Restriction map for
pFN21AB6570
(Linear) (Six-base) MAPSORT of: KIBB6570.seq Check: 2325 from: 1 to: 1200
KIBB6570.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 805 1020 1200
Size: 805 215 180
Fragments arranged by size:
805 215 180
AccI GT'mk_AC
Cuts at: 0 422 1200
Size: 422 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1045 1200
Size: 1039 6 155
Fragments arranged by size:
1039 155 6
AfeI AGC'GCT
Cuts at: 0 609 1200
Size: 609 591
AflIII A'CryG_T
Cuts at: 0 1010 1200
Size: 1010 190
AleI CACnn'nnGTG
Cuts at: 0 895 1120 1200
Size: 895 225 80
Fragments arranged by size:
895 225 80
AlwNI CAG_nnn'CTG
Cuts at: 0 476 1200
Size: 476 724
ApaI G_GGCC'C
Cuts at: 0 922 1200
Size: 922 278
ApaLI G'TGCA_C
Cuts at: 0 328 862 1200
Size: 328 534 338
Fragments arranged by size:
534 338 328
AvaI C'yCGr_G
Cuts at: 0 562 1200
Size: 562 638
BanI G'GyrC_C
Cuts at: 0 696 1200
Size: 696 504
BanII G_rGCy'C
Cuts at: 0 466 922 1200
Size: 466 456 278
Fragments arranged by size:
466 456 278
BbvCI CC'TCA_GC
Cuts at: 0 316 378 1200
Size: 316 62 822
Fragments arranged by size:
822 316 62
BlpI GC'TnA_GC
Cuts at: 0 1055 1200
Size: 1055 145
Bme1580I G_kGCm'C
Cuts at: 0 332 701 866 922 1200
Size: 332 369 165 56 278
Fragments arranged by size:
369 332 278 165 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 78 1200
Size: 78 1122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 140 1200
Size: 140 1060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1200
Size: 294 906
Bpu10I CC'TnA_GC
Cuts at: 0 115 316 378 465 1200
Size: 115 201 62 87 735
Fragments arranged by size:
735 201 115 87 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 652 1200
Size: 652 548
BsaAI yAC'GTr
Cuts at: 0 333 1200
Size: 333 867
BsaWI w'CCGG_w
Cuts at: 0 188 1200
Size: 188 1012
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 226 366 1200
Size: 99 127 140 834
Fragments arranged by size:
834 140 127 99
BseYI C'CCAG_C
Cuts at: 0 748 758 1200
Size: 748 10 442
Fragments arranged by size:
748 442 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1200
Size: 112 1088
BsiHKAI G_wGCw'C
Cuts at: 0 332 593 866 1200
Size: 332 261 273 334
Fragments arranged by size:
334 332 273 261
Bsp1286I G_dGCh'C
Cuts at: 0 332 466 593 701 866 922 1200
Size: 332 134 127 108 165 56 278
Fragments arranged by size:
332 278 165 134 127 108 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 805 1020 1200
Size: 805 215 180
Fragments arranged by size:
805 215 180
BsrFI r'CCGG_y
Cuts at: 0 42 1038 1200
Size: 42 996 162
Fragments arranged by size:
996 162 42
BsrGI T'GTAC_A
Cuts at: 0 34 1200
Size: 34 1166
BssHII G'CGCG_C
Cuts at: 0 703 1200
Size: 703 497
BstAPI GCAn_nnn'nTGC
Cuts at: 0 476 1200
Size: 476 724
BstYI r'GATC_y
Cuts at: 0 254 1200
Size: 254 946
BtgI C'CryG_G
Cuts at: 0 229 356 635 692 882 1076 1200
Size: 229 127 279 57 190 194 124
Fragments arranged by size:
279 229 194 190 127 124 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 95 528 1200
Size: 36 59 433 672
Fragments arranged by size:
672 433 59 36
DraIII CAC_nnn'GTG
Cuts at: 0 861 1200
Size: 861 339
EaeI y'GGCC_r
Cuts at: 0 204 1073 1200
Size: 204 869 127
Fragments arranged by size:
869 204 127
EarI CTCTTCn'nnn_
Cuts at: 0 80 1200
Size: 80 1120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 727 1200
Size: 727 473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1039 1045 1200
Size: 294 745 6 155
Fragments arranged by size:
745 294 155 6
EcoO109I rG'GnC_Cy
Cuts at: 0 297 919 1132 1171 1200
Size: 297 622 213 39 29
Fragments arranged by size:
622 297 213 39 29
FseI GG_CCGG'CC
Cuts at: 0 1042 1200
Size: 1042 158
HaeII r_GCGC'y
Cuts at: 0 43 476 611 1200
Size: 43 433 135 589
Fragments arranged by size:
589 433 135 43
HincII GTy'rAC
Cuts at: 0 48 1200
Size: 48 1152
MscI TGG'CCA
Cuts at: 0 1075 1200
Size: 1075 125
MslI CAynn'nnrTG
Cuts at: 0 598 895 1120 1200
Size: 598 297 225 80
Fragments arranged by size:
598 297 225 80
MspA1I CmG'CkG
Cuts at: 0 18 231 260 303 320 382 725 762
Size: 18 213 29 43 17 62 343 37
Cuts at: 762 1054 1195 1200
Size: 292 141 5
Fragments arranged by size:
343 292 213 141 62 43 37 29
18 17 5
NaeI GCC'GGC
Cuts at: 0 1040 1200
Size: 1040 160
NcoI C'CATG_G
Cuts at: 0 1076 1200
Size: 1076 124
NgoMIV G'CCGG_C
Cuts at: 0 1038 1200
Size: 1038 162
NspI r_CATG'y
Cuts at: 0 645 1200
Size: 645 555
PflMI CCAn_nnn'nTGG
Cuts at: 0 842 1167 1200
Size: 842 325 33
Fragments arranged by size:
842 325 33
PfoI T'CCnGG_A
Cuts at: 0 731 1142 1200
Size: 731 411 58
Fragments arranged by size:
731 411 58
PmlI CAC'GTG
Cuts at: 0 333 1200
Size: 333 867
PpuMI rG'GwC_Cy
Cuts at: 0 297 1171 1200
Size: 297 874 29
Fragments arranged by size:
874 297 29
PspOMI G'GGCC_C
Cuts at: 0 918 1200
Size: 918 282
PstI C_TGCA'G
Cuts at: 0 239 1031 1200
Size: 239 792 169
Fragments arranged by size:
792 239 169
PvuII CAG'CTG
Cuts at: 0 320 762 1054 1195 1200
Size: 320 442 292 141 5
Fragments arranged by size:
442 320 292 141 5
SacII CC_GC'GG
Cuts at: 0 232 1200
Size: 232 968
SanDI GG'GwC_CC
Cuts at: 0 297 1200
Size: 297 903
SapI GCTCTTCn'nnn_
Cuts at: 0 80 1200
Size: 80 1120
ScaI AGT'ACT
Cuts at: 0 67 1200
Size: 67 1133
SexAI A'CCwGG_T
Cuts at: 0 857 1200
Size: 857 343
SfcI C'TryA_G
Cuts at: 0 235 1027 1200
Size: 235 792 173
Fragments arranged by size:
792 235 173
SphI G_CATG'C
Cuts at: 0 645 1200
Size: 645 555
StuI AGG'CCT
Cuts at: 0 241 1200
Size: 241 959
StyI C'CwwG_G
Cuts at: 0 893 1076 1200
Size: 893 183 124
Fragments arranged by size:
893 183 124
TatI w'GTAC_w
Cuts at: 0 34 65 1200
Size: 34 31 1135
Fragments arranged by size:
1135 34 31
XmnI GAAnn'nnTTC
Cuts at: 0 349 1200
Size: 349 851
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbvCI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssHII BstAPI
BstYI BtgI BtgZI DraIII EaeI EarI EciI Eco57MI
EcoO109I FseI HaeII HincII MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacII SanDI SapI ScaI SexAI SfcI
SphI StuI StyI TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmtI BpuEI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SbfI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI