Restriction map for
pFN21AB8561
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# NO CUT ENZYMES ( 85 enzymes) #
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AccII AclI AcsI AflII AflIII
AocI ApoI AscI AseI AsnI
AviII BbeI BcgI BfrI BglII
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI EagI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KspI MfeI MluI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1819 GACGTC GACGT^C
Acc65I 84 GGTACC G^GTACC
AccI 1327 GTCTAC GT^[AC][GT]AC
AgeI 1953 ACCGGT A^CCGGT
AhdI 586 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1636 GTGCAC G^TGCAC
Aor51HI 491 AGCGCT AGC^GCT
ApaLI 1636 GTGCAC G^TGCAC
Asp718I 84 GGTACC G^GTACC
AspEI 586 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 832 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1096 CCTAGG C^CTAGG
BalI 2873 TGGCCA TGG^CCA
BamHI 854 GGATCC G^GATCC
BbrPI 364 CACGTG CAC^GTG
BbsI 412 GAAGAC GAAGAC(2/6)
BbvCI 273 GCTGAGG CCTCAGC(-5/-2)
BclI 1427 TGATCA T^GATCA
BlnI 1096 CCTAGG C^CTAGG
BlpI 225 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 225 GCTGAGC GC^T[ACGT]AGC
BpuAI 412 GAAGAC GAAGAC(2/6)
BsaBI 1467 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1756 CGTCTC CGTCTC(1/5)
BsmI 2085 GAATGC GAATGC(1/-1)
BssSI 1576 CTCGTG CACGAG(-5/-1)
BstAPI 1625 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 225 GCTGAGC GC^T[ACGT]AGC
DraIII 1453 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1645 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 586 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 491 AGCGCT AGC^GCT
FbaI 1427 TGATCA T^GATCA
HaeII 491 AGCGCT [GA]GCGC^[CT]
KpnI 84 GGTACC GGTAC^C
MamI 1467 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2873 TGGCCA TGG^CCA
MscI 2873 TGGCCA TGG^CCA
PflFI 832 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1953 ACCGGT A^CCGGT
PmaCI 364 CACGTG CAC^GTG
PmlI 364 CACGTG CAC^GTG
PshAI 1699 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 954 AGTACT AGT^ACT
SfiI 2874 GGCCAATCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 868 ACTAGT A^CTAGT
StuI 986 AGGCCT AGG^CCT
Tsp509I 1508 AATT ^AATT
Tth111I 832 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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ApaI 241 GGGCCC GGGCC^C
2434 GGGCCC
AvaI 2451 CCCGAG C^[CT]CG[GA]G
2581 CCCGAG
BciVI 116 GTATCC GTATCC(6/5)
1512 GTATCC
BsaAI 364 CACGTG [CT]AC^GT[GA]
2590 TACGTG
BsaI 787 GAGACC GGTCTC(1/5)
1922 GAGACC
BsoBI 2451 CCCGAG C^[CT]CG[GA]G
2581 CCCGAG
BsrBI 228 GAGCGG CCGCTC(-3/-3)
1543 GAGCGG
CfoI 492 GCGC GCG^C
2462 GCGC
EaeI 896 TGGCCG [CT]^GGCC[GA]
2873 TGGCCA
EarI 1480 GAAGAG CTCTTC(1/4)
2245 GAAGAG
HhaI 492 GCGC GCG^C
2462 GCGC
HinP1I 492 GCGC G^CGC
2462 GCGC
Ksp632I 1480 GAAGAG CTCTTC(1/4)
2245 GAAGAG
MseI 664 TTAA T^TAA
1790 TTAA
NspI 485 ACATGC [GA]CATG^[CT]
1841 GCATGT
SexAI 833 ACCTGGT A^CC[AT]GGT
2507 ACCTGGT
SmlI 121 CTCAAG C^T[CT][GA]AG
2554 CTCAAG
TfiI 1212 GATTC G^A[AT]TC
1281 GAATC
Tru9I 664 TTAA T^TAA
1790 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspHI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII DsaI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmnI