(Circular) (Six-base) MAPSORT of: pFN21AB6548.seq Check: 5191 from: 1 to: 5881 pFN21AB6548 5881 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 2411 3202 2411 Size: 791 5090 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2027 2582 2813 3084 3944 5328 5596 1632 Size: 395 555 231 271 860 1384 268 1917 Fragments arranged by size: 1917 1384 860 555 395 271 268 231 AflII C'TTAA_G Cuts at: 820 1017 2987 820 Size: 197 1970 3714 Fragments arranged by size: 3714 1970 197 AflIII A'CryG_T Cuts at: 1203 2116 2772 4780 5757 1203 Size: 913 656 2008 977 1327 Fragments arranged by size: 2008 1327 977 913 656 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2134 2245 2429 2560 2950 3624 5196 2134 Size: 111 184 131 390 674 1572 2819 Fragments arranged by size: 2819 1572 674 390 184 131 111 ApaI G_GGCC'C Cuts at: 2880 3059 2880 Size: 179 5702 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 3190 3416 3568 5677 1493 Size: 1697 226 152 2109 1697 Fragments arranged by size: 2109 1697 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2180 2734 3206 1870 Size: 73 237 554 472 4545 Fragments arranged by size: 4545 554 472 237 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2411 3202 4657 618 Size: 325 217 627 624 791 1455 1842 Fragments arranged by size: 1842 1455 791 627 624 325 217 BanII G_rGCy'C Cuts at: 1391 2439 2727 2880 2970 3059 3200 1391 Size: 1048 288 153 90 89 141 4072 Fragments arranged by size: 4072 1048 288 153 141 90 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BbvCI CC'TCA_GC Cuts at: 2577 2891 2577 Size: 314 5567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4072 4106 1903 Size: 34 50 34 2051 34 3678 Fragments arranged by size: 3678 2051 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2378 2565 4492 Size: 107 122 71 631 605 706 187 1927 Cuts at: 4492 136 Size: 1525 Fragments arranged by size: 1927 1525 706 631 605 187 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 2964 3012 3298 2964 Size: 48 286 5547 Fragments arranged by size: 5547 286 48 Bme1580I G_kGCm'C Cuts at: 1790 2079 2880 3059 3942 5098 1790 Size: 289 801 179 883 1156 2573 Fragments arranged by size: 2573 1156 883 801 289 179 BmgBI CAC'GTC Cuts at: 1318 2773 1318 Size: 1455 4426 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3612 4570 442 Size: 416 53 83 399 24 2195 958 1753 Fragments arranged by size: 2195 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2017 4541 1239 Size: 778 2524 2579 Fragments arranged by size: 2579 2524 778 Bpu10I CC'TnA_GC Cuts at: 2577 2891 2577 Size: 314 5567 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4006 4871 5169 4006 Size: 865 298 4718 Fragments arranged by size: 4718 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2209 4544 882 Size: 617 710 2335 2219 Fragments arranged by size: 2335 2219 710 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 3672 493 Size: 711 915 1553 2702 Fragments arranged by size: 2702 1553 915 711 BsaBI GATnn'nnATC Cuts at: 3122 5875 3122 Size: 2753 3128 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2904 3688 4070 4705 Size: 53 83 186 936 1371 784 382 635 Cuts at: 4705 275 Size: 1451 Fragments arranged by size: 1451 1371 936 784 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2759 2789 1178 Size: 30 165 30 1356 30 4270 Fragments arranged by size: 4270 1356 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2341 2531 2606 2609 2612 2717 1186 Size: 1057 98 190 75 3 3 105 4350 Fragments arranged by size: 4350 1057 190 105 98 75 3 3 BseYI C'CCAG_C Cuts at: 2079 2885 2982 3074 3170 5084 5580 2079 Size: 806 97 92 96 1914 496 2380 Fragments arranged by size: 2380 1914 806 496 97 96 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2859 3331 4092 5120 1540 Size: 275 186 858 472 761 1028 2301 Fragments arranged by size: 2301 1028 858 761 472 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 3942 4027 5098 2025 Size: 1917 85 1071 2808 Fragments arranged by size: 2808 1917 1071 85 BsmI GAATG_Cn' Cuts at: 2053 3392 3485 2053 Size: 1339 93 4449 Fragments arranged by size: 4449 1339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2079 2439 2727 2880 2970 3059 Size: 399 235 54 360 288 153 90 89 Cuts at: 3059 3200 3942 4027 5098 1391 Size: 141 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 288 235 153 141 90 89 85 54 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3236 844 Size: 2392 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 4550 65 Size: 1183 3128 174 1396 Fragments arranged by size: 3128 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2854 3016 4525 4712 1700 Size: 110 1044 162 1509 187 2869 Fragments arranged by size: 2869 1509 1044 187 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5881 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 2140 1184 Size: 956 4925 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2833 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2130 2346 2417 3099 3211 3578 3963 3980 Size: 158 216 71 682 112 367 385 17 Cuts at: 3980 5432 5518 5530 5876 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 682 385 367 346 216 158 112 86 71 17 12 BtgI C'CryG_G Cuts at: 513 1065 2781 3108 513 Size: 552 1716 327 3286 Fragments arranged by size: 3286 1716 552 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 3397 4191 4211 3397 Size: 794 20 5067 Fragments arranged by size: 5067 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2658 2856 Size: 54 1425 50 275 18 336 492 198 Cuts at: 2856 3328 4217 5846 8 Size: 472 889 1629 43 Fragments arranged by size: 1629 1425 889 492 472 336 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2856 3328 1537 Size: 275 1044 472 4090 Fragments arranged by size: 4090 1044 472 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2490 2532 2925 3182 3811 1353 Size: 1137 42 393 257 629 3423 Fragments arranged by size: 3423 1137 629 393 257 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2535 4468 4842 4988 387 Size: 1064 138 946 1933 374 146 1280 Fragments arranged by size: 1933 1280 1064 946 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2017 2027 2582 2813 3084 3944 4541 Size: 393 385 10 555 231 271 860 597 Cuts at: 4541 5328 5596 1239 Size: 787 268 1524 Fragments arranged by size: 1524 860 787 597 555 393 385 271 268 231 10 EcoO109I rG'GnC_Cy Cuts at: 2293 2758 2876 2293 Size: 465 118 5298 Fragments arranged by size: 5298 465 118 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 2606 5028 1678 Size: 928 2422 2531 Fragments arranged by size: 2531 2422 928 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2762 2791 2794 4522 Size: 32 135 32 1187 3 29 3 1728 Cuts at: 4522 4554 4596 4628 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1728 1187 135 42 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 2415 3206 2415 Size: 791 5090 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4995 5179 1305 Size: 3690 184 2007 Fragments arranged by size: 3690 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3839 4198 4357 518 Size: 648 2673 359 159 2042 Fragments arranged by size: 2673 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3038 3297 3974 5122 5367 1606 Size: 1432 259 677 1148 245 2120 Fragments arranged by size: 2120 1432 1148 677 259 245 NaeI GCC'GGC Cuts at: 2856 2856 Size: 5881 NcoI C'CATG_G Cuts at: 513 1065 2781 3108 513 Size: 552 1716 327 3286 Fragments arranged by size: 3286 1716 552 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NgoMIV G'CCGG_C Cuts at: 2854 2854 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 3239 4784 1132 Size: 97 2010 1545 2229 Fragments arranged by size: 2229 2010 1545 97 PasI CC'CwG_GG Cuts at: 1348 2423 2833 1348 Size: 1075 410 4396 Fragments arranged by size: 4396 1075 410 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 2200 3109 1108 Size: 1026 66 909 3880 Fragments arranged by size: 3880 1026 909 66 PfoI T'CCnGG_A Cuts at: 2319 2460 3138 2319 Size: 141 678 5062 Fragments arranged by size: 5062 678 141 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PmlI CAC'GTG Cuts at: 2119 2119 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2758 2293 Size: 465 5416 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PspOMI G'GGCC_C Cuts at: 2876 3055 2876 Size: 179 5702 PstI C_TGCA'G Cuts at: 830 2434 3233 830 Size: 1604 799 3478 Fragments arranged by size: 3478 1604 799 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 3038 3038 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2430 3229 4364 5045 5236 826 Size: 219 1385 799 1135 681 191 1471 Fragments arranged by size: 1471 1385 1135 799 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 2736 3208 1872 Size: 864 472 4545 Fragments arranged by size: 4545 864 472 SmlI C'TyrA_G Cuts at: 820 1017 1943 2987 3985 4886 5148 820 Size: 197 926 1044 998 901 262 1553 Fragments arranged by size: 1553 1044 998 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SrfI GCCC'GGGC Cuts at: 2736 2736 Size: 5881 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2364 2586 2781 2910 3108 3320 513 Size: 552 1299 222 195 129 198 212 3074 Fragments arranged by size: 3074 1299 552 222 212 198 195 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2825 2899 3908 3925 4078 4263 4708 Size: 1255 1245 74 1009 17 153 185 445 Cuts at: 4708 325 Size: 1498 Fragments arranged by size: 1498 1255 1245 1009 445 185 153 74 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 3132 4127 Size: 274 80 33 51 1443 237 918 995 Cuts at: 4127 5839 96 Size: 1712 138 Fragments arranged by size: 1712 1443 995 918 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5841 5873 5841 Size: 32 5849 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3001 3115 3001 Size: 114 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 2734 3206 1870 Size: 864 472 4545 Fragments arranged by size: 4545 864 472 XmnI GAAnn'nnTTC Cuts at: 4010 4010 Size: 5881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI