Restriction map for
pFN21AB6674
(Circular) (Six-base) MAPSORT of: pFN21AB6674.seq Check: 8883 from: 1 to: 6067
pFN21AB6674 6067 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2790 2790
Size: 6067
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4130 5514 5782 1632
Size: 2498 1384 268 1917
Fragments arranged by size:
2498 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2089 2608 3810 5382 2089
Size: 519 1202 1572 2774
Fragments arranged by size:
2774 1572 1202 519
ApaI G_GGCC'C
Cuts at: 3206 3276 3206
Size: 70 5997
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 3071 3376 3602 3754 5863 1493
Size: 1578 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 1578 305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2305 2561 3392 1870
Size: 73 362 256 831 4545
Fragments arranged by size:
4545 831 362 256 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 4843 618
Size: 325 217 627 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2312 2601 3206 3276 3386 1391
Size: 921 289 605 70 110 4072
Fragments arranged by size:
4072 921 605 289 110 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2002 928
Size: 971 103 4993
Fragments arranged by size:
4993 971 103
BbvCI CC'TCA_GC
Cuts at: 2991 2991
Size: 6067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2714 2748 4258 4292 1903
Size: 34 777 34 1510 34 3678
Fragments arranged by size:
3678 1510 777 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3025 3959 5169 3025
Size: 934 1210 3923
Fragments arranged by size:
3923 1210 934
BclI T'GATC_A
Cuts at: 1548 2415 1548
Size: 867 5200
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2256 3280 4678
Size: 107 122 71 631 605 584 1024 1398
Cuts at: 4678 136
Size: 1525
Fragments arranged by size:
1525 1398 1024 631 605 584 122 107
71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 2216 3484 2216
Size: 1268 4799
Bme1580I G_kGCm'C
Cuts at: 1790 3206 3276 4128 5284 1790
Size: 1416 70 852 1156 2573
Fragments arranged by size:
2573 1416 1156 852 70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 2758 2872
Size: 416 53 83 399 24 1169 172 114
Cuts at: 2872 3200 3208 3798 4756 442
Size: 328 8 590 958 1753
Fragments arranged by size:
1753 1169 958 590 416 399 328 172
114 83 53 24 8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3118 3118
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2428 2507 2585 3153 4727 1239
Size: 911 278 79 78 568 1574 2579
Fragments arranged by size:
2579 1574 911 568 278 79 78
Bpu10I CC'TnA_GC
Cuts at: 2991 2991
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 4192 5057 5355 2387
Size: 1805 865 298 3099
Fragments arranged by size:
3099 1805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4730 882
Size: 617 3231 2219
Fragments arranged by size:
3231 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2805 3874 4256 4891
Size: 53 83 186 936 1272 1069 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1451 1272 1069 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 1178
Size: 30 165 30 1077 30 4735
Fragments arranged by size:
4735 1077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2952 1186
Size: 856 910 4301
Fragments arranged by size:
4301 910 856
BseYI C'CCAG_C
Cuts at: 2193 2243 2254 5270 5766 2193
Size: 50 11 3016 496 2494
Fragments arranged by size:
3016 2494 496 50 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2249 1163
Size: 573 513 4981
Fragments arranged by size:
4981 573 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2601 2925 4128 4213 5284 2160
Size: 441 324 1203 85 1071 2943
Fragments arranged by size:
2943 1203 1071 441 324 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2312 2601 2925 3206 3276 3386
Size: 399 370 152 289 324 281 70 110
Cuts at: 3386 4128 4213 5284 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 324 289 281
152 110 85 70
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 2790 3422 844
Size: 1563 383 632 3489
Fragments arranged by size:
3489 1563 632 383
BsrBI CCG'CTC
Cuts at: 1394 1823 2491 3954 1394
Size: 429 668 1463 3507
Fragments arranged by size:
3507 1463 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2142 96
Size: 2046 4021
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 2346 4127 5139 2346
Size: 1781 1012 3274
Fragments arranged by size:
3274 1781 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 2437 3397 3764 4149 4166 5618 5704 5716
Size: 465 960 367 385 17 1452 86 12
Cuts at: 5716 6062 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 960 465 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2849 3187 513
Size: 552 939 845 338 3393
Fragments arranged by size:
3393 939 845 552 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2567 487
Size: 683 56 1341 3987
Fragments arranged by size:
3987 1341 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 2674 3583 4377 4397 2177
Size: 497 909 794 20 3847
Fragments arranged by size:
3847 909 794 497 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DraIII CAC_nnn'GTG
Cuts at: 2326 2326
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2314 2664 3514
Size: 54 1425 50 275 18 484 350 850
Cuts at: 3514 4403 6032 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 850 484 350 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 3017 3997 1353
Size: 1023 641 980 3423
Fragments arranged by size:
3423 1023 980 641
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2282 4654 5028 5174 387
Size: 1064 138 693 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 693 374 146 138
EcoICRI GAG'CTC
Cuts at: 2599 2599
Size: 6067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2428 2507 2585 3153 4130 4727
Size: 393 518 278 79 78 568 977 597
Cuts at: 4727 5514 5782 1239
Size: 787 268 1524
Fragments arranged by size:
1524 977 787 597 568 518 393 278
268 79 78
EcoNI CCTnn'n_nnAGG
Cuts at: 3026 3026
Size: 6067
EcoO109I rG'GnC_Cy
Cuts at: 2149 3202 3272 3273 2149
Size: 1053 70 1 4943
Fragments arranged by size:
4943 1053 70 1
EcoRI G'AATT_C
Cuts at: 3071 3376 3754 3071
Size: 305 378 5384
Fragments arranged by size:
5384 378 305
FspI TGC'GCA
Cuts at: 2504 4573 2504
Size: 2069 3998
FspAI rTGC'GCAy
Cuts at: 2504 2504
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 5214 1678
Size: 3536 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2266 2298 2863 2895 4708
Size: 32 135 32 694 32 565 32 1813
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1813 694 565 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5181 5365 1305
Size: 3876 184 2007
Fragments arranged by size:
3876 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2316 10
Size: 54 1425 343 484 3761
Fragments arranged by size:
3761 1425 484 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 2736 4025 4384 4543 518
Size: 648 1386 184 1289 359 159 2042
Fragments arranged by size:
2042 1386 1289 648 359 184 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2089 2189 2197 2236 2254 3286 3483
Size: 438 45 100 8 39 18 1032 197
Cuts at: 3483 4160 5308 5553 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1032 677 438 245 197 100
45 39 18 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 3187 513
Size: 552 939 1183 3393
Fragments arranged by size:
3393 1183 939 552
NdeI CA'TA_TG
Cuts at: 387 2392 387
Size: 2005 4062
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 2717 2762 3425 4970 1132
Size: 97 1488 45 663 1545 2229
Fragments arranged by size:
2229 1545 1488 663 97 45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2608 3173 1108
Size: 1131 369 565 4002
Fragments arranged by size:
4002 1131 565 369
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2149
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 3202 3272 3202
Size: 70 5997
PstI C_TGCA'G
Cuts at: 830 3281 3419 830
Size: 2451 138 3478
Fragments arranged by size:
3478 2451 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2089 2189 2197 2236 2254 2089
Size: 100 8 39 18 5902
Fragments arranged by size:
5902 100 39 18 8
SacI G_AGCT'C
Cuts at: 2601 2601
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SapI GCTCTTCn'nnn_
Cuts at: 2376 2376
Size: 6067
SbfI CC_TGCA'GG
Cuts at: 3281 3419 3281
Size: 138 5929
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SexAI A'CCwGG_T
Cuts at: 2151 2409 2151
Size: 258 5809
SfcI C'TryA_G
Cuts at: 826 1045 2650 3277 3415 4550 5231 5422 826
Size: 219 1605 627 138 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 681 627 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 4171 5072 5334 820
Size: 197 926 423 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 423 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 2717 2762 3425 1132
Size: 1585 45 663 3774
Fragments arranged by size:
3774 1585 663 45
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 2072 1517
Size: 555 5512
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 3187 3506 513
Size: 552 939 201 982 319 3074
Fragments arranged by size:
3074 982 939 552 319 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 4111 4264 4449 4894 325
Size: 1255 2514 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 2719 4313
Size: 274 80 33 51 1443 165 577 1594
Cuts at: 4313 6025 96
Size: 1712 138
Fragments arranged by size:
1712 1594 1443 577 274 165 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3153 3185 6027 6059 3153
Size: 32 2842 32 3161
Fragments arranged by size:
3161 2842 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2057 1316
Size: 741 5326
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2240 3122 2240
Size: 882 5185
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI