(Circular) (Six-base) MAPSORT of: pFN21AB6680.seq Check: 9829 from: 1 to: 6073 pFN21AB6680 6073 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3416 1937 Size: 1479 4594 Acc65I G'GTAC_C Cuts at: 2424 3394 2424 Size: 970 5103 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2560 2744 2881 3267 4136 5520 5788 Size: 516 412 184 137 386 869 1384 268 Cuts at: 5788 1632 Size: 1917 Fragments arranged by size: 1917 1384 869 516 412 386 268 184 137 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 2998 3816 5388 2998 Size: 818 1572 3683 Fragments arranged by size: 3683 1572 818 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 3382 3608 3760 5869 1493 Size: 1889 226 152 2109 1697 Fragments arranged by size: 2109 1889 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4627 160 Size: 872 3595 1606 Fragments arranged by size: 3595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2529 3233 3398 1870 Size: 73 115 471 704 165 4545 Fragments arranged by size: 4545 704 471 165 115 73 BamHI G'GATC_C Cuts at: 3403 3770 3403 Size: 367 5706 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2424 3394 4849 618 Size: 325 217 627 637 970 1455 1842 Fragments arranged by size: 1842 1455 970 637 627 325 217 BanII G_rGCy'C Cuts at: 1391 2493 3105 3138 3392 1391 Size: 1102 612 33 254 4072 Fragments arranged by size: 4072 1102 612 254 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3214 3248 4264 4298 1903 Size: 34 1277 34 1016 34 3678 Fragments arranged by size: 3678 1277 1016 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 1548 Size: 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2561 3254 4684 Size: 107 122 71 631 605 889 693 1430 Cuts at: 4684 136 Size: 1525 Fragments arranged by size: 1525 1430 889 693 631 605 122 107 71 BglII A'GATC_T Cuts at: 6068 6068 Size: 6073 BlpI GC'TnA_GC Cuts at: 3023 3490 3023 Size: 467 5606 Bme1580I G_kGCm'C Cuts at: 1790 2380 4134 5290 1790 Size: 590 1754 1156 2573 Fragments arranged by size: 2573 1754 1156 590 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3804 4762 442 Size: 416 53 83 399 24 2387 958 1753 Fragments arranged by size: 2387 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2717 1056 Size: 1661 4412 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2947 2947 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 2423 2561 2702 3020 3062 3137 3221 Size: 1163 21 138 141 318 42 75 84 Cuts at: 3221 4733 1239 Size: 1512 2579 Fragments arranged by size: 2579 1512 1163 318 141 138 84 75 42 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2567 4198 5063 5361 2567 Size: 1631 865 298 3279 Fragments arranged by size: 3279 1631 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2306 4736 882 Size: 617 807 2430 2219 Fragments arranged by size: 2430 2219 807 617 BsaAI yAC'GTr Cuts at: 493 1204 2375 3864 493 Size: 711 1171 1489 2702 Fragments arranged by size: 2702 1489 1171 711 BsaBI GATnn'nnATC Cuts at: 2690 6067 2690 Size: 3377 2696 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4262 4897 275 Size: 53 83 186 936 2347 382 635 1451 Fragments arranged by size: 2347 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4506 4904 5178 5325 5736 4506 Size: 398 274 147 411 4843 Fragments arranged by size: 4843 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2241 2271 1178 Size: 30 165 30 838 30 4980 Fragments arranged by size: 4980 838 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2190 2294 2399 2723 2756 2924 1186 Size: 1004 104 105 324 33 168 4335 Fragments arranged by size: 4335 1004 324 168 105 104 33 BseYI C'CCAG_C Cuts at: 2078 2440 3018 3035 3123 5276 5772 2078 Size: 362 578 17 88 2153 496 2379 Fragments arranged by size: 2379 2153 578 496 362 88 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5500 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 3138 4134 4219 5290 2493 Size: 645 996 85 1071 3276 Fragments arranged by size: 3276 1071 996 645 85 BsmI GAATG_Cn' Cuts at: 2459 3584 3677 2459 Size: 1125 93 4855 Fragments arranged by size: 4855 1125 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2974 2974 Size: 6073 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2380 2493 3105 3138 3392 4134 4219 Size: 399 590 113 612 33 254 742 85 Cuts at: 4219 5290 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 612 590 399 254 113 85 33 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3428 844 Size: 2584 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3960 1394 Size: 429 2137 3507 Fragments arranged by size: 3507 2137 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2320 4568 4742 65 Size: 1183 1072 2248 174 1396 Fragments arranged by size: 2248 1396 1183 1072 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2771 4717 4904 1700 Size: 110 961 1946 187 2869 Fragments arranged by size: 2869 1946 961 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 4877 4877 Size: 6073 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstYI r'GATC_y Cuts at: 1972 2412 3093 3403 3770 4155 4172 5624 5710 Size: 440 681 310 367 385 17 1452 86 Cuts at: 5710 5722 6068 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 681 440 385 367 346 310 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2856 513 Size: 552 1791 3730 Fragments arranged by size: 3730 1791 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2307 487 Size: 683 56 1081 4253 Fragments arranged by size: 4253 1081 683 56 BtsI GCAGTG_nn' Cuts at: 3589 4383 4403 3589 Size: 794 20 5259 Fragments arranged by size: 5259 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DraIII CAC_nnn'GTG Cuts at: 2807 2807 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2508 2667 2769 Size: 54 1425 50 275 18 678 159 102 Cuts at: 2769 2773 3054 3520 4409 6038 8 Size: 4 281 466 889 1629 43 Fragments arranged by size: 1629 1425 889 678 466 281 275 159 102 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2167 2481 2733 3286 3321 4003 1353 Size: 814 314 252 553 35 682 3423 Fragments arranged by size: 3423 814 682 553 314 252 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2876 4660 5034 5180 387 Size: 1064 138 1287 1784 374 146 1280 Fragments arranged by size: 1784 1287 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2491 3136 2491 Size: 645 5428 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2402 2423 2560 2561 2702 2744 Size: 393 516 254 21 137 1 141 42 Cuts at: 2744 2881 3020 3062 3137 3221 3267 4136 4733 Size: 137 139 42 75 84 46 869 597 Cuts at: 4733 5520 5788 1239 Size: 787 268 1524 Fragments arranged by size: 1524 869 787 597 516 393 268 254 141 139 137 137 84 75 46 42 42 21 1 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2384 2609 2705 3233 2366 Size: 18 225 96 528 5206 Fragments arranged by size: 5206 528 225 96 18 EcoO109I rG'GnC_Cy Cuts at: 2698 2698 Size: 6073 EcoRI G'AATT_C Cuts at: 3382 3760 3382 Size: 378 5695 FseI GG_CCGG'CC Cuts at: 2775 2775 Size: 6073 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 5220 1678 Size: 3542 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2999 3031 3139 3171 4714 Size: 32 135 32 1427 32 108 32 1543 Cuts at: 4714 4746 4788 4820 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1543 1427 135 108 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3417 3663 669 Size: 988 281 1479 246 3079 Fragments arranged by size: 3079 1479 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 2428 3398 2428 Size: 970 5103 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2958 5187 5371 1305 Size: 1653 2229 184 2007 Fragments arranged by size: 2229 2007 1653 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 2669 10 Size: 54 1425 343 678 159 3414 Fragments arranged by size: 3414 1425 678 343 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2942 3318 4031 4390 4549 518 Size: 648 1776 376 713 359 159 2042 Fragments arranged by size: 2042 1776 713 648 376 359 159 MspA1I CmG'CkG Cuts at: 1606 2767 3085 3127 3489 4166 5314 5559 1606 Size: 1161 318 42 362 677 1148 245 2120 Fragments arranged by size: 2120 1161 1148 677 362 318 245 42 NaeI GCC'GGC Cuts at: 2773 2773 Size: 6073 NcoI C'CATG_G Cuts at: 513 1065 2856 513 Size: 552 1791 3730 Fragments arranged by size: 3730 1791 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NgoMIV G'CCGG_C Cuts at: 2771 2771 Size: 6073 NheI G'CTAG_C Cuts at: 1052 2713 1052 Size: 1661 4412 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 3431 4976 1132 Size: 97 2202 1545 2229 Fragments arranged by size: 2229 2202 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6073 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6073 PfoI T'CCnGG_A Cuts at: 2493 2538 2649 2493 Size: 45 111 5917 Fragments arranged by size: 5917 111 45 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2876 2908 4142 4174 5655 5687 2876 Size: 32 1234 32 1481 32 3262 Fragments arranged by size: 3262 1481 1234 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PspXI vC'TCGA_Gb Cuts at: 3233 3233 Size: 6073 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3152 3184 3152 Size: 32 6041 PstI C_TGCA'G Cuts at: 830 3015 3090 3425 830 Size: 2185 75 335 3478 Fragments arranged by size: 3478 2185 335 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 2767 3085 3127 2767 Size: 318 42 5713 Fragments arranged by size: 5713 318 42 SacI G_AGCT'C Cuts at: 2493 3138 2493 Size: 645 5428 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SapI GCTCTTCn'nnn_ Cuts at: 2481 2481 Size: 6073 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SfcI C'TryA_G Cuts at: 826 1045 2461 3011 3086 3421 4556 5237 5428 Size: 219 1416 550 75 335 1135 681 191 Cuts at: 5428 826 Size: 1471 Fragments arranged by size: 1471 1416 1135 681 550 335 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 2531 3400 1872 Size: 659 869 4545 Fragments arranged by size: 4545 869 659 SmlI C'TyrA_G Cuts at: 820 1017 1943 2582 3233 4177 5078 5340 820 Size: 197 926 639 651 944 901 262 1553 Fragments arranged by size: 1553 944 926 901 651 639 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 2888 1517 Size: 1371 4702 StyI C'CwwG_G Cuts at: 513 1065 2856 3512 513 Size: 552 1791 656 3074 Fragments arranged by size: 3074 1791 656 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4100 4117 4270 4455 4900 325 Size: 1255 2520 17 153 185 445 1498 Fragments arranged by size: 2520 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3168 4319 6031 Size: 274 80 33 51 1443 1191 1151 1712 Cuts at: 6031 96 Size: 138 Fragments arranged by size: 1712 1443 1191 1151 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6033 6065 6033 Size: 32 6041 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 2677 3104 2398 Size: 279 427 5367 Fragments arranged by size: 5367 427 279 XhoI C'TCGA_G Cuts at: 1943 3233 1943 Size: 1290 4783 XmaI C'CCGG_G Cuts at: 1870 2529 3398 1870 Size: 659 869 4545 Fragments arranged by size: 4545 869 659 XmnI GAAnn'nnTTC Cuts at: 2753 4202 2753 Size: 1449 4624 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI