(Circular) (Six-base) MAPSORT of: pFN21AB6640.seq Check: 9671 from: 1 to: 6031 pFN21AB6640 6031 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3374 1937 Size: 1437 4594 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2451 2583 4094 5478 5746 1632 Size: 819 132 1511 1384 268 1917 Fragments arranged by size: 1917 1511 1384 819 268 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 2287 4930 5907 1203 Size: 1084 2643 977 1327 Fragments arranged by size: 2643 1327 1084 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6031 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2145 1184 Size: 961 5070 AlwNI CAG_nnn'CTG Cuts at: 3774 5346 3774 Size: 1572 4459 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 2715 2724 2904 3169 3340 3566 3718 5827 Size: 1222 9 180 265 171 226 152 2109 Cuts at: 5827 1493 Size: 1697 Fragments arranged by size: 2109 1697 1222 265 226 180 171 152 9 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 3356 1870 Size: 73 1413 4545 Fragments arranged by size: 4545 1413 73 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2858 3352 4807 618 Size: 325 217 627 1071 494 1455 1842 Fragments arranged by size: 1842 1455 1071 627 494 325 217 BanII G_rGCy'C Cuts at: 1391 2317 3350 1391 Size: 926 1033 4072 Fragments arranged by size: 4072 1033 926 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2439 2520 928 Size: 971 540 81 4439 Fragments arranged by size: 4439 971 540 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4222 4256 1903 Size: 34 2285 34 3678 Fragments arranged by size: 3678 2285 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4642 136 Size: 107 122 71 631 605 2970 1525 Fragments arranged by size: 2970 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 2316 3448 2316 Size: 1132 4899 Bme1580I G_kGCm'C Cuts at: 1790 3280 4092 5248 1790 Size: 1490 812 1156 2573 Fragments arranged by size: 2573 1490 1156 812 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2051 3762 4720 Size: 416 53 83 399 24 634 1711 958 Cuts at: 4720 442 Size: 1753 Fragments arranged by size: 1753 1711 958 634 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2552 1056 Size: 1496 4535 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4691 1239 Size: 3452 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4156 5021 5319 4156 Size: 865 298 4868 Fragments arranged by size: 4868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2039 4694 882 Size: 617 540 2655 2219 Fragments arranged by size: 2655 2219 617 540 BsaAI yAC'GTr Cuts at: 493 1204 3275 3822 493 Size: 711 2071 547 2702 Fragments arranged by size: 2702 2071 711 547 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3838 4220 4855 275 Size: 53 83 186 936 2305 382 635 1451 Fragments arranged by size: 2305 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5806 Fragments arranged by size: 5806 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3312 1186 Size: 2126 3905 BseYI C'CCAG_C Cuts at: 5234 5730 5234 Size: 496 5535 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5458 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3481 4242 5270 1540 Size: 275 186 1480 761 1028 2301 Fragments arranged by size: 2301 1480 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4092 4177 5248 4092 Size: 85 1071 4875 Fragments arranged by size: 4875 1071 85 BsmI GAATG_Cn' Cuts at: 2693 3542 3635 2693 Size: 849 93 5089 Fragments arranged by size: 5089 849 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2317 3280 3350 4092 4177 5248 1391 Size: 399 527 963 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 963 742 527 399 85 70 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3092 3386 844 Size: 2248 294 3489 Fragments arranged by size: 3489 2248 294 BsrBI CCG'CTC Cuts at: 1394 1823 3918 1394 Size: 429 2095 3507 Fragments arranged by size: 3507 2095 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4526 4700 65 Size: 1183 3278 174 1396 Fragments arranged by size: 3278 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4675 4862 1700 Size: 110 2865 187 2869 Fragments arranged by size: 2869 2865 187 110 BsrGI T'GTAC_A Cuts at: 96 3019 96 Size: 2923 3108 BssHII G'CGCG_C Cuts at: 1925 2026 1925 Size: 101 5930 BssSI C'ACGA_G Cuts at: 4091 5103 4091 Size: 1012 5019 BstAPI GCAn_nnn'nTGC Cuts at: 794 2270 3160 794 Size: 1476 890 3665 Fragments arranged by size: 3665 1476 890 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 3361 3728 4113 4130 5582 5668 5680 6026 Size: 1389 367 385 17 1452 86 12 346 Cuts at: 6026 1972 Size: 1977 Fragments arranged by size: 1977 1452 1389 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 513 Size: 552 951 4528 Fragments arranged by size: 4528 951 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 2529 2571 3547 4341 4361 2529 Size: 42 976 794 20 4199 Fragments arranged by size: 4199 976 794 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2275 3478 4367 Size: 54 1425 50 275 18 445 1203 889 Cuts at: 4367 5996 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1203 889 445 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3478 1537 Size: 275 1666 4090 Fragments arranged by size: 4090 1666 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2599 2808 3961 1353 Size: 1246 209 1153 3423 Fragments arranged by size: 3423 1246 1153 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4618 4992 5138 387 Size: 1064 138 3029 374 146 1280 Fragments arranged by size: 3029 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2583 4094 4691 5478 5746 1239 Size: 393 819 132 1511 597 787 268 1524 Fragments arranged by size: 1524 1511 819 787 597 393 268 132 EcoNI CCTnn'n_nnAGG Cuts at: 2870 2870 Size: 6031 EcoO109I rG'GnC_Cy Cuts at: 3259 3259 Size: 6031 EcoRI G'AATT_C Cuts at: 2904 3340 3718 2904 Size: 436 378 5217 Fragments arranged by size: 5217 436 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2806 2806 Size: 6031 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 5178 1678 Size: 3500 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2510 2542 4672 4704 4746 Size: 32 135 32 938 32 2130 32 42 Cuts at: 4746 4778 1373 Size: 32 2626 Fragments arranged by size: 2626 2130 938 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3241 3375 3621 669 Size: 988 281 1303 134 246 3079 Fragments arranged by size: 3079 1303 988 281 246 134 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 2462 3195 3630 2462 Size: 733 435 4863 Fragments arranged by size: 4863 733 435 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5145 5329 1305 Size: 3840 184 2007 Fragments arranged by size: 3840 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2277 10 Size: 54 1425 343 445 3764 Fragments arranged by size: 3764 1425 445 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2665 3989 4348 4507 518 Size: 648 1499 1324 359 159 2042 Fragments arranged by size: 2042 1499 1324 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 3447 4124 5272 5517 1606 Size: 412 1429 677 1148 245 2120 Fragments arranged by size: 2120 1429 1148 677 412 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5479 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NheI G'CTAG_C Cuts at: 1052 2548 1052 Size: 1496 4535 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 2291 3389 4934 1132 Size: 97 1062 1098 1545 2229 Fragments arranged by size: 2229 1545 1098 1062 97 PasI CC'CwG_GG Cuts at: 1348 2708 1348 Size: 1360 4671 PciI A'CATG_T Cuts at: 2287 4930 2287 Size: 2643 3388 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6031 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3259 3259 Size: 6031 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 2490 3601 2490 Size: 1111 4920 PstI C_TGCA'G Cuts at: 830 2266 3383 830 Size: 1436 1117 3478 Fragments arranged by size: 3478 1436 1117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 SacII CC_GC'GG Cuts at: 2019 2019 Size: 6031 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 2262 3379 4514 5195 5386 826 Size: 219 1217 1117 1135 681 191 1471 Fragments arranged by size: 1471 1217 1135 1117 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4135 5036 5298 820 Size: 197 926 2192 901 262 1553 Fragments arranged by size: 2192 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 3007 152 Size: 2855 3176 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 3470 513 Size: 552 2405 3074 Fragments arranged by size: 3074 2405 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2053 4058 4075 4228 4413 4858 325 Size: 1255 473 2005 17 153 185 445 1498 Fragments arranged by size: 2005 1498 1255 473 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2390 2817 3019 Size: 274 80 33 51 1443 413 427 202 Cuts at: 3019 4277 5989 96 Size: 1258 1712 138 Fragments arranged by size: 1712 1443 1258 427 413 274 202 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5991 6023 5991 Size: 32 5999 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3292 3367 3292 Size: 75 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 2789 3063 4160 2789 Size: 274 1097 4660 Fragments arranged by size: 4660 1097 274 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI