(Circular) (Six-base) MAPSORT of: pFN21AB6632.seq Check: 2001 from: 1 to: 6025 pFN21AB6632 6025 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2326 2380 3159 2326 Size: 54 779 5192 Fragments arranged by size: 5192 779 54 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3368 1937 Size: 1431 4594 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2867 4088 5472 5740 1632 Size: 557 678 1221 1384 268 1917 Fragments arranged by size: 1917 1384 1221 678 557 268 AfeI AGC'GCT Cuts at: 2023 2023 Size: 6025 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 2268 2931 4924 5901 1203 Size: 1065 663 1993 977 1327 Fragments arranged by size: 1993 1327 1065 977 663 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AlwNI CAG_nnn'CTG Cuts at: 2849 3768 5340 2849 Size: 919 1572 3534 Fragments arranged by size: 3534 1572 919 ApaLI G'TGCA_C Cuts at: 4082 5238 4082 Size: 1156 4869 ApoI r'AATT_y Cuts at: 1493 2940 3334 3560 3712 5821 1493 Size: 1447 394 226 152 2109 1697 Fragments arranged by size: 2109 1697 1447 394 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2717 3350 1870 Size: 73 197 577 633 4545 Fragments arranged by size: 4545 633 577 197 73 BamHI G'GATC_C Cuts at: 2607 2803 3355 3722 2607 Size: 196 552 367 4910 Fragments arranged by size: 4910 552 367 196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2257 2294 2648 2678 Size: 325 217 627 255 215 37 354 30 Cuts at: 2678 2782 3128 3346 4801 618 Size: 104 346 218 1455 1842 Fragments arranged by size: 1842 1455 627 354 346 325 255 218 217 215 104 37 30 BanII G_rGCy'C Cuts at: 1391 2109 3344 1391 Size: 718 1235 4072 Fragments arranged by size: 4072 1235 718 BbeI G_GCGC'C Cuts at: 2046 2046 Size: 6025 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2234 928 Size: 971 335 4719 Fragments arranged by size: 4719 971 335 BbvCI CC'TCA_GC Cuts at: 3156 3237 3285 3156 Size: 81 48 5896 Fragments arranged by size: 5896 81 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 4636 136 Size: 107 122 71 631 605 421 2543 1525 Fragments arranged by size: 2543 1525 631 605 421 122 107 71 BglII A'GATC_T Cuts at: 3309 6020 3309 Size: 2711 3314 BlpI GC'TnA_GC Cuts at: 2025 3442 2025 Size: 1417 4608 Bme1580I G_kGCm'C Cuts at: 1790 2139 2262 2683 3133 4086 5242 1790 Size: 349 123 421 450 953 1156 2573 Fragments arranged by size: 2573 1156 953 450 421 349 123 BmgBI CAC'GTC Cuts at: 1318 2932 1318 Size: 1614 4411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2300 3282 3756 Size: 416 53 83 399 24 883 982 474 Cuts at: 3756 4714 442 Size: 958 1753 Fragments arranged by size: 1753 982 958 883 474 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2231 2417 2756 2826 2880 3031 4685 Size: 884 108 186 339 70 54 151 1654 Cuts at: 4685 1239 Size: 2579 Fragments arranged by size: 2579 1654 884 339 186 151 108 70 54 Bpu10I CC'TnA_GC Cuts at: 3156 3231 3237 3285 3156 Size: 75 6 48 5896 Fragments arranged by size: 5896 75 48 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3112 4688 882 Size: 617 1613 1576 2219 Fragments arranged by size: 2219 1613 1576 617 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 2043 3832 4214 Size: 53 83 186 936 483 27 1789 382 Cuts at: 4214 4849 275 Size: 635 1451 Fragments arranged by size: 1789 1451 936 635 483 382 186 83 53 27 BsaWI w'CCGG_w Cuts at: 2604 4458 4856 5130 5277 5688 2604 Size: 1854 398 274 147 411 2941 Fragments arranged by size: 2941 1854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2524 2554 3029 3059 1178 Size: 30 165 30 1121 30 475 30 4144 Fragments arranged by size: 4144 1121 475 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2782 2868 3254 1186 Size: 1596 86 386 3957 Fragments arranged by size: 3957 1596 386 86 BseYI C'CCAG_C Cuts at: 2083 2088 2261 2624 2828 2978 5228 5724 2083 Size: 5 173 363 204 150 2250 496 2384 Fragments arranged by size: 2384 2250 496 363 204 173 150 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2090 2921 1163 Size: 573 354 831 4267 Fragments arranged by size: 4267 831 573 354 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2842 4086 4171 5242 2842 Size: 1244 85 1071 3625 Fragments arranged by size: 3625 1244 1071 85 BsmI GAATG_Cn' Cuts at: 3536 3629 3536 Size: 93 5932 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2139 2262 2683 2842 3133 3344 Size: 399 319 30 123 421 159 291 211 Cuts at: 3344 4086 4171 5242 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 319 291 211 159 123 85 30 BspEI T'CCGG_A Cuts at: 5688 5688 Size: 6025 BspHI T'CATG_A Cuts at: 2470 3061 3914 5644 2470 Size: 591 853 1730 2851 Fragments arranged by size: 2851 1730 853 591 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2326 2380 3159 3380 844 Size: 1482 54 779 221 3489 Fragments arranged by size: 3489 1482 779 221 54 BsrBI CCG'CTC Cuts at: 1394 1823 3067 3912 1394 Size: 429 1244 845 3507 Fragments arranged by size: 3507 1244 845 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2968 4520 4694 65 Size: 1183 1720 1552 174 1396 Fragments arranged by size: 1720 1552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2384 4669 4856 1700 Size: 110 574 2285 187 2869 Fragments arranged by size: 2869 2285 574 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6025 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 4085 5097 4085 Size: 1012 5013 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6025 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6025 BstYI r'GATC_y Cuts at: 1972 2607 2803 3309 3355 3722 4107 4124 5576 Size: 635 196 506 46 367 385 17 1452 Cuts at: 5576 5662 5674 6020 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 635 506 385 367 346 196 86 46 17 12 Bsu36I CC'TnA_GG Cuts at: 3025 3025 Size: 6025 BtgI C'CryG_G Cuts at: 513 1065 2004 2062 2796 513 Size: 552 939 58 734 3742 Fragments arranged by size: 3742 939 734 552 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 3541 4335 4355 3541 Size: 794 20 5211 Fragments arranged by size: 5211 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5990 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DraIII CAC_nnn'GTG Cuts at: 2068 2678 2068 Size: 610 5415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2278 3472 4361 Size: 54 1425 50 275 18 448 1194 889 Cuts at: 4361 5990 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1194 889 448 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2886 2995 3171 3955 1353 Size: 1533 109 176 784 3423 Fragments arranged by size: 3423 1533 784 176 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3158 3202 4612 4986 5132 387 Size: 1064 138 1569 44 1410 374 146 1280 Fragments arranged by size: 1569 1410 1280 1064 374 146 138 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2189 2231 2417 2756 2826 2867 Size: 393 491 66 42 186 339 70 41 Cuts at: 2867 2880 3031 4088 4685 5472 5740 1239 Size: 13 151 1057 597 787 268 1524 Fragments arranged by size: 1524 1057 787 597 491 393 339 268 186 151 70 66 42 41 13 EcoNI CCTnn'n_nnAGG Cuts at: 2777 2777 Size: 6025 EcoO109I rG'GnC_Cy Cuts at: 2284 2770 3262 2284 Size: 486 492 5047 Fragments arranged by size: 5047 492 486 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 2025 2046 5172 1678 Size: 347 21 3126 2531 Fragments arranged by size: 3126 2531 347 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2524 2556 2599 2631 3029 Size: 32 135 32 952 32 43 32 398 Cuts at: 3029 3061 3194 3226 4666 4698 4740 4772 1373 Size: 32 133 32 1440 32 42 32 2626 Fragments arranged by size: 2626 1440 952 398 135 133 43 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3615 669 Size: 988 281 1431 246 3079 Fragments arranged by size: 3079 1431 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6025 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KasI G'GCGC_C Cuts at: 2042 2042 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5139 5323 1305 Size: 3834 184 2007 Fragments arranged by size: 3834 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4203 Fragments arranged by size: 4203 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 3983 4342 4501 518 Size: 648 1071 1746 359 159 2042 Fragments arranged by size: 2042 1746 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3014 3160 3441 4118 5266 5511 1606 Size: 1408 146 281 677 1148 245 2120 Fragments arranged by size: 2120 1408 1148 677 281 245 146 NaeI GCC'GGC Cuts at: 2386 2386 Size: 6025 NarI GG'CG_CC Cuts at: 2043 2043 Size: 6025 NcoI C'CATG_G Cuts at: 513 1065 2004 2796 513 Size: 552 939 792 3742 Fragments arranged by size: 3742 939 792 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NgoMIV G'CCGG_C Cuts at: 2384 2384 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2272 2348 3383 4928 1132 Size: 97 1043 76 1035 1545 2229 Fragments arranged by size: 2229 1545 1043 1035 97 76 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 2268 4924 2268 Size: 2656 3369 PflMI CCAn_nnn'nTGG Cuts at: 1108 2869 2973 3203 1108 Size: 1761 104 230 3930 Fragments arranged by size: 3930 1761 230 104 PfoI T'CCnGG_A Cuts at: 2433 2925 3114 2433 Size: 492 189 5344 Fragments arranged by size: 5344 492 189 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PspXI vC'TCGA_Gb Cuts at: 2140 2140 Size: 6025 PstI C_TGCA'G Cuts at: 830 2315 2781 3190 3377 830 Size: 1485 466 409 187 3478 Fragments arranged by size: 3478 1485 466 409 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 3014 3160 3014 Size: 146 5879 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SfcI C'TryA_G Cuts at: 826 1045 2311 2777 3186 3373 4508 5189 5380 Size: 219 1266 466 409 187 1135 681 191 Cuts at: 5380 826 Size: 1471 Fragments arranged by size: 1471 1266 1135 681 466 409 219 191 187 SfoI GGC'GCC Cuts at: 2044 2044 Size: 6025 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 3352 1872 Size: 1480 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2140 4129 5030 5292 820 Size: 197 926 197 1989 901 262 1553 Fragments arranged by size: 1989 1553 926 901 262 197 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 3383 1132 Size: 2251 3774 SspI AAT'ATT Cuts at: 5 52 3949 5 Size: 47 3897 2081 Fragments arranged by size: 3897 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6025 StyI C'CwwG_G Cuts at: 513 1065 2004 2796 3464 513 Size: 552 939 792 668 3074 Fragments arranged by size: 3074 939 792 668 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 4052 4069 4222 4407 4852 325 Size: 1255 702 1770 17 153 185 445 1498 Fragments arranged by size: 1770 1498 1255 702 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4271 5983 96 Size: 274 80 33 51 1443 2294 1712 138 Fragments arranged by size: 2294 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5985 6017 5985 Size: 32 5993 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6025 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XhoI C'TCGA_G Cuts at: 1943 2140 1943 Size: 197 5828 XmaI C'CCGG_G Cuts at: 1870 3350 1870 Size: 1480 4545 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI