(Circular) (Six-base) MAPSORT of: pFN21AB6553.seq Check: 5093 from: 1 to: 5818 pFN21AB6553 5818 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3881 5265 5533 1632 Size: 2249 1384 268 1917 Fragments arranged by size: 2249 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 2227 2787 4717 5694 1203 Size: 1024 560 1930 977 1327 Fragments arranged by size: 1930 1327 1024 977 560 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AlwNI CAG_nnn'CTG Cuts at: 2798 3561 5133 2798 Size: 763 1572 3483 Fragments arranged by size: 3483 1572 763 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 3127 3353 3505 5614 1493 Size: 1634 226 152 2109 1697 Fragments arranged by size: 2109 1697 1634 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2097 2115 3033 3143 1870 Size: 73 154 18 918 110 4545 Fragments arranged by size: 4545 918 154 110 73 18 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 2650 2713 2742 2857 Size: 325 217 627 431 432 63 29 115 Cuts at: 2857 2902 2917 3139 4594 618 Size: 45 15 222 1455 1842 Fragments arranged by size: 1842 1455 627 432 431 325 222 217 115 63 45 29 15 BanII G_rGCy'C Cuts at: 1391 2467 2601 2785 3137 1391 Size: 1076 134 184 352 4072 Fragments arranged by size: 4072 1076 352 184 134 BbeI G_GCGC'C Cuts at: 2746 2921 2746 Size: 175 5643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 928 Size: 971 325 4522 Fragments arranged by size: 4522 971 325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4009 4043 1903 Size: 34 50 34 1988 34 3678 Fragments arranged by size: 3678 1988 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BfrBI ATG'CAT Cuts at: 2981 2981 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2208 2261 2303 Size: 107 122 71 631 605 536 53 42 Cuts at: 2303 2740 2763 4429 136 Size: 437 23 1666 1525 Fragments arranged by size: 1666 1525 631 605 536 437 122 107 71 53 42 23 BglII A'GATC_T Cuts at: 5813 5813 Size: 5818 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 2221 2860 3034 3879 5035 1790 Size: 431 639 174 845 1156 2573 Fragments arranged by size: 2573 1156 845 639 431 174 BmgBI CAC'GTC Cuts at: 1318 2230 1318 Size: 912 4906 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2224 3549 4507 Size: 416 53 83 399 24 807 1325 958 Cuts at: 4507 442 Size: 1753 Fragments arranged by size: 1753 1325 958 807 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2114 1056 Size: 1058 4760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4478 1239 Size: 3239 2579 Bpu10I CC'TnA_GC Cuts at: 2162 2162 Size: 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4481 882 Size: 617 2982 2219 Fragments arranged by size: 2982 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2788 3609 493 Size: 711 1584 821 2702 Fragments arranged by size: 2702 1584 821 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2743 2755 2918 Size: 53 83 186 936 482 728 12 163 Cuts at: 2918 3625 4007 4642 275 Size: 707 382 635 1451 Fragments arranged by size: 1451 936 728 707 635 482 382 186 163 83 53 12 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2772 2802 1178 Size: 30 165 30 1369 30 4194 Fragments arranged by size: 4194 1369 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2371 3082 1186 Size: 1185 711 3922 Fragments arranged by size: 3922 1185 711 BseYI C'CCAG_C Cuts at: 2054 2063 2289 2523 2541 2580 2853 3050 5021 Size: 9 226 234 18 39 273 197 1971 Cuts at: 5021 5517 2054 Size: 496 2355 Fragments arranged by size: 2355 1971 496 273 234 226 197 39 18 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2894 2944 3061 1163 Size: 573 1158 50 117 3920 Fragments arranged by size: 3920 1158 573 117 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2314 2480 3268 4029 5057 1540 Size: 275 186 313 166 788 761 1028 2301 Fragments arranged by size: 2301 1028 788 761 313 275 186 166 BsiHKAI G_wGCw'C Cuts at: 2685 3879 3964 5035 2685 Size: 1194 85 1071 3468 Fragments arranged by size: 3468 1194 1071 85 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2221 2467 2601 2685 2785 2860 3034 Size: 399 431 246 134 84 100 75 174 Cuts at: 3034 3137 3879 3964 5035 1391 Size: 103 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 431 399 246 174 134 103 100 85 84 75 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 844 Size: 2329 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3705 1394 Size: 429 1882 3507 Fragments arranged by size: 3507 1882 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2173 4313 4487 65 Size: 1183 925 2140 174 1396 Fragments arranged by size: 2140 1396 1183 925 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2202 2475 2479 4462 4649 1700 Size: 110 392 273 4 1983 187 2869 Fragments arranged by size: 2869 1983 392 273 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5818 BssSI C'ACGA_G Cuts at: 3878 4890 3878 Size: 1012 4806 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2407 2459 2011 Size: 396 52 5370 Fragments arranged by size: 5370 396 52 BstYI r'GATC_y Cuts at: 1972 2973 3148 3515 3900 3917 5369 5455 5467 Size: 1001 175 367 385 17 1452 86 12 Cuts at: 5467 5813 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1001 385 367 346 175 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2265 2320 2452 513 Size: 552 1200 55 132 3879 Fragments arranged by size: 3879 1200 552 132 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2253 2262 Size: 54 1425 50 275 18 409 14 9 Cuts at: 2262 2397 2477 2802 2943 3099 3265 4154 5783 Size: 135 80 325 141 156 166 889 1629 Cuts at: 5783 8 Size: 43 Fragments arranged by size: 1629 1425 889 409 325 275 166 156 141 135 80 54 50 43 18 14 9 EagI C'GGCC_G Cuts at: 1537 1812 2477 3265 1537 Size: 275 665 788 4090 Fragments arranged by size: 4090 788 665 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 3748 1353 Size: 1181 1214 3423 Fragments arranged by size: 3423 1214 1181 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 4405 4779 4925 387 Size: 1064 138 546 2270 374 146 1280 Fragments arranged by size: 2270 1280 1064 546 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3881 4478 5265 5533 1239 Size: 393 2249 597 787 268 1524 Fragments arranged by size: 2249 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2910 3101 2910 Size: 191 5627 EcoO109I rG'GnC_Cy Cuts at: 2101 2893 3059 2101 Size: 792 166 4860 Fragments arranged by size: 4860 792 166 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 2132 2746 2921 4965 1678 Size: 454 614 175 2044 2531 Fragments arranged by size: 2531 2044 614 454 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3098 3130 4459 4491 4533 Size: 32 135 32 1526 32 1329 32 42 Cuts at: 4533 4565 1373 Size: 32 2626 Fragments arranged by size: 2626 1526 1329 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 3016 748 Size: 2268 3550 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KasI G'GCGC_C Cuts at: 2742 2917 2742 Size: 175 5643 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4932 5116 1305 Size: 3627 184 2007 Fragments arranged by size: 3627 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2241 2399 2804 2945 3101 Size: 54 1425 343 409 158 405 141 156 Cuts at: 3101 10 Size: 2727 Fragments arranged by size: 2727 1425 409 405 343 158 156 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2457 3776 4135 4294 518 Size: 648 843 448 1319 359 159 2042 Fragments arranged by size: 2042 1319 843 648 448 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2107 2293 2565 2798 2878 2958 3234 Size: 424 77 186 272 233 80 80 276 Cuts at: 3234 3911 5059 5304 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 424 276 272 245 233 186 80 80 77 NaeI GCC'GGC Cuts at: 2204 2204 Size: 5818 NarI GG'CG_CC Cuts at: 2743 2918 2743 Size: 175 5643 NcoI C'CATG_G Cuts at: 513 1065 2452 513 Size: 552 1387 3879 Fragments arranged by size: 3879 1387 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NgoMIV G'CCGG_C Cuts at: 2202 2202 Size: 5818 NheI G'CTAG_C Cuts at: 1052 2110 1052 Size: 1058 4760 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NsiI A_TGCA'T Cuts at: 2983 2983 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 2374 3176 4721 1132 Size: 97 1145 802 1545 2229 Fragments arranged by size: 2229 1545 1145 802 97 PasI CC'CwG_GG Cuts at: 1348 2601 2659 1348 Size: 1253 58 4507 Fragments arranged by size: 4507 1253 58 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2970 1108 Size: 1862 3956 PfoI T'CCnGG_A Cuts at: 2691 2691 Size: 5818 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PmlI CAC'GTG Cuts at: 2788 2788 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2893 3059 2101 Size: 792 166 4860 Fragments arranged by size: 4860 792 166 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PstI C_TGCA'G Cuts at: 830 2361 3170 830 Size: 1531 809 3478 Fragments arranged by size: 3478 1531 809 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 PvuII CAG'CTG Cuts at: 2293 2798 2878 2958 2293 Size: 505 80 80 5153 Fragments arranged by size: 5153 505 80 80 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SanDI GG'GwC_CC Cuts at: 2101 2893 3059 2101 Size: 792 166 4860 Fragments arranged by size: 4860 792 166 SapI GCTCTTCn'nnn_ Cuts at: 2534 2534 Size: 5818 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 2357 3166 4301 4982 5173 826 Size: 219 1312 809 1135 681 191 1471 Fragments arranged by size: 1471 1312 1135 809 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2208 2261 2303 2208 Size: 53 42 5723 Fragments arranged by size: 5723 53 42 SfoI GGC'GCC Cuts at: 2744 2919 2744 Size: 175 5643 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 2099 2117 3035 3145 1872 Size: 227 18 918 110 4545 Fragments arranged by size: 4545 918 227 110 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 3922 4823 5085 820 Size: 197 926 1979 901 262 1553 Fragments arranged by size: 1979 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SrfI GCCC'GGGC Cuts at: 2117 2117 Size: 5818 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 2734 1517 Size: 1217 4601 StyI C'CwwG_G Cuts at: 513 1065 2452 3102 3257 513 Size: 552 1387 650 155 3074 Fragments arranged by size: 3074 1387 650 552 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2236 3064 3845 3862 4015 4200 4645 Size: 1255 656 828 781 17 153 185 445 Cuts at: 4645 325 Size: 1498 Fragments arranged by size: 1498 1255 828 781 656 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4064 5776 96 Size: 274 80 33 51 1443 2087 1712 138 Fragments arranged by size: 2087 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2772 2804 2850 2882 5778 5810 2772 Size: 32 46 32 2896 32 2780 Fragments arranged by size: 2896 2780 46 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2146 3094 2146 Size: 948 4870 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5818 XmaI C'CCGG_G Cuts at: 1870 2097 2115 3033 3143 1870 Size: 227 18 918 110 4545 Fragments arranged by size: 4545 918 227 110 18 XmnI GAAnn'nnTTC Cuts at: 3947 3947 Size: 5818 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SexAI SwaI