(Circular) (Six-base) MAPSORT of: pFN21AB6583.seq Check: 2437 from: 1 to: 5836 pFN21AB6583 5836 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3073 3073 Size: 5836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2919 3646 278 Size: 53 83 186 936 1383 727 2468 Fragments arranged by size: 2468 1383 936 727 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3899 5283 5551 1632 Size: 2267 1384 268 1917 Fragments arranged by size: 2267 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 2099 2258 1166 Size: 933 159 4744 Fragments arranged by size: 4744 933 159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2530 3579 5151 2530 Size: 1049 1572 3215 Fragments arranged by size: 3215 1572 1049 ApaI G_GGCC'C Cuts at: 2249 2888 2249 Size: 639 5197 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2116 3145 3371 3523 5632 1493 Size: 623 1029 226 152 2109 1697 Fragments arranged by size: 2109 1697 1029 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2121 2852 3104 3161 1870 Size: 73 133 45 731 252 57 4545 Fragments arranged by size: 4545 731 252 133 73 57 45 AvrII C'CTAG_G Cuts at: 3031 3031 Size: 5836 BamHI G'GATC_C Cuts at: 2610 3166 3533 2610 Size: 556 367 4913 Fragments arranged by size: 4913 556 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 3110 3157 4612 618 Size: 325 217 627 546 777 47 1455 1842 Fragments arranged by size: 1842 1455 777 627 546 325 217 47 BanII G_rGCy'C Cuts at: 1391 2138 2249 2861 2888 3155 1391 Size: 747 111 612 27 267 4072 Fragments arranged by size: 4072 747 612 267 111 27 BbeI G_GCGC'C Cuts at: 2337 3114 2337 Size: 777 5059 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2856 4447 136 Size: 107 122 71 631 605 1184 1591 1525 Fragments arranged by size: 1591 1525 1184 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2219 2249 2888 3897 5053 1790 Size: 429 30 639 1009 1156 2573 Fragments arranged by size: 2573 1156 1009 639 429 30 BmgBI CAC'GTC Cuts at: 1318 2820 1318 Size: 1502 4334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2729 3567 4525 Size: 416 53 83 399 24 1312 838 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1312 958 838 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 2459 2636 2740 4496 1239 Size: 798 422 177 104 1756 2579 Fragments arranged by size: 2579 1756 798 422 177 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2624 3961 4826 5124 2624 Size: 1337 865 298 3336 Fragments arranged by size: 3336 1337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2462 4499 882 Size: 617 963 2037 2219 Fragments arranged by size: 2219 2037 963 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2334 2916 3111 Size: 53 83 186 936 476 325 582 195 Cuts at: 3111 3643 4025 4660 275 Size: 532 382 635 1451 Fragments arranged by size: 1451 936 635 582 532 476 382 325 195 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5611 Fragments arranged by size: 5611 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2966 2969 2996 3005 3035 1186 Size: 1780 3 27 9 30 3987 Fragments arranged by size: 3987 1780 30 27 9 3 BseYI C'CCAG_C Cuts at: 2248 2881 5039 5535 2248 Size: 633 2158 496 2549 Fragments arranged by size: 2549 2158 633 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3059 1163 Size: 573 1323 3940 Fragments arranged by size: 3940 1323 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2419 2769 3286 4047 5075 1540 Size: 275 186 418 350 517 761 1028 2301 Fragments arranged by size: 2301 1028 761 517 418 350 275 186 BsiHKAI G_wGCw'C Cuts at: 3897 3982 5053 3897 Size: 85 1071 4680 Fragments arranged by size: 4680 1071 85 BsmI GAATG_Cn' Cuts at: 2156 3347 3440 2156 Size: 1191 93 4552 Fragments arranged by size: 4552 1191 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2219 2249 2861 2888 3155 3897 Size: 399 348 81 30 612 27 267 742 Cuts at: 3897 3982 5053 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 612 399 348 267 85 81 30 27 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 3073 3191 844 Size: 1559 670 118 3489 Fragments arranged by size: 3489 1559 670 118 BsrBI CCG'CTC Cuts at: 1394 1823 3130 3723 1394 Size: 429 1307 593 3507 Fragments arranged by size: 3507 1307 593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2358 4331 4505 65 Size: 1183 1110 1973 174 1396 Fragments arranged by size: 1973 1396 1183 1110 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2453 4480 4667 1700 Size: 110 643 2027 187 2869 Fragments arranged by size: 2869 2027 643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 2570 3896 4908 2570 Size: 1326 1012 3498 Fragments arranged by size: 3498 1326 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2360 794 Size: 1566 4270 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 2866 1184 Size: 1682 4154 BstXI CCAn_nnnn'nTGG Cuts at: 2260 2350 2260 Size: 90 5746 BstYI r'GATC_y Cuts at: 1972 2553 2610 2715 2730 3166 3533 3918 3935 Size: 581 57 105 15 436 367 385 17 Cuts at: 3935 5387 5473 5485 5831 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 581 436 385 367 346 105 86 57 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2862 513 Size: 552 939 90 768 3487 Fragments arranged by size: 3487 939 768 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2106 2217 3352 4146 4166 2106 Size: 111 1135 794 20 3776 Fragments arranged by size: 3776 1135 794 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 2676 3140 2676 Size: 464 5372 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 2416 2577 Size: 54 1425 50 275 18 510 76 161 Cuts at: 2577 2766 3283 4172 5801 8 Size: 189 517 889 1629 43 Fragments arranged by size: 1629 1425 889 517 510 275 189 161 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2416 2766 3283 1537 Size: 275 604 350 517 4090 Fragments arranged by size: 4090 604 517 350 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2181 2954 3766 1353 Size: 828 773 812 3423 Fragments arranged by size: 3423 828 812 773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 4423 4797 4943 387 Size: 1064 138 528 2306 374 146 1280 Fragments arranged by size: 2306 1280 1064 528 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2459 2636 2740 3899 4496 5283 Size: 393 405 422 177 104 1159 597 787 Cuts at: 5283 5551 1239 Size: 268 1524 Fragments arranged by size: 1524 1159 787 597 422 405 393 268 177 104 EcoO109I rG'GnC_Cy Cuts at: 2885 2885 Size: 5836 EcoRI G'AATT_C Cuts at: 2116 3145 3523 2116 Size: 1029 378 4429 Fragments arranged by size: 4429 1029 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2687 2687 Size: 5836 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2337 3114 4983 1678 Size: 659 777 1869 2531 Fragments arranged by size: 2531 1869 777 659 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 2479 2511 2804 Size: 32 135 32 650 32 225 32 293 Cuts at: 2804 2836 4477 4509 4551 4583 1373 Size: 32 1641 32 42 32 2626 Fragments arranged by size: 2626 1641 650 293 225 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KasI G'GCGC_C Cuts at: 2333 3110 2333 Size: 777 5059 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 10 Size: 54 1425 343 510 3504 Fragments arranged by size: 3504 1425 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2258 2348 3794 4153 4312 518 Size: 648 933 159 90 1446 359 159 2042 Fragments arranged by size: 2042 1446 933 648 359 159 159 90 MspA1I CmG'CkG Cuts at: 1606 2086 2437 2582 3252 3929 5077 5322 1606 Size: 480 351 145 670 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 670 480 351 245 145 NarI GG'CG_CC Cuts at: 2334 3111 2334 Size: 777 5059 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2575 2607 2881 1108 Size: 1467 32 274 4063 Fragments arranged by size: 4063 1467 274 32 PfoI T'CCnGG_A Cuts at: 2053 2523 2556 2613 2053 Size: 470 33 57 5276 Fragments arranged by size: 5276 470 57 33 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2245 2884 2245 Size: 639 5197 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2437 2437 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2111 3184 4319 5000 5191 826 Size: 219 1066 1073 1135 681 191 1471 Fragments arranged by size: 1471 1135 1073 1066 681 219 191 SfoI GGC'GCC Cuts at: 2335 3112 2335 Size: 777 5059 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 2123 3163 1872 Size: 251 1040 4545 Fragments arranged by size: 4545 1040 251 SmlI C'TyrA_G Cuts at: 820 1017 1943 2076 2639 3940 4841 5103 820 Size: 197 926 133 563 1301 901 262 1553 Fragments arranged by size: 1553 1301 926 901 563 262 197 133 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2586 3031 3275 513 Size: 552 939 237 345 445 244 3074 Fragments arranged by size: 3074 939 552 445 345 244 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5796 5828 5796 Size: 32 5804 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2611 2611 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 2076 1943 Size: 133 5703 XmaI C'CCGG_G Cuts at: 1870 2121 3161 1870 Size: 251 1040 4545 Fragments arranged by size: 4545 1040 251 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2917 3644 276 Size: 53 83 186 936 1383 727 2468 Fragments arranged by size: 2468 1383 936 727 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI