(Circular) (Six-base) MAPSORT of: pFN21AB6695.seq Check: 7747 from: 1 to: 5992 pFN21AB6695 5992 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3112 3112 Size: 5992 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 3015 4055 5439 5707 1632 Size: 734 649 1040 1384 268 1917 Fragments arranged by size: 1917 1384 1040 734 649 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 4891 5868 1203 Size: 3688 977 1327 Fragments arranged by size: 3688 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 2131 2647 4721 2131 Size: 516 2074 3402 Fragments arranged by size: 3402 2074 516 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2463 2837 1184 Size: 1279 374 4339 Fragments arranged by size: 4339 1279 374 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 5960 AlwNI CAG_nnn'CTG Cuts at: 2182 3735 5307 2182 Size: 1553 1572 2867 Fragments arranged by size: 2867 1572 1553 ApaI G_GGCC'C Cuts at: 2282 3175 2282 Size: 893 5099 ApaLI G'TGCA_C Cuts at: 2415 4049 5205 2415 Size: 1634 1156 3202 Fragments arranged by size: 3202 1634 1156 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 BamHI G'GATC_C Cuts at: 3232 3322 3689 3232 Size: 90 367 5535 Fragments arranged by size: 5535 367 90 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3124 3313 4768 618 Size: 325 217 627 1337 189 1455 1842 Fragments arranged by size: 1842 1455 1337 627 325 217 189 BanII G_rGCy'C Cuts at: 1391 2149 2282 2594 3069 3175 3311 1391 Size: 758 133 312 475 106 136 4072 Fragments arranged by size: 4072 758 475 312 136 133 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2403 928 Size: 971 504 4517 Fragments arranged by size: 4517 971 504 BbvCI CC'TCA_GC Cuts at: 2433 2433 Size: 5992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 2730 1548 Size: 1182 4810 BfrBI ATG'CAT Cuts at: 2814 2814 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3073 4603 136 Size: 107 122 71 631 605 1401 1530 1525 Fragments arranged by size: 1530 1525 1401 631 605 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 2282 2419 2480 3129 3175 4053 5209 1790 Size: 492 137 61 649 46 878 1156 2573 Fragments arranged by size: 2573 1156 878 649 492 137 61 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3723 4681 442 Size: 416 53 83 399 24 2306 958 1753 Fragments arranged by size: 2306 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2560 2560 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2240 2277 4652 1239 Size: 842 159 37 2375 2579 Fragments arranged by size: 2579 2375 842 159 37 Bpu10I CC'TnA_GC Cuts at: 2433 3017 2433 Size: 584 5408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 3224 4117 4982 5280 2363 Size: 861 893 865 298 3075 Fragments arranged by size: 3075 893 865 861 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3176 4655 882 Size: 617 1677 1479 2219 Fragments arranged by size: 2219 1677 1479 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3799 4181 4816 275 Size: 53 83 186 936 2266 382 635 1451 Fragments arranged by size: 2266 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2253 2283 2409 Size: 30 165 30 597 30 223 30 126 Cuts at: 2409 2439 3273 3303 1178 Size: 30 834 30 3867 Fragments arranged by size: 3867 834 597 223 165 126 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2274 2449 2450 2501 3290 1186 Size: 1088 175 1 51 789 3888 Fragments arranged by size: 3888 1088 789 175 51 1 BseYI C'CCAG_C Cuts at: 2749 5195 5691 2749 Size: 2446 496 3050 Fragments arranged by size: 3050 2446 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2059 3116 1163 Size: 573 323 1057 4039 Fragments arranged by size: 4039 1057 573 323 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 2419 3051 4053 4138 5209 2108 Size: 311 632 1002 85 1071 2891 Fragments arranged by size: 2891 1071 1002 632 311 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2149 2282 2419 2480 2594 3051 Size: 399 318 41 133 137 61 114 457 Cuts at: 3051 3069 3129 3175 3311 4053 4138 5209 1391 Size: 18 60 46 136 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 399 318 137 136 133 114 85 61 60 46 41 18 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3112 3347 844 Size: 2268 235 3489 Fragments arranged by size: 3489 2268 235 BsrBI CCG'CTC Cuts at: 1394 1823 3879 1394 Size: 429 2056 3507 Fragments arranged by size: 3507 2056 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4487 4661 65 Size: 1183 3239 174 1396 Fragments arranged by size: 3239 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3117 4636 4823 1700 Size: 110 1307 1519 187 2869 Fragments arranged by size: 2869 1519 1307 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 4052 5064 4052 Size: 1012 4980 BstAPI GCAn_nnn'nTGC Cuts at: 794 2929 794 Size: 2135 3857 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2557 2557 Size: 5992 BstYI r'GATC_y Cuts at: 1972 2179 3232 3322 3689 4074 4091 5543 5629 Size: 207 1053 90 367 385 17 1452 86 Cuts at: 5629 5641 5987 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1053 385 367 346 207 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2370 2529 2370 Size: 159 5833 BtgI C'CryG_G Cuts at: 513 1065 2004 2556 513 Size: 552 939 552 3949 Fragments arranged by size: 3949 939 552 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 2359 2550 3508 4302 4322 2359 Size: 191 958 794 20 4029 Fragments arranged by size: 4029 958 794 191 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2150 2185 2255 2290 529 Size: 35 1586 35 70 35 4231 Fragments arranged by size: 4231 1586 70 35 35 35 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957 Size: 54 1425 50 275 18 1609 889 1629 Cuts at: 5957 8 Size: 43 Fragments arranged by size: 1629 1609 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2529 2991 3922 1353 Size: 710 466 462 931 3423 Fragments arranged by size: 3423 931 710 466 462 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2240 2277 2366 3015 4055 4652 Size: 393 449 159 37 89 649 1040 597 Cuts at: 4652 5439 5707 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1040 787 649 597 449 393 268 159 89 37 EcoNI CCTnn'n_nnAGG Cuts at: 2373 2373 Size: 5992 EcoO109I rG'GnC_Cy Cuts at: 2278 2279 3171 2278 Size: 1 892 5099 Fragments arranged by size: 5099 892 1 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 5139 1678 Size: 3461 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2576 2608 2633 Size: 32 135 32 545 32 427 32 25 Cuts at: 2633 2665 3273 3305 4633 4665 4707 4739 1373 Size: 32 608 32 1328 32 42 32 2626 Fragments arranged by size: 2626 1328 608 545 427 135 42 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2136 3336 3582 669 Size: 988 281 198 1200 246 3079 Fragments arranged by size: 3079 1200 988 281 246 198 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5106 5290 1305 Size: 3801 184 2007 Fragments arranged by size: 3801 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4170 Fragments arranged by size: 4170 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 2837 2868 3950 4309 4468 518 Size: 648 1389 282 31 1082 359 159 2042 Fragments arranged by size: 2042 1389 1082 648 359 282 159 31 MspA1I CmG'CkG Cuts at: 1606 3408 4085 5233 5478 1606 Size: 1802 677 1148 245 2120 Fragments arranged by size: 2120 1802 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2556 513 Size: 552 939 552 3949 Fragments arranged by size: 3949 939 552 552 NdeI CA'TA_TG Cuts at: 387 2152 387 Size: 1765 4227 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NsiI A_TGCA'T Cuts at: 2816 2816 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2818 2932 3147 3350 4895 1132 Size: 97 1589 114 215 203 1545 2229 Fragments arranged by size: 2229 1589 1545 215 203 114 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5992 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2000 2032 4061 4093 5574 5606 2000 Size: 32 2029 32 1481 32 2386 Fragments arranged by size: 2386 2029 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2229 1613 Size: 616 5376 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 2278 3171 2278 Size: 893 5099 PstI C_TGCA'G Cuts at: 830 3344 830 Size: 2514 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SexAI A'CCwGG_T Cuts at: 3108 3108 Size: 5992 SfcI C'TryA_G Cuts at: 826 1045 2165 3340 4475 5156 5347 826 Size: 219 1120 1175 1135 681 191 1471 Fragments arranged by size: 1471 1175 1135 1120 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 3203 4096 4997 5259 820 Size: 197 926 399 861 893 901 262 1553 Fragments arranged by size: 1553 926 901 893 861 399 262 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 2818 2932 3147 3350 1132 Size: 1686 114 215 203 3774 Fragments arranged by size: 3774 1686 215 203 114 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 2379 1517 Size: 862 5130 StyI C'CwwG_G Cuts at: 513 1065 2004 2556 3167 3431 513 Size: 552 939 552 611 264 3074 Fragments arranged by size: 3074 939 611 552 552 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 2402 2951 3082 4019 4036 4189 Size: 1255 723 99 549 131 937 17 153 Cuts at: 4189 4374 4819 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 937 723 549 445 185 153 131 99 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3207 4238 5950 Size: 274 80 33 51 1443 1230 1031 1712 Cuts at: 5950 96 Size: 138 Fragments arranged by size: 1712 1443 1230 1031 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2253 2285 2409 2441 5952 5984 2085 Size: 32 136 32 124 32 3511 32 2093 Fragments arranged by size: 3511 2093 136 124 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 2482 3328 2482 Size: 846 5146 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2166 2252 2166 Size: 86 5906 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 2379 4121 2379 Size: 1742 4250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI