Restriction map for
pFN21AB6548
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbsI
BcgI BciVI BclI BglII BlnI
BpuAI BsaWI BseAI BsgI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KspI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 406 GGTACC G^GTACC
AcyI 898 GACGCC G[GA]^CG[CT]C
AflII 982 CTTAAG C^TTAAG
Asp718I 406 GGTACC G^GTACC
AspHI 16 GTGCTC G[AT]GC[AT]^C
BanI 406 GGTACC G^G[CT][GA]CC
BbrPI 112 CACGTG CAC^GTG
BfaI 394 CTAG C^TAG
BfrI 982 CTTAAG C^TTAAG
BpmI 27 CTCCAG CTGGAG(16/14)
BsaAI 112 CACGTG [CT]AC^GT[GA]
BsaBI 1113 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 898 GACGCC G[GA]^CG[CT]C
BsaI 198 GGTCTC GGTCTC(1/5)
BsiEI 851 CGGCCG CG[GA][CT]^CG
BsiHKAI 16 GTGCTC G[AT]GC[AT]^C
BsmI 42 GAATGC GAATGC(1/-1)
BstEII 135 GGTGACC G^GT[ACGT]ACC
BstPI 135 GGTGACC G^GT[ACGT]ACC
BstXI 821 CCATCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 851 CGGCCG C^GGCCG
EclXI 851 CGGCCG C^GGCCG
Eco52I 851 CGGCCG C^GGCCG
EcoO65I 135 GGTGACC G^GT[ACGT]ACC
HaeII 597 GGCGCT [GA]GCGC^[CT]
HgaI 898 GACGC GACGC(5/10)
Hin1I 898 GACGCC G[GA]^CG[CT]C
KpnI 406 GGTACC GGTAC^C
MaeI 394 CTAG C^TAG
MamI 1113 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 983 TTAA T^TAA
MspA1I 1031 CAGCTG C[AC]G^C[GT]G
NaeI 849 GCCGGC GCC^GGC
NgoMIV 849 GCCGGC G^CCGGC
PmaCI 112 CACGTG CAC^GTG
PmlI 112 CACGTG CAC^GTG
PstI 425 CTGCAG CTGCA^G
PvuII 1031 CAGCTG CAG^CTG
SfcI 425 CTGCAG C^T[GA][CT]AG
SmaI 729 CCCGGG CCC^GGG
SmlI 982 CTTAAG C^T[CT][GA]AG
Tru9I 983 TTAA T^TAA
XmaCI 729 CCCGGG C^CCGGG
XmaI 729 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 111 ACACGT A^C[GA][CT]GT
767 ACGTGT
ApaI 871 GGGCCC GGGCC^C
1050 GGGCCC
AvaI 175 CCCGAG C^[CT]CG[GA]G
729 CCCGGG
BbvCI 885 CCTCAGC CCTCAGC(-5/-2)
571 GCTGAGG
BglI 367 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
554 GCCTGAAGGGC
BlpI 958 GCTGAGC GC^T[ACGT]AGC
1006 GCTAAGC
Bpu1102I 958 GCTGAGC GC^T[ACGT]AGC
1006 GCTAAGC
BsmFI 896 GGGAC GGGAC(10/14)
6 GTCCC
BsoBI 175 CCCGAG C^[CT]CG[GA]G
729 CCCGGG
BsrFI 849 GCCGGC [GA]^CCGG[CT]
1011 GCCGGT
CelII 958 GCTGAGC GC^T[ACGT]AGC
1006 GCTAAGC
Cfr10I 849 GCCGGC [GA]^CCGG[CT]
1011 GCCGGT
DsaI 776 CCATGG C^C[GA][CT]GG
1103 CCATGG
FauI 815 GCGGG CCCGC(4/6)
1048 GCGGG
MaeII 113 ACGT A^CGT
767 ACGT
NcoI 776 CCATGG C^CATGG
1103 CCATGG
NlaIII 777 CATG CATG^
1104 CATG
PleI 835 GACTC GAGTC(4/5)
304 GAGTC
PpuMI 287 AGGTCCT [GA]G^G[AT]CC[CT]
752 AGGACCC
SfaNI 364 GATGC GCATC(5/9)
568 GATGC
TaqI 59 TCGA T^CGA
500 TCGA
TspRI 51 CACTG CA[GC]TG[ACGT][ACGT]^
998 CAGTG
TthHB8I 59 TCGA T^CGA
500 TCGA
XcmI 989 CCAAGCCACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1103 CCATGGACGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIV PflMI
RsaI Sau3AI Sau96I ScrFI StyI
TfiI TseI Tsp45I Van91I XhoII