Restriction map for
pFN21AB6570
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI Hin1I HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI SwaI TfiI
Tsp509I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 421 GTCTAC GT^[AC][GT]AC
AflIII 1010 ACACGT A^C[GA][CT]GT
AlwI 255 GATCC GGATC(4/5)
AlwNI 471 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 607 AGCGCT AGC^GCT
ApaI 918 GGGCCC GGGCC^C
Asp700I 345 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 562 CCCGAG C^[CT]CG[GA]G
BalI 1073 TGGCCA TGG^CCA
BanI 696 GGTGCC G^G[CT][GA]CC
BbrPI 331 CACGTG CAC^GTG
BfaI 400 CTAG C^TAG
BlpI 1054 GCTGAGC GC^T[ACGT]AGC
BpmI 309 CTCCAG CTGGAG(16/14)
Bpu1102I 1054 GCTGAGC GC^T[ACGT]AGC
BsaAI 331 CACGTG [CT]AC^GT[GA]
BsaI 658 GAGACC GGTCTC(1/5)
BsaWI 188 ACCGGA [AT]^CCGG[AT]
BsgI 127 CTGCAC GTGCAG(16/14)
BsoBI 562 CCCGAG C^[CT]CG[GA]G
Bsp1407I 34 TGTACA T^GTACA
BsrGI 34 TGTACA T^GTACA
BssHII 703 GCGCGC G^CGCGC
BstAPI 470 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 254 AGATCC [GA]^GATC[CT]
CelII 1054 GCTGAGC GC^T[ACGT]AGC
DraIII 856 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 85 GAAGAG CTCTTC(1/4)
Eco47III 607 AGCGCT AGC^GCT
FseI 1037 GGCCGGCC GGCCGG^CC
HincII 46 GTCAAC GT[CT]^[GA]AC
HindII 46 GTCAAC GT[CT]^[GA]AC
Ksp632I 85 GAAGAG CTCTTC(1/4)
KspI 229 CCGCGG CCGC^GG
MaeI 400 CTAG C^TAG
MflI 254 AGATCC [GA]^GATC[CT]
MluNI 1073 TGGCCA TGG^CCA
MscI 1073 TGGCCA TGG^CCA
MseI 142 TTAA T^TAA
NaeI 1038 GCCGGC GCC^GGC
NcoI 1076 CCATGG C^CATGG
NgoMIV 1038 GCCGGC G^CCGGC
NspI 641 GCATGC [GA]CATG^[CT]
PmaCI 331 CACGTG CAC^GTG
PmlI 331 CACGTG CAC^GTG
SacII 229 CCGCGG CCGC^GG
SapI 85 GAAGAGC GCTCTTC(1/4)
ScaI 65 AGTACT AGT^ACT
SexAI 857 ACCTGGT A^CC[AT]GGT
SphI 641 GCATGC GCATG^C
SspBI 34 TGTACA T^GTACA
StuI 239 AGGCCT AGG^CCT
Tru9I 142 TTAA T^TAA
XhoII 254 AGATCC [GA]^GATC[CT]
XmnI 345 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 328 GTGCAC G^TGCAC
862 GTGCAC
ApaLI 328 GTGCAC G^TGCAC
862 GTGCAC
BanII 462 GGGCTC G[GA]GC[CT]^C
918 GGGCCC
BbvCI 315 CCTCAGC CCTCAGC(-5/-2)
377 CCTCAGC
BsmFI 296 GGGAC GGGAC(10/14)
887 GGGAC
BspMI 796 ACCTGC ACCTGC(4/8)
1029 GCAGGT
BsrFI 42 GCCGGT [GA]^CCGG[CT]
1038 GCCGGC
Cfr10I 42 GCCGGT [GA]^CCGG[CT]
1038 GCCGGC
DpnII 255 GATC ^GATC
933 GATC
EaeI 204 TGGCCG [CT]^GGCC[GA]
1073 TGGCCA
EcoT14I 893 CCAAGG C^C[AT][AT]GG
1076 CCATGG
HgaI 913 GACGC GACGC(5/10)
119 GCGTC
HinfI 565 GAGTC G^A[ACGT]TC
688 GAGTC
MaeII 332 ACGT A^CGT
1012 ACGT
MboI 255 GATC ^GATC
933 GATC
NdeII 255 GATC ^GATC
933 GATC
PflMI 836 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCACCTCCTGG
PleI 565 GAGTC GAGTC(4/5)
688 GAGTC
PpuMI 296 GGGACCC [GA]G^G[AT]CC[CT]
1170 GGGTCCT
PstI 235 CTGCAG CTGCA^G
1027 CTGCAG
Sau3AI 255 GATC ^GATC
933 GATC
SfcI 235 CTGCAG C^T[GA][CT]AG
1027 CTGCAG
StyI 893 CCAAGG C^C[AT][AT]GG
1076 CCATGG
TaqI 803 TCGA T^CGA
1000 TCGA
TthHB8I 803 TCGA T^CGA
1000 TCGA
Van91I 836 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCACCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BbvI BcnI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PvuII RsaI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI