(Circular) (Six-base) MAPSORT of: pFN21AB6537.seq Check: 1208 from: 1 to: 5806 pFN21AB6537 5806 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3869 5253 5521 1632 Size: 2237 1384 268 1917 Fragments arranged by size: 2237 1917 1384 268 AfeI AGC'GCT Cuts at: 2111 2399 2987 2111 Size: 288 588 4930 Fragments arranged by size: 4930 588 288 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2130 3087 1184 Size: 946 957 3903 Fragments arranged by size: 3903 957 946 AlwNI CAG_nnn'CTG Cuts at: 3549 5121 3549 Size: 1572 4234 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 2789 2855 3115 3341 3493 5602 1493 Size: 1296 66 260 226 152 2109 1697 Fragments arranged by size: 2109 1697 1296 260 226 152 66 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2283 2468 2850 3131 1870 Size: 73 340 185 382 281 4545 Fragments arranged by size: 4545 382 340 281 185 73 BamHI G'GATC_C Cuts at: 3136 3503 3136 Size: 367 5439 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 3127 4582 618 Size: 325 217 627 269 1071 1455 1842 Fragments arranged by size: 1842 1455 1071 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 2290 2355 2694 2963 3125 1391 Size: 899 65 339 269 162 4072 Fragments arranged by size: 4072 899 339 269 162 65 BbeI G_GCGC'C Cuts at: 2060 2060 Size: 5806 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3108 928 Size: 971 1209 3626 Fragments arranged by size: 3626 1209 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 1548 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4417 136 Size: 107 122 71 631 605 2745 1525 Fragments arranged by size: 2745 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 3223 3223 Size: 5806 Bme1580I G_kGCm'C Cuts at: 1790 3867 5023 1790 Size: 2077 1156 2573 Fragments arranged by size: 2573 2077 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3043 4466 1239 Size: 1804 1423 2579 Fragments arranged by size: 2579 1804 1423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3931 4796 5094 3931 Size: 865 298 4643 Fragments arranged by size: 4643 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4469 882 Size: 617 2970 2219 Fragments arranged by size: 2970 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2322 2428 3597 493 Size: 711 1118 106 1169 2702 Fragments arranged by size: 2702 1169 1118 711 106 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 3613 3995 4630 Size: 53 83 186 936 524 1556 382 635 Cuts at: 4630 275 Size: 1451 Fragments arranged by size: 1556 1451 936 635 524 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 2887 4239 4637 4911 5058 5469 2487 Size: 400 1352 398 274 147 411 2824 Fragments arranged by size: 2824 1352 411 400 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 2831 2861 1178 Size: 30 165 30 1362 30 36 30 4123 Fragments arranged by size: 4123 1362 165 36 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 2855 1186 Size: 873 796 4137 Fragments arranged by size: 4137 873 796 BseYI C'CCAG_C Cuts at: 2963 3046 5009 5505 2963 Size: 83 1963 496 3264 Fragments arranged by size: 3264 1963 496 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 2136 1163 Size: 573 390 10 4833 Fragments arranged by size: 4833 573 390 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3256 4017 5045 1540 Size: 275 186 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2290 2355 2694 2899 3867 3952 5023 2290 Size: 65 339 205 968 85 1071 3073 Fragments arranged by size: 3073 1071 968 339 205 85 65 BsmI GAATG_Cn' Cuts at: 2452 3317 3410 2452 Size: 865 93 4848 Fragments arranged by size: 4848 865 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2290 2355 2694 2899 2963 3125 3867 Size: 399 500 65 339 205 64 162 742 Cuts at: 3867 3952 5023 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 399 339 205 162 85 65 64 BspEI T'CCGG_A Cuts at: 2887 5469 2887 Size: 2582 3224 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2673 3161 844 Size: 1829 488 3489 Fragments arranged by size: 3489 1829 488 BsrBI CCG'CTC Cuts at: 1394 1823 3693 1394 Size: 429 1870 3507 Fragments arranged by size: 3507 1870 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4301 4475 65 Size: 1183 3053 174 1396 Fragments arranged by size: 3053 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4450 4637 1700 Size: 110 2640 187 2869 Fragments arranged by size: 2869 2640 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5806 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstYI r'GATC_y Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801 Size: 1164 367 385 17 1452 86 12 346 Cuts at: 5801 1972 Size: 1977 Fragments arranged by size: 1977 1452 1164 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4315 Fragments arranged by size: 4315 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2350 2416 2542 3322 4116 4136 2124 Size: 226 66 126 780 794 20 3794 Fragments arranged by size: 3794 794 780 226 126 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2478 3253 4142 Size: 54 1425 50 275 18 648 775 889 Cuts at: 4142 5771 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 775 648 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3253 1537 Size: 275 1441 4090 Fragments arranged by size: 4090 1441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 3736 1353 Size: 683 1700 3423 Fragments arranged by size: 3423 1700 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2674 4393 4767 4913 387 Size: 1064 138 1085 1719 374 146 1280 Fragments arranged by size: 1719 1280 1085 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2288 2353 2692 2288 Size: 65 339 5402 Fragments arranged by size: 5402 339 65 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3043 3869 4466 5253 5521 1239 Size: 393 1411 826 597 787 268 1524 Fragments arranged by size: 1524 1411 826 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2182 2182 Size: 5806 EcoRI G'AATT_C Cuts at: 2855 3115 3493 2855 Size: 260 378 5168 Fragments arranged by size: 5168 378 260 EcoRV GAT'ATC Cuts at: 3085 3085 Size: 5806 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2060 2113 2401 2989 4953 1678 Size: 382 53 288 588 1964 2531 Fragments arranged by size: 2531 1964 588 382 288 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2765 2797 2831 2863 4447 Size: 32 135 32 1193 32 34 32 1584 Cuts at: 4447 4479 4521 4553 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1584 1193 135 42 34 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2164 2743 3150 3396 669 Size: 988 281 226 579 407 246 3079 Fragments arranged by size: 3079 988 579 407 281 246 226 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KasI G'GCGC_C Cuts at: 2056 2056 Size: 5806 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2606 4920 5104 1305 Size: 1301 2314 184 2007 Fragments arranged by size: 2314 2007 1301 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 3764 4123 4282 518 Size: 648 1215 1383 359 159 2042 Fragments arranged by size: 2042 1383 1215 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3222 3899 5047 5292 1606 Size: 1616 677 1148 245 2120 Fragments arranged by size: 2120 1616 1148 677 245 NarI GG'CG_CC Cuts at: 2057 2057 Size: 5806 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4315 Fragments arranged by size: 4315 939 552 NdeI CA'TA_TG Cuts at: 387 2907 387 Size: 2520 3286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 3253 3253 Size: 5806 NruI TCG'CGA Cuts at: 2982 2982 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 2575 3164 4709 1132 Size: 97 1346 589 1545 2229 Fragments arranged by size: 2229 1545 1346 589 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5806 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 2550 3376 2550 Size: 826 4980 PstI C_TGCA'G Cuts at: 830 2670 2940 3076 3158 830 Size: 1840 270 136 82 3478 Fragments arranged by size: 3478 1840 270 136 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 SacI G_AGCT'C Cuts at: 2290 2355 2694 2290 Size: 65 339 5402 Fragments arranged by size: 5402 339 65 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2666 2936 3072 3154 4289 4970 5161 Size: 219 1621 270 136 82 1135 681 191 Cuts at: 5161 826 Size: 1471 Fragments arranged by size: 1621 1471 1135 681 270 219 191 136 82 SfoI GGC'GCC Cuts at: 2058 2058 Size: 5806 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 3133 1872 Size: 1261 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3910 4811 5073 820 Size: 197 926 1967 901 262 1553 Fragments arranged by size: 1967 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2322 3597 493 Size: 1829 1275 2702 Fragments arranged by size: 2702 1829 1275 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 3164 1132 Size: 2032 3774 SspI AAT'ATT Cuts at: 5 52 2256 3730 5 Size: 47 2204 1474 2081 Fragments arranged by size: 2204 2081 1474 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 2004 2025 3245 513 Size: 552 939 21 1220 3074 Fragments arranged by size: 3074 1220 939 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3833 3850 4003 4188 4633 325 Size: 1255 2253 17 153 185 445 1498 Fragments arranged by size: 2253 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2457 2489 5766 5798 2000 Size: 32 425 32 3277 32 2008 Fragments arranged by size: 3277 2008 425 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2408 1316 Size: 1092 4714 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 3131 1870 Size: 1261 4545 XmnI GAAnn'nnTTC Cuts at: 2309 2793 3935 2309 Size: 484 1142 4180 Fragments arranged by size: 4180 1142 484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I FalI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI