Restriction map for
pFN21AB0405
(Linear) (Six-base) MAPSORT of: KIBB0405.seq Check: 5050 from: 1 to: 1395
KIBB0405.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 0 806 1395
Size: 806 589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 559 1395
Size: 244 315 836
Fragments arranged by size:
836 315 244
AhdI GACnn_n'nnGTC
Cuts at: 0 471 1395
Size: 471 924
AleI CACnn'nnGTG
Cuts at: 0 1187 1395
Size: 1187 208
AlwNI CAG_nnn'CTG
Cuts at: 0 1341 1395
Size: 1341 54
ApaI G_GGCC'C
Cuts at: 0 54 298 573 826 1112 1395
Size: 54 244 275 253 286 283
Fragments arranged by size:
286 283 275 253 244 54
ApoI r'AATT_y
Cuts at: 0 613 1395
Size: 613 782
AvaI C'yCGr_G
Cuts at: 0 515 1165 1253 1395
Size: 515 650 88 142
Fragments arranged by size:
650 515 142 88
BanII G_rGCy'C
Cuts at: 0 54 298 445 573 826 1112 1147 1395
Size: 54 244 147 128 253 286 35 248
Fragments arranged by size:
286 253 248 244 147 128 54 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 656 1395
Size: 656 739
BbvCI CC'TCA_GC
Cuts at: 0 310 1395
Size: 310 1085
BglI GCCn_nnn'nGGC
Cuts at: 0 1208 1395
Size: 1208 187
Bme1580I G_kGCm'C
Cuts at: 0 54 65 298 573 738 826 1000 1112
Size: 54 11 233 275 165 88 174 112
Cuts at: 1112 1395
Size: 283
Fragments arranged by size:
283 275 233 174 165 112 88 54
11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 302 1395
Size: 302 1093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 324 1395
Size: 201 123 1071
Fragments arranged by size:
1071 201 123
Bpu10I CC'TnA_GC
Cuts at: 0 310 1395
Size: 310 1085
BsaBI GATnn'nnATC
Cuts at: 0 976 1395
Size: 976 419
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 372 402 847 877 1395
Size: 372 30 445 30 518
Fragments arranged by size:
518 445 372 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1153 1271 1395
Size: 1153 118 124
Fragments arranged by size:
1153 124 118
BseYI C'CCAG_C
Cuts at: 0 126 297 383 566 589 1105 1387 1395
Size: 126 171 86 183 23 516 282 8
Fragments arranged by size:
516 282 183 171 126 86 23 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1211 1327 1395
Size: 1211 116 68
Fragments arranged by size:
1211 116 68
BsiHKAI G_wGCw'C
Cuts at: 0 348 1395
Size: 348 1047
BsmI GAATG_Cn'
Cuts at: 0 125 1395
Size: 125 1270
Bsp1286I G_dGCh'C
Cuts at: 0 54 65 298 348 445 573 738 826
Size: 54 11 233 50 97 128 165 88
Cuts at: 826 1000 1112 1147 1395
Size: 174 112 35 248
Fragments arranged by size:
248 233 174 165 128 112 97 88
54 50 35 11
BsrDI GCAATG_nn'
Cuts at: 0 809 1395
Size: 809 586
BsrFI r'CCGG_y
Cuts at: 0 1046 1395
Size: 1046 349
BstEII G'GTnAC_C
Cuts at: 0 522 1395
Size: 522 873
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 603 1395
Size: 328 275 792
Fragments arranged by size:
792 328 275
BstYI r'GATC_y
Cuts at: 0 623 971 1395
Size: 623 348 424
Fragments arranged by size:
623 424 348
Bsu36I CC'TnA_GG
Cuts at: 0 1379 1395
Size: 1379 16
BtgI C'CryG_G
Cuts at: 0 327 1395
Size: 327 1068
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1203 1395
Size: 1203 192
BtsI GCAGTG_nn'
Cuts at: 0 542 1182 1194 1395
Size: 542 640 12 201
Fragments arranged by size:
640 542 201 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1212 1247 1395
Size: 1212 35 148
Fragments arranged by size:
1212 148 35
EaeI y'GGCC_r
Cuts at: 0 666 1078 1200 1395
Size: 666 412 122 195
Fragments arranged by size:
666 412 195 122
EarI CTCTTCn'nnn_
Cuts at: 0 941 1034 1395
Size: 941 93 361
Fragments arranged by size:
941 361 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 173 380 1395
Size: 173 207 1015
Fragments arranged by size:
1015 207 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 244 324 559 1395
Size: 201 43 80 235 836
Fragments arranged by size:
836 235 201 80 43
EcoO109I rG'GnC_Cy
Cuts at: 0 43 51 570 822 823 1109 1395
Size: 43 8 519 252 1 286 286
Fragments arranged by size:
519 286 286 252 43 8 1
EcoRI G'AATT_C
Cuts at: 0 613 1395
Size: 613 782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 714 1395
Size: 714 681
FspI TGC'GCA
Cuts at: 0 541 1395
Size: 541 854
HaeII r_GCGC'y
Cuts at: 0 339 582 1047 1395
Size: 339 243 465 348
Fragments arranged by size:
465 348 339 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 372 404 457 489 746 778 862 894
Size: 372 32 53 32 257 32 84 32
Cuts at: 894 1033 1065 1395
Size: 139 32 330
Fragments arranged by size:
372 330 257 139 84 53 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 356 1240 1395
Size: 356 884 155
Fragments arranged by size:
884 356 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1395
Size: 824 571
MscI TGG'CCA
Cuts at: 0 668 1080 1395
Size: 668 412 315
Fragments arranged by size:
668 412 315
MslI CAynn'nnrTG
Cuts at: 0 889 1187 1395
Size: 889 298 208
Fragments arranged by size:
889 298 208
MspA1I CmG'CkG
Cuts at: 0 96 126 227 255 566 1105 1245 1374
Size: 96 30 101 28 311 539 140 129
Cuts at: 1374 1395
Size: 21
Fragments arranged by size:
539 311 140 129 101 96 30 28
21
NcoI C'CATG_G
Cuts at: 0 327 1395
Size: 327 1068
NspI r_CATG'y
Cuts at: 0 421 1395
Size: 421 974
PasI CC'CwG_GG
Cuts at: 0 33 291 477 819 1395
Size: 33 258 186 342 576
Fragments arranged by size:
576 342 258 186 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 1353 1395
Size: 39 1314 42
Fragments arranged by size:
1314 42 39
PfoI T'CCnGG_A
Cuts at: 0 178 980 1395
Size: 178 802 415
Fragments arranged by size:
802 415 178
PspOMI G'GGCC_C
Cuts at: 0 50 294 569 822 1108 1395
Size: 50 244 275 253 286 287
Fragments arranged by size:
287 286 275 253 244 50
PstI C_TGCA'G
Cuts at: 0 431 1189 1395
Size: 431 758 206
Fragments arranged by size:
758 431 206
PvuII CAG'CTG
Cuts at: 0 126 227 255 1105 1245 1374 1395
Size: 126 101 28 850 140 129 21
Fragments arranged by size:
850 140 129 126 101 28 21
SapI GCTCTTCn'nnn_
Cuts at: 0 1034 1395
Size: 1034 361
SfcI C'TryA_G
Cuts at: 0 427 1185 1395
Size: 427 758 210
Fragments arranged by size:
758 427 210
SmaI CCC'GGG
Cuts at: 0 517 1167 1395
Size: 517 650 228
Fragments arranged by size:
650 517 228
StuI AGG'CCT
Cuts at: 0 13 1395
Size: 13 1382
StyI C'CwwG_G
Cuts at: 0 46 327 677 1395
Size: 46 281 350 718
Fragments arranged by size:
718 350 281 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 129 223 461 603 698 832 1395
Size: 40 89 94 238 142 95 134 563
Fragments arranged by size:
563 238 142 134 95 94 89 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 847 879 1395
Size: 847 32 516
Fragments arranged by size:
847 516 32
Tth111I GACn'n_nGTC
Cuts at: 0 360 1395
Size: 360 1035
XmaI C'CCGG_G
Cuts at: 0 515 1165 1395
Size: 515 650 230
Fragments arranged by size:
650 515 230
Enzymes that do cut:
AccI AcuI AhdI AleI AlwNI ApaI ApoI AvaI
BanII BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NcoI NspI PasI PflMI PfoI PspOMI PstI
PvuII SapI SfcI SmaI StuI StyI TaqII TstI
Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I ClaI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoRV FseI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XcmI XhoI
XmnI ZraI