(Linear) (Six-base) MAPSORT of: KIBB6553.seq Check: 6931 from: 1 to: 1113 KIBB6553.orf With 182 enzymes: SgfI * October 7, 2009 14:21 .. AflIII A'CryG_T Cuts at: 0 222 782 1113 Size: 222 560 331 Fragments arranged by size: 560 331 222 AlwNI CAG_nnn'CTG Cuts at: 0 793 1113 Size: 793 320 AvaI C'yCGr_G Cuts at: 0 92 110 1028 1113 Size: 92 18 918 85 Fragments arranged by size: 918 92 85 18 BanI G'GyrC_C Cuts at: 0 213 645 708 737 852 897 912 1113 Size: 213 432 63 29 115 45 15 201 Fragments arranged by size: 432 213 201 115 63 45 29 15 BanII G_rGCy'C Cuts at: 0 462 596 780 1113 Size: 462 134 184 333 Fragments arranged by size: 462 333 184 134 BbeI G_GCGC'C Cuts at: 0 741 916 1113 Size: 741 175 197 Fragments arranged by size: 741 197 175 BbsI GAAGACnn'nnnn_ Cuts at: 0 219 1113 Size: 219 894 BfrBI ATG'CAT Cuts at: 0 976 1113 Size: 976 137 BglI GCCn_nnn'nGGC Cuts at: 0 203 256 298 735 758 1113 Size: 203 53 42 437 23 355 Fragments arranged by size: 437 355 203 53 42 23 Bme1580I G_kGCm'C Cuts at: 0 216 855 1029 1113 Size: 216 639 174 84 Fragments arranged by size: 639 216 174 84 BmgBI CAC'GTC Cuts at: 0 225 1113 Size: 225 888 BmrI ACTGGGnnnn_n' Cuts at: 0 219 1113 Size: 219 894 BmtI G_CTAG'C Cuts at: 0 109 1113 Size: 109 1004 Bpu10I CC'TnA_GC Cuts at: 0 157 1113 Size: 157 956 BsaAI yAC'GTr Cuts at: 0 783 1113 Size: 783 330 BsaHI Gr'CG_yC Cuts at: 0 10 738 750 913 1113 Size: 10 728 12 163 200 Fragments arranged by size: 728 200 163 12 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 767 797 1113 Size: 767 30 316 Fragments arranged by size: 767 316 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 366 1077 1113 Size: 366 711 36 Fragments arranged by size: 711 366 36 BseYI C'CCAG_C Cuts at: 0 49 58 284 518 536 575 848 1045 Size: 49 9 226 234 18 39 273 197 Cuts at: 1045 1113 Size: 68 Fragments arranged by size: 273 234 226 197 68 49 39 18 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 889 939 1056 1113 Size: 889 50 117 57 Fragments arranged by size: 889 117 57 50 BsiEI CG_ry'CG Cuts at: 0 309 475 1113 Size: 309 166 638 Fragments arranged by size: 638 309 166 BsiHKAI G_wGCw'C Cuts at: 0 680 1113 Size: 680 433 Bsp1286I G_dGCh'C Cuts at: 0 216 462 596 680 780 855 1029 1113 Size: 216 246 134 84 100 75 174 84 Fragments arranged by size: 246 216 174 134 100 84 84 75 BsrDI GCAATG_nn' Cuts at: 0 168 1113 Size: 168 945 BsrFI r'CCGG_y Cuts at: 0 197 470 474 1113 Size: 197 273 4 639 Fragments arranged by size: 639 273 197 4 BstXI CCAn_nnnn'nTGG Cuts at: 0 402 454 1113 Size: 402 52 659 Fragments arranged by size: 659 402 52 BstYI r'GATC_y Cuts at: 0 968 1113 Size: 968 145 BtgI C'CryG_G Cuts at: 0 260 315 447 1113 Size: 260 55 132 666 Fragments arranged by size: 666 260 132 55 EaeI y'GGCC_r Cuts at: 0 234 248 257 392 472 797 938 1094 Size: 234 14 9 135 80 325 141 156 Cuts at: 1094 1113 Size: 19 Fragments arranged by size: 325 234 156 141 135 80 19 14 9 EagI C'GGCC_G Cuts at: 0 472 1113 Size: 472 641 EarI CTCTTCn'nnn_ Cuts at: 0 529 1113 Size: 529 584 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 130 1113 Size: 130 983 EcoNI CCTnn'n_nnAGG Cuts at: 0 905 1096 1113 Size: 905 191 17 Fragments arranged by size: 905 191 17 EcoO109I rG'GnC_Cy Cuts at: 0 96 888 1054 1113 Size: 96 792 166 59 Fragments arranged by size: 792 166 96 59 HaeII r_GCGC'y Cuts at: 0 127 741 916 1113 Size: 127 614 175 197 Fragments arranged by size: 614 197 175 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1093 1113 Size: 1093 20 HindIII A'AGCT_T Cuts at: 0 1011 1113 Size: 1011 102 KasI G'GCGC_C Cuts at: 0 737 912 1113 Size: 737 175 201 Fragments arranged by size: 737 201 175 MscI TGG'CCA Cuts at: 0 236 394 799 940 1096 1113 Size: 236 158 405 141 156 17 Fragments arranged by size: 405 236 158 156 141 17 MslI CAynn'nnrTG Cuts at: 0 452 1113 Size: 452 661 MspA1I CmG'CkG Cuts at: 0 25 102 288 560 793 873 953 1113 Size: 25 77 186 272 233 80 80 160 Fragments arranged by size: 272 233 186 160 80 80 77 25 NaeI GCC'GGC Cuts at: 0 199 1113 Size: 199 914 NarI GG'CG_CC Cuts at: 0 738 913 1113 Size: 738 175 200 Fragments arranged by size: 738 200 175 NcoI C'CATG_G Cuts at: 0 447 1113 Size: 447 666 NgoMIV G'CCGG_C Cuts at: 0 197 1113 Size: 197 916 NheI G'CTAG_C Cuts at: 0 105 1113 Size: 105 1008 NsiI A_TGCA'T Cuts at: 0 978 1113 Size: 978 135 NspI r_CATG'y Cuts at: 0 369 1113 Size: 369 744 PasI CC'CwG_GG Cuts at: 0 596 654 1113 Size: 596 58 459 Fragments arranged by size: 596 459 58 PflMI CCAn_nnn'nTGG Cuts at: 0 965 1113 Size: 965 148 PfoI T'CCnGG_A Cuts at: 0 686 1113 Size: 686 427 PmlI CAC'GTG Cuts at: 0 783 1113 Size: 783 330 PpuMI rG'GwC_Cy Cuts at: 0 96 888 1054 1113 Size: 96 792 166 59 Fragments arranged by size: 792 166 96 59 PstI C_TGCA'G Cuts at: 0 356 1113 Size: 356 757 PvuII CAG'CTG Cuts at: 0 288 793 873 953 1113 Size: 288 505 80 80 160 Fragments arranged by size: 505 288 160 80 80 SanDI GG'GwC_CC Cuts at: 0 96 888 1054 1113 Size: 96 792 166 59 Fragments arranged by size: 792 166 96 59 SapI GCTCTTCn'nnn_ Cuts at: 0 529 1113 Size: 529 584 SfcI C'TryA_G Cuts at: 0 352 1113 Size: 352 761 SfiI GGCCn_nnn'nGGCC Cuts at: 0 203 256 298 1113 Size: 203 53 42 815 Fragments arranged by size: 815 203 53 42 SfoI GGC'GCC Cuts at: 0 739 914 1113 Size: 739 175 199 Fragments arranged by size: 739 199 175 SmaI CCC'GGG Cuts at: 0 94 112 1030 1113 Size: 94 18 918 83 Fragments arranged by size: 918 94 83 18 SrfI GCCC'GGGC Cuts at: 0 112 1113 Size: 112 1001 StuI AGG'CCT Cuts at: 0 729 1113 Size: 729 384 StyI C'CwwG_G Cuts at: 0 447 1097 1113 Size: 447 650 16 Fragments arranged by size: 650 447 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 231 1059 1113 Size: 231 828 54 Fragments arranged by size: 828 231 54 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 767 799 845 877 1113 Size: 767 32 46 32 236 Fragments arranged by size: 767 236 46 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 141 1089 1113 Size: 141 948 24 Fragments arranged by size: 948 141 24 XmaI C'CCGG_G Cuts at: 0 92 110 1028 1113 Size: 92 18 918 85 Fragments arranged by size: 918 92 85 18 Enzymes that do cut: AflIII AlwNI AvaI BanI BanII BbeI BbsI BfrBI BglI Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BstXI BstYI BtgI EaeI EagI EarI EciI EcoNI EcoO109I HaeII Hin4I HindIII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PflMI PfoI PmlI PpuMI PstI PvuII SanDI SapI SfcI SfiI SfoI SmaI SrfI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BglII BlpI BplI BpmI BpuEI BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI NdeI NotI NruI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI