(Circular) (Six-base) MAPSORT of: pFN21AB6570.seq Check: 5136 from: 1 to: 5905 pFN21AB6570 5905 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2810 3025 2810 Size: 215 5690 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2427 3248 1937 Size: 490 821 4594 Fragments arranged by size: 4594 821 490 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 3050 3968 5352 5620 1632 Size: 1412 6 918 1384 268 1917 Fragments arranged by size: 1917 1412 1384 918 268 6 AfeI AGC'GCT Cuts at: 2614 2614 Size: 5905 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 3015 4804 5781 1203 Size: 1812 1789 977 1327 Fragments arranged by size: 1812 1789 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 2900 3125 1166 Size: 1734 225 3946 Fragments arranged by size: 3946 1734 225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2481 3648 5220 2481 Size: 1167 1572 3166 Fragments arranged by size: 3166 1572 1167 ApaI G_GGCC'C Cuts at: 2927 2927 Size: 5905 ApaLI G'TGCA_C Cuts at: 2333 2867 3962 5118 2333 Size: 534 1095 1156 3120 Fragments arranged by size: 3120 1156 1095 534 ApoI r'AATT_y Cuts at: 1493 3214 3440 3592 5701 1493 Size: 1721 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2567 3230 1870 Size: 73 624 663 4545 Fragments arranged by size: 4545 663 624 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2701 3226 4681 618 Size: 325 217 627 914 525 1455 1842 Fragments arranged by size: 1842 1455 914 627 525 325 217 BanII G_rGCy'C Cuts at: 1391 2471 2927 3224 1391 Size: 1080 456 297 4072 Fragments arranged by size: 4072 1080 456 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4934 BbvCI CC'TCA_GC Cuts at: 2321 2383 2321 Size: 62 5843 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3060 3322 3060 Size: 262 5643 Bme1580I G_kGCm'C Cuts at: 1790 2337 2706 2871 2927 3966 5122 1790 Size: 547 369 165 56 1039 1156 2573 Fragments arranged by size: 2573 1156 1039 547 369 165 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 3636 4594 Size: 416 53 83 399 24 666 1553 958 Cuts at: 4594 442 Size: 1753 Fragments arranged by size: 1753 1553 958 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2145 2145 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2299 4565 1239 Size: 1060 2266 2579 Fragments arranged by size: 2579 2266 1060 Bpu10I CC'TnA_GC Cuts at: 2120 2321 2383 2470 2120 Size: 201 62 87 5555 Fragments arranged by size: 5555 201 87 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2657 4568 882 Size: 617 1158 1911 2219 Fragments arranged by size: 2219 1911 1158 617 BsaAI yAC'GTr Cuts at: 493 1204 2338 3696 493 Size: 711 1134 1358 2702 Fragments arranged by size: 2702 1358 1134 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3712 4094 4729 275 Size: 53 83 186 936 2179 382 635 1451 Fragments arranged by size: 2179 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2193 4338 4736 5010 5157 5568 2193 Size: 2145 398 274 147 411 2530 Fragments arranged by size: 2530 2145 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2104 2231 2371 1186 Size: 918 127 140 4720 Fragments arranged by size: 4720 918 140 127 BseYI C'CCAG_C Cuts at: 2753 2763 5108 5604 2753 Size: 10 2345 496 3054 Fragments arranged by size: 3054 2345 496 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 1163 Size: 573 381 4951 Fragments arranged by size: 4951 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 2598 2871 3966 4051 5122 2337 Size: 261 273 1095 85 1071 3120 Fragments arranged by size: 3120 1095 1071 273 261 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2471 2598 2706 2871 2927 3224 Size: 399 547 134 127 108 165 56 297 Cuts at: 3224 3966 4051 5122 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 547 399 297 165 134 127 108 85 56 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 3025 3260 844 Size: 1966 215 235 3489 Fragments arranged by size: 3489 1966 235 215 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2047 3043 4549 4736 1700 Size: 110 237 996 1506 187 2869 Fragments arranged by size: 2869 1506 996 237 187 110 BsrGI T'GTAC_A Cuts at: 96 2039 96 Size: 1943 3962 BssHII G'CGCG_C Cuts at: 1925 2708 1925 Size: 783 5122 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 2481 794 Size: 1687 4218 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2259 3235 3602 3987 4004 5456 5542 5554 Size: 287 976 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 976 385 367 346 287 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2234 2361 2640 2697 2887 3081 Size: 552 939 230 127 279 57 190 194 Cuts at: 3081 513 Size: 3337 Fragments arranged by size: 3337 939 552 279 230 194 190 127 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2041 2100 2533 487 Size: 683 56 815 59 433 3859 Fragments arranged by size: 3859 815 683 433 59 56 BtsI GCAGTG_nn' Cuts at: 3421 4215 4235 3421 Size: 794 20 5091 Fragments arranged by size: 5091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DraIII CAC_nnn'GTG Cuts at: 2866 2866 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 3078 3352 Size: 54 1425 50 275 18 379 869 274 Cuts at: 3352 4241 5870 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 869 379 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 3835 1353 Size: 732 1750 3423 Fragments arranged by size: 3423 1750 732 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2732 4492 4866 5012 387 Size: 1064 138 1143 1760 374 146 1280 Fragments arranged by size: 1760 1280 1143 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 3044 3050 3968 4565 5352 5620 Size: 393 667 745 6 918 597 787 268 Cuts at: 5620 1239 Size: 1524 Fragments arranged by size: 1524 918 787 745 667 597 393 268 6 EcoO109I rG'GnC_Cy Cuts at: 2302 2924 3137 3176 2302 Size: 622 213 39 5031 Fragments arranged by size: 5031 622 213 39 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FseI GG_CCGG'CC Cuts at: 3047 3047 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2048 2481 2616 5052 1678 Size: 370 433 135 2436 2531 Fragments arranged by size: 2531 2436 433 370 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373 Size: 32 135 32 2974 32 42 32 2626 Fragments arranged by size: 2974 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 3249 3495 669 Size: 988 281 115 1196 246 3079 Fragments arranged by size: 3079 1196 988 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5019 5203 1305 Size: 3714 184 2007 Fragments arranged by size: 3714 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3080 10 Size: 54 1425 343 1248 2835 Fragments arranged by size: 2835 1425 1248 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 2900 3125 3863 4222 4381 518 Size: 648 1437 297 225 738 359 159 2042 Fragments arranged by size: 2042 1437 738 648 359 297 225 159 MspA1I CmG'CkG Cuts at: 1606 2023 2236 2265 2308 2325 2387 2730 2767 Size: 417 213 29 43 17 62 343 37 Cuts at: 2767 3059 3200 3321 3998 5146 5391 1606 Size: 292 141 121 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 417 343 292 245 213 141 121 62 43 37 29 17 NaeI GCC'GGC Cuts at: 3045 3045 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 2004 3081 513 Size: 552 939 1077 3337 Fragments arranged by size: 3337 1077 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NgoMIV G'CCGG_C Cuts at: 3043 3043 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2650 3263 4808 1132 Size: 97 1421 613 1545 2229 Fragments arranged by size: 2229 1545 1421 613 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 2847 3172 1108 Size: 1739 325 3841 Fragments arranged by size: 3841 1739 325 PfoI T'CCnGG_A Cuts at: 2736 3147 2736 Size: 411 5494 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PmlI CAC'GTG Cuts at: 2338 2338 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2302 3176 2302 Size: 874 5031 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2923 2923 Size: 5905 PstI C_TGCA'G Cuts at: 830 2244 3036 3257 830 Size: 1414 792 221 3478 Fragments arranged by size: 3478 1414 792 221 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2325 2767 3059 3200 2325 Size: 442 292 141 5030 Fragments arranged by size: 5030 442 292 141 SacII CC_GC'GG Cuts at: 2237 2237 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SanDI GG'GwC_CC Cuts at: 2302 2302 Size: 5905 SapI GCTCTTCn'nnn_ Cuts at: 2085 2085 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 2072 4153 5865 2072 Size: 2081 1712 2112 Fragments arranged by size: 2112 2081 1712 SexAI A'CCwGG_T Cuts at: 2862 2862 Size: 5905 SfcI C'TryA_G Cuts at: 826 1045 2240 3032 3253 4388 5069 5260 826 Size: 219 1195 792 221 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 792 681 221 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 2650 3263 1132 Size: 1518 613 3774 Fragments arranged by size: 3774 1518 613 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 2246 1517 Size: 729 5176 StyI C'CwwG_G Cuts at: 513 1065 2004 2898 3081 3344 513 Size: 552 939 894 183 263 3074 Fragments arranged by size: 3074 939 894 552 263 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3932 3949 4102 4287 4732 325 Size: 1255 2352 17 153 185 445 1498 Fragments arranged by size: 2352 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2039 2070 4151 Size: 274 80 33 51 1443 62 31 2081 Cuts at: 4151 5863 96 Size: 1712 138 Fragments arranged by size: 2081 1712 1443 274 138 80 62 51 33 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 2354 4034 2354 Size: 1680 4225 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SfiI SfoI SrfI SwaI XcmI