Restriction map for
pFN21AB8561
(Linear) (Six-base) MAPSORT of: KIBB8561.seq Check: 8120 from: 1 to: 2901
KIBB8561.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 898 2901
Size: 898 2003
AatII G_ACGT'C
Cuts at: 0 1823 2901
Size: 1823 1078
AccI GT'mk_AC
Cuts at: 0 1328 2901
Size: 1328 1573
Acc65I G'GTAC_C
Cuts at: 0 84 2901
Size: 84 2817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 301 451 943 1582 2901
Size: 77 224 150 492 639 1319
Fragments arranged by size:
1319 639 492 224 150 77
AfeI AGC'GCT
Cuts at: 0 493 2901
Size: 493 2408
AgeI A'CCGG_T
Cuts at: 0 1953 2901
Size: 1953 948
AhdI GACnn_n'nnGTC
Cuts at: 0 591 2901
Size: 591 2310
AleI CACnn'nnGTG
Cuts at: 0 1576 2901
Size: 1576 1325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 2901
Size: 541 2360
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1540 1572 2901
Size: 1540 32 1329
Fragments arranged by size:
1540 1329 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1021 2259 2706 2802 2901
Size: 1021 1238 447 96 99
Fragments arranged by size:
1238 1021 447 99 96
ApaI G_GGCC'C
Cuts at: 0 245 2438 2901
Size: 245 2193 463
Fragments arranged by size:
2193 463 245
ApaLI G'TGCA_C
Cuts at: 0 1636 2901
Size: 1636 1265
AvaI C'yCGr_G
Cuts at: 0 2451 2581 2901
Size: 2451 130 320
Fragments arranged by size:
2451 320 130
AvrII C'CTAG_G
Cuts at: 0 1096 2901
Size: 1096 1805
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 99 132 2901
Size: 99 33 2769
Fragments arranged by size:
2769 99 33
BamHI G'GATC_C
Cuts at: 0 854 2901
Size: 854 2047
BanI G'GyrC_C
Cuts at: 0 84 926 976 2695 2901
Size: 84 842 50 1719 206
Fragments arranged by size:
1719 842 206 84 50
BanII G_rGCy'C
Cuts at: 0 20 74 245 548 608 1490 2120 2438
Size: 20 54 171 303 60 882 630 318
Cuts at: 2438 2555 2567 2732 2901
Size: 117 12 165 169
Fragments arranged by size:
882 630 318 303 171 169 165 117
60 54 20 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 419 2901
Size: 419 2482
BbvCI CC'TCA_GC
Cuts at: 0 274 2901
Size: 274 2627
BciVI GTATCCnnnnn_n'
Cuts at: 0 127 1523 2901
Size: 127 1396 1378
Fragments arranged by size:
1396 1378 127
BclI T'GATC_A
Cuts at: 0 1427 2901
Size: 1427 1474
BglI GCCn_nnn'nGGC
Cuts at: 0 985 2454 2881 2901
Size: 985 1469 427 20
Fragments arranged by size:
1469 985 427 20
BlpI GC'TnA_GC
Cuts at: 0 226 2901
Size: 226 2675
Bme1580I G_kGCm'C
Cuts at: 0 43 245 981 1640 2438 2698 2901
Size: 43 202 736 659 798 260 203
Fragments arranged by size:
798 736 659 260 203 202 43
BmgBI CAC'GTC
Cuts at: 0 111 2901
Size: 111 2790
BmrI ACTGGGnnnn_n'
Cuts at: 0 1991 2901
Size: 1991 910
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2105 2901
Size: 2105 796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 958 1171 1561 1639 1906 1933 2443
Size: 562 396 213 390 78 267 27 510
Cuts at: 2443 2569 2901
Size: 126 332
Fragments arranged by size:
562 510 396 390 332 267 213 126
78 27
Bpu10I CC'TnA_GC
Cuts at: 0 22 274 547 721 1855 2338 2901
Size: 22 252 273 174 1134 483 563
Fragments arranged by size:
1134 563 483 273 252 174 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 2539 2901
Size: 106 2433 362
Fragments arranged by size:
2433 362 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 781 1916 2901
Size: 781 1135 985
Fragments arranged by size:
1135 985 781
BsaAI yAC'GTr
Cuts at: 0 366 2592 2901
Size: 366 2226 309
Fragments arranged by size:
2226 366 309
BsaBI GATnn'nnATC
Cuts at: 0 1471 2901
Size: 1471 1430
BsaHI Gr'CG_yC
Cuts at: 0 1820 2398 2831 2901
Size: 1820 578 433 70
Fragments arranged by size:
1820 578 433 70
BsaWI w'CCGG_w
Cuts at: 0 251 473 680 1655 1953 2495 2901
Size: 251 222 207 975 298 542 406
Fragments arranged by size:
975 542 406 298 251 222 207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 718 748 1540 1570 2338 2368 2901
Size: 718 30 792 30 768 30 533
Fragments arranged by size:
792 768 718 533 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 742 745 955 1498 2230 2362 2797 2901
Size: 742 3 210 543 732 132 435 104
Fragments arranged by size:
742 732 543 435 210 132 104 3
BseYI C'CCAG_C
Cuts at: 0 531 963 1527 1692 1982 2149 2233 2252
Size: 531 432 564 165 290 167 84 19
Cuts at: 2252 2457 2901
Size: 205 444
Fragments arranged by size:
564 531 444 432 290 205 167 165
84 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 346 361 1371 2049 2901
Size: 298 48 15 1010 678 852
Fragments arranged by size:
1010 852 678 298 48 15
BsiHKAI G_wGCw'C
Cuts at: 0 548 608 1073 1571 1640 2555 2567 2732
Size: 548 60 465 498 69 915 12 165
Cuts at: 2732 2901
Size: 169
Fragments arranged by size:
915 548 498 465 169 165 69 60
12
BsmI GAATG_Cn'
Cuts at: 0 2091 2901
Size: 2091 810
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1762 2901
Size: 1762 1139
Bsp1286I G_dGCh'C
Cuts at: 0 20 43 74 245 548 608 981 1073
Size: 20 23 31 171 303 60 373 92
Cuts at: 1073 1490 1571 1640 2120 2438 2555 2567 2698
Size: 417 81 69 480 318 117 12 131
Cuts at: 2698 2732 2901
Size: 34 169
Fragments arranged by size:
480 417 373 318 303 171 169 131
117 92 81 69 60 34 31 23
20 12
BspEI T'CCGG_A
Cuts at: 0 473 680 1655 2901
Size: 473 207 975 1246
Fragments arranged by size:
1246 975 473 207
BspHI T'CATG_A
Cuts at: 0 740 1412 1430 1970 2901
Size: 740 672 18 540 931
Fragments arranged by size:
931 740 672 540 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 898 1381 2849 2901
Size: 898 483 1468 52
Fragments arranged by size:
1468 898 483 52
BsrBI CCG'CTC
Cuts at: 0 230 1545 2901
Size: 230 1315 1356
Fragments arranged by size:
1356 1315 230
BsrDI GCAATG_nn'
Cuts at: 0 1256 1646 2063 2901
Size: 1256 390 417 838
Fragments arranged by size:
1256 838 417 390
BsrFI r'CCGG_y
Cuts at: 0 50 763 1055 1925 1953 2003 2901
Size: 50 713 292 870 28 50 898
Fragments arranged by size:
898 870 713 292 50 50 28
BssSI C'ACGA_G
Cuts at: 0 1576 2901
Size: 1576 1325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1631 2901
Size: 1631 1270
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 894 2112 2190 2871 2901
Size: 81 813 1218 78 681 30
Fragments arranged by size:
1218 813 681 81 78 30
BstYI r'GATC_y
Cuts at: 0 197 302 854 1133 1850 2901
Size: 197 105 552 279 717 1051
Fragments arranged by size:
1051 717 552 279 197 105
BtgI C'CryG_G
Cuts at: 0 74 1607 2069 2366 2901
Size: 74 1533 462 297 535
Fragments arranged by size:
1533 535 462 297 74
BtsI GCAGTG_nn'
Cuts at: 0 1430 1588 2901
Size: 1430 158 1313
Fragments arranged by size:
1430 1313 158
DraIII CAC_nnn'GTG
Cuts at: 0 1458 2901
Size: 1458 1443
DrdI GACnn_nn'nnGTC
Cuts at: 0 1651 2901
Size: 1651 1250
EaeI y'GGCC_r
Cuts at: 0 896 2873 2901
Size: 896 1977 28
Fragments arranged by size:
1977 896 28
EarI CTCTTCn'nnn_
Cuts at: 0 1475 2240 2901
Size: 1475 765 661
Fragments arranged by size:
1475 765 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 249 847 2901
Size: 249 598 2054
Fragments arranged by size:
2054 598 249
EcoICRI GAG'CTC
Cuts at: 0 546 606 2553 2565 2730 2901
Size: 546 60 1947 12 165 171
Fragments arranged by size:
1947 546 171 165 60 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 301 451 562 943 958 1171 1561
Size: 77 224 150 111 381 15 213 390
Cuts at: 1561 1582 1639 1906 1933 2443 2569 2901
Size: 21 57 267 27 510 126 332
Fragments arranged by size:
510 390 381 332 267 224 213 150
126 111 77 57 27 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 613 720 1015 2779 2901
Size: 433 180 107 295 1764 122
Fragments arranged by size:
1764 433 295 180 122 107
EcoO109I rG'GnC_Cy
Cuts at: 0 61 2434 2435 2901
Size: 61 2373 1 466
Fragments arranged by size:
2373 466 61 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 615 1890 2901
Size: 147 468 1275 1011
Fragments arranged by size:
1275 1011 468 147
HaeII r_GCGC'y
Cuts at: 0 495 2901
Size: 495 2406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 2338 2370 2901
Size: 577 32 1729 32 531
Fragments arranged by size:
1729 577 531 32 32
KpnI G_GTAC'C
Cuts at: 0 88 2901
Size: 88 2813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1821 2253 2901
Size: 620 1201 432 648
Fragments arranged by size:
1201 648 620 432
MscI TGG'CCA
Cuts at: 0 2875 2901
Size: 2875 26
MslI CAynn'nnrTG
Cuts at: 0 79 826 1576 1666 1906 2068 2901
Size: 79 747 750 90 240 162 833
Fragments arranged by size:
833 750 747 240 162 90 79
MspA1I CmG'CkG
Cuts at: 0 531 797 864 936 963 1979 2259 2574
Size: 531 266 67 72 27 1016 280 315
Cuts at: 2574 2901
Size: 327
Fragments arranged by size:
1016 531 327 315 280 266 72 67
27
NaeI GCC'GGC
Cuts at: 0 52 765 1057 2901
Size: 52 713 292 1844
Fragments arranged by size:
1844 713 292 52
NcoI C'CATG_G
Cuts at: 0 74 1607 2069 2366 2901
Size: 74 1533 462 297 535
Fragments arranged by size:
1533 535 462 297 74
NgoMIV G'CCGG_C
Cuts at: 0 50 763 1055 2901
Size: 50 713 292 1846
Fragments arranged by size:
1846 713 292 50
NspI r_CATG'y
Cuts at: 0 489 1845 2901
Size: 489 1356 1056
Fragments arranged by size:
1356 1056 489
PflMI CCAn_nnn'nTGG
Cuts at: 0 1252 1539 1769 2901
Size: 1252 287 230 1132
Fragments arranged by size:
1252 1132 287 230
PfoI T'CCnGG_A
Cuts at: 0 200 2129 2210 2294 2901
Size: 200 1929 81 84 607
Fragments arranged by size:
1929 607 200 84 81
PmlI CAC'GTG
Cuts at: 0 366 2901
Size: 366 2535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1540 1572 2901
Size: 1540 32 1329
Fragments arranged by size:
1540 1329 32
PshAI GACnn'nnGTC
Cuts at: 0 1703 2901
Size: 1703 1198
PspOMI G'GGCC_C
Cuts at: 0 241 2434 2901
Size: 241 2193 467
Fragments arranged by size:
2193 467 241
PstI C_TGCA'G
Cuts at: 0 69 530 1064 1186 1595 2207 2898 2901
Size: 69 461 534 122 409 612 691 3
Fragments arranged by size:
691 612 534 461 409 122 69 3
PvuII CAG'CTG
Cuts at: 0 531 864 963 2259 2574 2901
Size: 531 333 99 1296 315 327
Fragments arranged by size:
1296 531 333 327 315 99
SacI G_AGCT'C
Cuts at: 0 548 608 2555 2567 2732 2901
Size: 548 60 1947 12 165 169
Fragments arranged by size:
1947 548 169 165 60 12
ScaI AGT'ACT
Cuts at: 0 956 2901
Size: 956 1945
SexAI A'CCwGG_T
Cuts at: 0 833 2507 2901
Size: 833 1674 394
Fragments arranged by size:
1674 833 394
SfcI C'TryA_G
Cuts at: 0 65 135 526 1060 1182 1591 2203 2894
Size: 65 70 391 534 122 409 612 691
Cuts at: 2894 2901
Size: 7
Fragments arranged by size:
691 612 534 409 391 122 70 65
7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2881 2901
Size: 2881 20
SmlI C'TyrA_G
Cuts at: 0 121 2554 2901
Size: 121 2433 347
Fragments arranged by size:
2433 347 121
SpeI A'CTAG_T
Cuts at: 0 868 2901
Size: 868 2033
StuI AGG'CCT
Cuts at: 0 988 2901
Size: 988 1913
StyI C'CwwG_G
Cuts at: 0 74 1074 1096 1607 2069 2366 2864 2901
Size: 74 1000 22 511 462 297 498 37
Fragments arranged by size:
1000 511 498 462 297 74 37 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 332 2157 2713 2901
Size: 322 10 1825 556 188
Fragments arranged by size:
1825 556 322 188 10
TatI w'GTAC_w
Cuts at: 0 954 1583 2162 2901
Size: 954 629 579 739
Fragments arranged by size:
954 739 629 579
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 277 309 361 393 718 750 1092 1124
Size: 277 32 52 32 325 32 342 32
Cuts at: 1124 2317 2349 2456 2488 2901
Size: 1193 32 107 32 413
Fragments arranged by size:
1193 413 342 325 277 107 52 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 835 2901
Size: 835 2066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 1146 1614 2487 2901
Size: 252 894 468 873 414
Fragments arranged by size:
894 873 468 414 252
XmnI GAAnn'nnTTC
Cuts at: 0 163 748 806 2901
Size: 163 585 58 2095
Fragments arranged by size:
2095 585 163 58
ZraI GAC'GTC
Cuts at: 0 1821 2901
Size: 1821 1080
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AvaI AvrII
BaeI BamHI BanI BanII BbsI BbvCI BciVI BclI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI
PfoI PmlI PpiI PshAI PspOMI PstI PvuII SacI
ScaI SexAI SfcI SfiI SmlI SpeI StuI StyI
TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII ApoI AscI AseI AsiSI BbeI
BcgI BfrBI BglII BmtI BsiEI BsiWI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NarI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PpuMI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI SwaI XbaI XhoI XmaI