(Circular) (Six-base) MAPSORT of: pFN21AB6684.seq Check: 6359 from: 1 to: 6073 pFN21AB6684 6073 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2378 2388 2982 3416 1937 Size: 441 10 594 434 4594 Fragments arranged by size: 4594 594 441 434 10 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3377 4136 5520 5788 1632 Size: 1745 759 1384 268 1917 Fragments arranged by size: 1917 1745 1384 759 268 AfeI AGC'GCT Cuts at: 2615 2615 Size: 6073 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 2695 4972 5949 1203 Size: 1492 2277 977 1327 Fragments arranged by size: 2277 1492 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 3816 5388 3816 Size: 1572 4501 ApaI G_GGCC'C Cuts at: 2512 2512 Size: 6073 ApaLI G'TGCA_C Cuts at: 2795 4130 5286 2795 Size: 1335 1156 3582 Fragments arranged by size: 3582 1335 1156 ApoI r'AATT_y Cuts at: 1493 2821 3197 3382 3608 3760 5869 1493 Size: 1328 376 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 1328 376 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 4627 160 Size: 872 3595 1606 Fragments arranged by size: 3595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2426 3398 1870 Size: 73 483 972 4545 Fragments arranged by size: 4545 972 483 73 BamHI G'GATC_C Cuts at: 3403 3770 3403 Size: 367 5706 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 3394 4849 618 Size: 325 217 627 354 1253 1455 1842 Fragments arranged by size: 1842 1455 1253 627 354 325 217 BanII G_rGCy'C Cuts at: 1391 2512 3392 1391 Size: 1121 880 4072 Fragments arranged by size: 4072 1121 880 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3204 928 Size: 971 1305 3797 Fragments arranged by size: 3797 1305 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4264 4298 1903 Size: 34 50 34 2243 34 3678 Fragments arranged by size: 3678 2243 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 2779 1548 Size: 1231 4842 BfrBI ATG'CAT Cuts at: 3019 3019 Size: 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4684 136 Size: 107 122 71 631 605 3012 1525 Fragments arranged by size: 3012 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2430 6068 2430 Size: 3638 2435 BlpI GC'TnA_GC Cuts at: 2060 3490 2060 Size: 1430 4643 Bme1580I G_kGCm'C Cuts at: 1790 2512 2799 4134 5290 1790 Size: 722 287 1335 1156 2573 Fragments arranged by size: 2573 1335 1156 722 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3804 4762 442 Size: 416 53 83 399 24 2387 958 1753 Fragments arranged by size: 2387 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2651 2651 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2646 2837 4733 1239 Size: 1407 191 1896 2579 Fragments arranged by size: 2579 1896 1407 191 Bpu10I CC'TnA_GC Cuts at: 3089 3129 3089 Size: 40 6033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4198 5063 5361 4198 Size: 865 298 4910 Fragments arranged by size: 4910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4736 882 Size: 617 3237 2219 Fragments arranged by size: 3237 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3864 493 Size: 711 2660 2702 Fragments arranged by size: 2702 2660 711 BsaBI GATnn'nnATC Cuts at: 6067 6067 Size: 6073 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4262 4897 275 Size: 53 83 186 936 2347 382 635 1451 Fragments arranged by size: 2347 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3237 4506 4904 5178 5325 5736 3237 Size: 1269 398 274 147 411 3574 Fragments arranged by size: 3574 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5848 Fragments arranged by size: 5848 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 1186 Size: 970 5103 BseYI C'CCAG_C Cuts at: 2461 2551 5276 5772 2461 Size: 90 2725 496 2762 Fragments arranged by size: 2762 2725 496 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3247 1163 Size: 573 1511 3989 Fragments arranged by size: 3989 1511 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2799 3007 4134 4219 5290 2799 Size: 208 1127 85 1071 3582 Fragments arranged by size: 3582 1127 1071 208 85 BsmI GAATG_Cn' Cuts at: 2347 3584 3677 2347 Size: 1237 93 4743 Fragments arranged by size: 4743 1237 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2512 2799 3007 3392 4134 4219 5290 Size: 399 722 287 208 385 742 85 1071 Cuts at: 5290 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 722 399 385 287 208 85 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 3428 844 Size: 1903 681 3489 Fragments arranged by size: 3489 1903 681 BsrBI CCG'CTC Cuts at: 1394 1823 2962 3960 1394 Size: 429 1139 998 3507 Fragments arranged by size: 3507 1139 998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4568 4742 65 Size: 1183 3320 174 1396 Fragments arranged by size: 3320 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4717 4904 1700 Size: 110 2907 187 2869 Fragments arranged by size: 2907 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 2593 3029 4133 5145 2593 Size: 436 1104 1012 3521 Fragments arranged by size: 3521 1104 1012 436 BstAPI GCAn_nnn'nTGC Cuts at: 794 2342 794 Size: 1548 4525 BstBI TT'CG_AA Cuts at: 2180 4877 2180 Size: 2697 3376 BstEII G'GTnAC_C Cuts at: 1184 3079 1184 Size: 1895 4178 BstXI CCAn_nnnn'nTGG Cuts at: 2019 2231 2492 2019 Size: 212 261 5600 Fragments arranged by size: 5600 261 212 BstYI r'GATC_y Cuts at: 1972 2430 3403 3770 4155 4172 5624 5710 5722 Size: 458 973 367 385 17 1452 86 12 Cuts at: 5722 6068 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 973 458 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2269 2358 513 Size: 552 1204 89 4228 Fragments arranged by size: 4228 1204 552 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5334 Fragments arranged by size: 5334 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3264 3589 4383 4403 2221 Size: 1043 325 794 20 3891 Fragments arranged by size: 3891 1043 794 325 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2708 5080 809 Size: 507 1392 2372 1802 Fragments arranged by size: 2372 1802 1392 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3520 4409 6038 Size: 54 1425 50 275 18 1690 889 1629 Cuts at: 6038 8 Size: 43 Fragments arranged by size: 1690 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 2604 2642 3112 4003 1353 Size: 1190 61 38 470 891 3423 Fragments arranged by size: 3423 1190 891 470 61 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4660 5034 5180 387 Size: 1064 138 3071 374 146 1280 Fragments arranged by size: 3071 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2646 2837 3377 4136 4733 5520 5788 Size: 393 1014 191 540 759 597 787 268 Cuts at: 5788 1239 Size: 1524 Fragments arranged by size: 1524 1014 787 759 597 540 393 268 191 EcoNI CCTnn'n_nnAGG Cuts at: 2917 2917 Size: 6073 EcoO109I rG'GnC_Cy Cuts at: 2015 2044 2508 2015 Size: 29 464 5580 Fragments arranged by size: 5580 464 29 EcoRI G'AATT_C Cuts at: 2821 3382 3760 2821 Size: 561 378 5134 Fragments arranged by size: 5134 561 378 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 2127 2617 2814 5220 1678 Size: 449 490 197 2406 2531 Fragments arranged by size: 2531 2406 490 449 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2764 2796 4714 4746 4788 Size: 32 135 32 1192 32 1918 32 42 Cuts at: 4788 4820 1373 Size: 32 2626 Fragments arranged by size: 2626 1918 1192 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2713 3417 3663 669 Size: 988 281 775 704 246 3079 Fragments arranged by size: 3079 988 775 704 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2047 2451 2671 3240 5187 5371 1305 Size: 742 404 220 569 1947 184 2007 Fragments arranged by size: 2007 1947 742 569 404 220 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4251 Fragments arranged by size: 4251 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4031 4390 4549 518 Size: 648 2865 359 159 2042 Fragments arranged by size: 2865 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2399 2551 3489 4166 5314 5559 1606 Size: 793 152 938 677 1148 245 2120 Fragments arranged by size: 2120 1148 938 793 677 245 152 NcoI C'CATG_G Cuts at: 513 1065 2269 513 Size: 552 1204 4317 Fragments arranged by size: 4317 1204 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NsiI A_TGCA'T Cuts at: 3021 3021 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 2699 3019 3431 4976 1132 Size: 97 1470 320 412 1545 2229 Fragments arranged by size: 2229 1545 1470 412 320 97 PasI CC'CwG_GG Cuts at: 1348 2019 1348 Size: 671 5402 PciI A'CATG_T Cuts at: 2695 4972 2695 Size: 2277 3796 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6073 PfoI T'CCnGG_A Cuts at: 2300 2300 Size: 6073 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2015 2015 Size: 6073 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PspOMI G'GGCC_C Cuts at: 2508 2508 Size: 6073 PstI C_TGCA'G Cuts at: 830 3425 830 Size: 2595 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 2399 2551 2399 Size: 152 5921 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SapI GCTCTTCn'nnn_ Cuts at: 2543 2604 2543 Size: 61 6012 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SfcI C'TryA_G Cuts at: 826 1045 2379 3421 4556 5237 5428 826 Size: 219 1334 1042 1135 681 191 1471 Fragments arranged by size: 1471 1334 1135 1042 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 3400 1872 Size: 1528 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2426 4177 5078 5340 820 Size: 197 926 483 1751 901 262 1553 Fragments arranged by size: 1751 1553 926 901 483 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3019 3431 1132 Size: 1887 412 3774 Fragments arranged by size: 3774 1887 412 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 2568 2864 1517 Size: 1051 296 4726 Fragments arranged by size: 4726 1051 296 StyI C'CwwG_G Cuts at: 513 1065 2269 2918 2967 3512 513 Size: 552 1204 649 49 545 3074 Fragments arranged by size: 3074 1204 649 552 545 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2453 4100 4117 4270 4455 4900 Size: 1255 446 427 1647 17 153 185 445 Cuts at: 4900 325 Size: 1498 Fragments arranged by size: 1647 1498 1255 446 445 427 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2589 4319 6031 Size: 274 80 33 51 1443 612 1730 1712 Cuts at: 6031 96 Size: 138 Fragments arranged by size: 1730 1712 1443 612 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6033 6065 6033 Size: 32 6041 Tth111I GACn'n_nGTC Cuts at: 1316 2880 1316 Size: 1564 4509 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2729 2729 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 2426 1943 Size: 483 5590 XmaI C'CCGG_G Cuts at: 1870 3398 1870 Size: 1528 4545 XmnI GAAnn'nnTTC Cuts at: 4202 4202 Size: 6073 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI