 267 742 85 1071 Cuts at: 5317 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 269 267 233 174 165 128 112 97 88 85 50 35 11 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3455 844 Size: 2611 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2814 4595 4769 65 Size: 1183 1566 1781 174 1396 Fragments arranged by size: 1781 1566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3051 4744 4931 1700 Size: 110 1241 1693 187 2869 Fragments arranged by size: 2869 1693 1241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6100 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 2527 1184 Size: 1343 4757 BstXI CCAn_nnnn'nTGG Cuts at: 2333 2608 2333 Size: 275 5825 BstYI r'GATC_y Cuts at: 1972 2628 2976 3430 3797 4182 4199 5651 5737 Size: 656 348 454 367 385 17 1452 86 Cuts at: 5737 5749 6095 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 656 454 385 367 348 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3384 3384 Size: 6100 BtgI C'CryG_G Cuts at: 513 1065 2004 2332 513 Size: 552 939 328 4281 Fragments arranged by size: 4281 939 552 328 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3208 487 Size: 683 56 1982 3379 Fragments arranged by size: 3379 1982 683 56 BtsI GCAGTG_nn' Cuts at: 2547 3187 3199 3616 4410 4430 2547 Size: 640 12 417 794 20 4217 Fragments arranged by size: 4217 794 640 417 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3217 3252 529 Size: 35 2653 35 3377 Fragments arranged by size: 3377 2653 35 35 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2671 3083 3205 Size: 54 1425 50 275 18 841 412 122 Cuts at: 3205 3547 4436 6065 8 Size: 342 889 1629 43 Fragments arranged by size: 1629 1425 889 841 412 342 275 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2946 3039 4030 1353 Size: 1593 93 991 3423 Fragments arranged by size: 3423 1593 991 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2178 2385 4687 5061 5207 387 Size: 1064 138 589 207 2302 374 146 1280 Fragments arranged by size: 2302 1280 1064 589 374 207 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2249 2329 2564 4163 4760 5547 Size: 393 574 43 80 235 1599 597 787 Cuts at: 5547 5815 1239 Size: 268 1524 Fragments arranged by size: 1599 1524 787 597 574 393 268 235 80 43 EcoO109I rG'GnC_Cy Cuts at: 2048 2056 2575 2827 2828 3114 2048 Size: 8 519 252 1 286 5034 Fragments arranged by size: 5034 519 286 252 8 1 EcoRI G'AATT_C Cuts at: 2618 3409 3787 2618 Size: 791 378 4931 Fragments arranged by size: 4931 791 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2719 2719 Size: 6100 FspI TGC'GCA Cuts at: 2546 4606 2546 Size: 2060 4040 HaeII r_GCGC'y Cuts at: 1678 2344 2587 3052 5247 1678 Size: 666 243 465 2195 2531 Fragments arranged by size: 2531 2195 666 465 243 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2377 2409 2462 2494 2751 Size: 32 135 32 805 32 53 32 257 Cuts at: 2751 2783 2867 2899 3038 3070 4741 4773 4815 Size: 32 84 32 139 32 1671 32 42 Cuts at: 4815 4847 1373 Size: 32 2626 Fragments arranged by size: 2626 1671 805 257 139 135 84 53 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2361 3245 3444 3690 669 Size: 988 281 423 884 199 246 3079 Fragments arranged by size: 3079 988 884 423 281 246 199 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2829 5214 5398 1305 Size: 1524 2385 184 2007 Fragments arranged by size: 2385 2007 1524 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2673 3085 10 Size: 54 1425 343 841 412 3025 Fragments arranged by size: 3025 1425 841 412 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2894 3192 4058 4417 4576 518 Size: 648 1728 298 866 359 159 2042 Fragments arranged by size: 2042 1728 866 648 359 298 159 MspA1I CmG'CkG Cuts at: 1606 2101 2131 2232 2260 2571 3110 3250 3379 Size: 495 30 101 28 311 539 140 129 Cuts at: 3379 3516 4193 5341 5586 1606 Size: 137 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 539 495 311 245 140 137 129 101 30 28 NcoI C'CATG_G Cuts at: 513 1065 2004 2332 513 Size: 552 939 328 4281 Fragments arranged by size: 4281 939 552 328 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2426 3458 5003 1132 Size: 97 1197 1032 1545 2229 Fragments arranged by size: 2229 1545 1197 1032 97 PasI CC'CwG_GG Cuts at: 1348 2038 2296 2482 2824 1348 Size: 690 258 186 342 4624 Fragments arranged by size: 4624 690 342 258 186 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2044 3358 1108 Size: 936 1314 3850 Fragments arranged by size: 3850 1314 936 PfoI T'CCnGG_A Cuts at: 2183 2985 2183 Size: 802 5298 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 2055 2299 2574 2827 3113 2055 Size: 244 275 253 286 5042 Fragments arranged by size: 5042 286 275 253 244 PstI C_TGCA'G Cuts at: 830 2436 3194 3452 830 Size: 1606 758 258 3478 Fragments arranged by size: 3478 1606 758 258 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2131 2232 2260 3110 3250 3379 2131 Size: 101 28 850 140 129 4852 Fragments arranged by size: 4852 850 140 129 101 28 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SapI GCTCTTCn'nnn_ Cuts at: 3039 3039 Size: 6100 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2432 3190 3448 4583 5264 5455 826 Size: 219 1387 758 258 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 758 681 258 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 2522 3172 3427 1872 Size: 650 650 255 4545 Fragments arranged by size: 4545 650 650 255 SmlI C'TyrA_G Cuts at: 820 1017 1943 4204 5105 5367 820 Size: 197 926 2261 901 262 1553 Fragments arranged by size: 2261 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 2018 1517 Size: 501 5599 StyI C'CwwG_G Cuts at: 513 1065 2004 2051 2332 2682 3539 513 Size: 552 939 47 281 350 857 3074 Fragments arranged by size: 3074 939 857 552 350 281 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2134 2228 2466 2608 2703 2837 Size: 1255 465 89 94 238 142 95 134 Cuts at: 2837 4127 4144 4297 4482 4927 325 Size: 1290 17 153 185 445 1498 Fragments arranged by size: 1498 1290 1255 465 445 238 185 153 142 134 95 94 89 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4346 6058 96 Size: 274 80 33 51 1443 2369 1712 138 Fragments arranged by size: 2369 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2852 2884 6060 6092 2852 Size: 32 3176 32 2860 Fragments arranged by size: 3176 2860 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2365 1316 Size: 1049 5051 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 2520 3170 3425 1870 Size: 650 650 255 4545 Fragments arranged by size: 4545 650 650 255 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI