(Circular) (Six-base) MAPSORT of: pFN21AB6587.seq Check: 7058 from: 1 to: 5770 pFN21AB6587 5770 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2648 3833 5217 5485 1632 Size: 1016 1185 1384 268 1917 Fragments arranged by size: 1917 1384 1185 1016 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 2809 4669 5646 1203 Size: 1606 1860 977 1327 Fragments arranged by size: 1860 1606 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 2152 3513 5085 2152 Size: 1361 1572 2837 Fragments arranged by size: 2837 1572 1361 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2201 3095 1870 Size: 73 87 171 894 4545 Fragments arranged by size: 4545 894 171 87 73 BamHI G'GATC_C Cuts at: 2239 2733 3100 3467 2239 Size: 494 367 367 4542 Fragments arranged by size: 4542 494 367 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3091 4546 618 Size: 325 217 627 1304 1455 1842 Fragments arranged by size: 1842 1455 1304 627 325 217 BanII G_rGCy'C Cuts at: 1391 2108 2406 3089 1391 Size: 717 298 683 4072 Fragments arranged by size: 4072 717 683 298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2184 928 Size: 971 285 4514 Fragments arranged by size: 4514 971 285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2707 2978 3662 4872 2707 Size: 271 684 1210 3605 Fragments arranged by size: 3605 1210 684 271 BclI T'GATC_A Cuts at: 1548 3049 1548 Size: 1501 4269 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2714 4381 136 Size: 107 122 71 631 605 1042 1667 1525 Fragments arranged by size: 1667 1525 1042 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 2496 3831 4987 1790 Size: 706 1335 1156 2573 Fragments arranged by size: 2573 1335 1156 706 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2686 2723 4430 1239 Size: 845 602 37 1707 2579 Fragments arranged by size: 2579 1707 845 602 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 3895 4760 5058 2486 Size: 1409 865 298 3198 Fragments arranged by size: 3198 1409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 4433 882 Size: 617 726 2208 2219 Fragments arranged by size: 2219 2208 726 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2995 3577 3959 4594 Size: 53 83 186 936 1462 582 382 635 Cuts at: 4594 275 Size: 1451 Fragments arranged by size: 1462 1451 936 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 1178 Size: 30 165 30 1104 30 4411 Fragments arranged by size: 4411 1104 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2219 2234 2383 2386 2395 1186 Size: 1018 15 15 149 3 9 4561 Fragments arranged by size: 4561 1018 149 15 15 9 3 BseYI C'CCAG_C Cuts at: 2042 2167 2339 2723 2761 2892 4973 5469 2042 Size: 125 172 384 38 131 2081 496 2343 Fragments arranged by size: 2343 2081 496 384 172 131 125 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 3831 3916 4987 2108 Size: 1723 85 1071 2891 Fragments arranged by size: 2891 1723 1071 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2406 2496 3089 3831 3916 4987 Size: 399 318 298 90 593 742 85 1071 Cuts at: 4987 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 593 399 318 298 90 85 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 3125 844 Size: 1208 1073 3489 Fragments arranged by size: 3489 1208 1073 BsrBI CCG'CTC Cuts at: 1394 1823 2458 3657 1394 Size: 429 635 1199 3507 Fragments arranged by size: 3507 1199 635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 2313 4414 4601 1700 Size: 110 311 192 2101 187 2869 Fragments arranged by size: 2869 2101 311 192 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstXI CCAn_nnnn'nTGG Cuts at: 2773 2920 2773 Size: 147 5623 BstYI r'GATC_y Cuts at: 1972 2239 2733 2884 3100 3467 3852 3869 5321 Size: 267 494 151 216 367 385 17 1452 Cuts at: 5321 5407 5419 5765 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 494 385 367 346 267 216 151 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2092 2496 513 Size: 552 939 88 404 3787 Fragments arranged by size: 3787 939 552 404 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 3286 4080 4100 3286 Size: 794 20 4956 Fragments arranged by size: 4956 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2637 2841 Size: 54 1425 50 275 18 381 426 204 Cuts at: 2841 2925 2949 3217 4106 5735 8 Size: 84 24 268 889 1629 43 Fragments arranged by size: 1629 1425 889 426 381 275 268 204 84 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2181 2286 2360 2772 3700 1353 Size: 828 105 74 412 928 3423 Fragments arranged by size: 3423 928 828 412 105 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2392 4357 4731 4877 387 Size: 1064 138 803 1965 374 146 1280 Fragments arranged by size: 1965 1280 1064 803 374 146 138 EcoICRI GAG'CTC Cuts at: 2106 2106 Size: 5770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2648 2686 2723 3833 4430 5217 Size: 393 452 564 38 37 1110 597 787 Cuts at: 5217 5485 1239 Size: 268 1524 Fragments arranged by size: 1524 1110 787 597 564 452 393 268 38 37 EcoNI CCTnn'n_nnAGG Cuts at: 2279 2978 2279 Size: 699 5071 EcoO109I rG'GnC_Cy Cuts at: 2149 2436 2758 2785 2971 2149 Size: 287 322 27 186 4948 Fragments arranged by size: 4948 322 287 186 27 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2210 2270 2210 Size: 60 5710 FspI TGC'GCA Cuts at: 2966 4276 2966 Size: 1310 4460 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 2423 2455 2507 Size: 32 135 32 652 32 167 32 52 Cuts at: 2507 2539 4411 4443 4485 4517 1373 Size: 32 1872 32 42 32 2626 Fragments arranged by size: 2626 1872 652 167 135 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2046 2244 2841 4884 5068 1305 Size: 741 198 597 2043 184 2007 Fragments arranged by size: 2043 2007 741 597 198 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2843 2927 2951 10 Size: 54 1425 343 1011 84 24 2829 Fragments arranged by size: 2829 1425 1011 343 84 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2348 2489 2675 3728 4087 4246 518 Size: 648 1182 141 186 1053 359 159 2042 Fragments arranged by size: 2042 1182 1053 648 359 186 159 141 MspA1I CmG'CkG Cuts at: 1606 2498 3186 3863 5011 5256 1606 Size: 892 688 677 1148 245 2120 Fragments arranged by size: 2120 1148 892 688 677 245 NaeI GCC'GGC Cuts at: 2123 2315 2123 Size: 192 5578 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NgoMIV G'CCGG_C Cuts at: 2121 2313 2121 Size: 192 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2674 2813 3128 4673 1132 Size: 97 1445 139 315 1545 2229 Fragments arranged by size: 2229 1545 1445 315 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 2809 4669 2809 Size: 1860 3910 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 1108 Size: 1044 4726 PfoI T'CCnGG_A Cuts at: 2226 2274 2226 Size: 48 5722 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2785 2149 Size: 636 5134 PshAI GACnn'nnGTC Cuts at: 1613 2576 2994 1613 Size: 963 418 4389 Fragments arranged by size: 4389 963 418 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 3122 830 Size: 2292 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 SacI G_AGCT'C Cuts at: 2108 2108 Size: 5770 SacII CC_GC'GG Cuts at: 2499 2499 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SanDI GG'GwC_CC Cuts at: 2785 2785 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SfcI C'TryA_G Cuts at: 826 1045 3118 4253 4934 5125 826 Size: 219 2073 1135 681 191 1471 Fragments arranged by size: 2073 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 3874 4775 5037 820 Size: 197 926 522 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 522 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 2674 3128 1132 Size: 1542 454 3774 Fragments arranged by size: 3774 1542 454 SspI AAT'ATT Cuts at: 5 52 2552 3694 5 Size: 47 2500 1142 2081 Fragments arranged by size: 2500 2081 1142 47 StuI AGG'CCT Cuts at: 1517 3061 1517 Size: 1544 4226 StyI C'CwwG_G Cuts at: 513 1065 2004 3209 513 Size: 552 939 1205 3074 Fragments arranged by size: 3074 1205 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 2583 2895 3797 3814 3967 4152 Size: 1255 888 115 312 902 17 153 185 Cuts at: 4152 4597 325 Size: 445 1498 Fragments arranged by size: 1498 1255 902 888 445 312 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 4016 5728 Size: 274 80 33 51 1443 468 1571 1712 Cuts at: 5728 96 Size: 138 Fragments arranged by size: 1712 1571 1443 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2920 2920 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI