(Circular) (Six-base) MAPSORT of: pFN21AB6691.seq Check: 9590 from: 1 to: 5989 pFN21AB6691 5989 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2512 2512 Size: 5989 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2340 2436 2912 3008 4052 5436 5704 Size: 404 304 96 476 96 1044 1384 268 Cuts at: 5704 1632 Size: 1917 Fragments arranged by size: 1917 1384 1044 476 404 304 268 96 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 2625 1166 Size: 1459 4530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5989 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3032 3064 3032 Size: 32 5957 AlwNI CAG_nnn'CTG Cuts at: 2920 3732 5304 2920 Size: 812 1572 3605 Fragments arranged by size: 3605 1572 812 ApaI G_GGCC'C Cuts at: 2661 2661 Size: 5989 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 3298 3524 3676 5785 1493 Size: 1805 226 152 2109 1697 Fragments arranged by size: 2109 1805 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2165 3314 1870 Size: 73 222 1149 4545 Fragments arranged by size: 4545 1149 222 73 BamHI G'GATC_C Cuts at: 2104 3319 3686 2104 Size: 1215 367 4407 Fragments arranged by size: 4407 1215 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3310 4765 618 Size: 325 217 627 1523 1455 1842 Fragments arranged by size: 1842 1523 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2661 2709 3308 1391 Size: 1270 48 599 4072 Fragments arranged by size: 4072 1270 599 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 928 Size: 971 424 4594 Fragments arranged by size: 4594 971 424 BbvCI CC'TCA_GC Cuts at: 2762 2762 Size: 5989 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3007 3041 4180 4214 1903 Size: 34 1070 34 1139 34 3678 Fragments arranged by size: 3678 1139 1070 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4600 136 Size: 107 122 71 631 605 2928 1525 Fragments arranged by size: 2928 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5984 5984 Size: 5989 BlpI GC'TnA_GC Cuts at: 3406 3406 Size: 5989 Bme1580I G_kGCm'C Cuts at: 1790 2661 4050 5206 1790 Size: 871 1389 1156 2573 Fragments arranged by size: 2573 1389 1156 871 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2595 3720 4678 Size: 416 53 83 399 24 1178 1125 958 Cuts at: 4678 442 Size: 1753 Fragments arranged by size: 1753 1178 1125 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2723 2108 Size: 615 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2411 4649 1239 Size: 1044 128 2238 2579 Fragments arranged by size: 2579 2238 1044 128 Bpu10I CC'TnA_GC Cuts at: 2762 2762 Size: 5989 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4114 4979 5277 4114 Size: 865 298 4826 Fragments arranged by size: 4826 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 3270 3796 4178 Size: 53 83 186 936 609 1128 526 382 Cuts at: 4178 4813 275 Size: 635 1451 Fragments arranged by size: 1451 1128 936 635 609 526 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5764 Fragments arranged by size: 5764 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2717 2759 1186 Size: 1531 42 4416 Fragments arranged by size: 4416 1531 42 BseYI C'CCAG_C Cuts at: 5192 5688 5192 Size: 496 5493 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5416 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2155 3439 4200 5228 1540 Size: 275 186 154 1284 761 1028 2301 Fragments arranged by size: 2301 1284 1028 761 275 186 154 BsiHKAI G_wGCw'C Cuts at: 2353 2709 2997 4050 4135 5206 2353 Size: 356 288 1053 85 1071 3136 Fragments arranged by size: 3136 1071 1053 356 288 85 BsmI GAATG_Cn' Cuts at: 2267 3269 3500 3593 2267 Size: 1002 231 93 4663 Fragments arranged by size: 4663 1002 231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2353 2661 2709 2997 3308 4050 4135 Size: 399 563 308 48 288 311 742 85 Cuts at: 4135 5206 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 563 399 311 308 288 85 48 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 2466 3878 5608 2466 Size: 1412 1730 2847 Fragments arranged by size: 2847 1730 1412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2512 2905 3344 844 Size: 1668 393 439 3489 Fragments arranged by size: 3489 1668 439 393 BsrBI CCG'CTC Cuts at: 1394 1823 2100 3876 1394 Size: 429 277 1776 3507 Fragments arranged by size: 3507 1776 429 277 BsrDI GCAATG_nn' Cuts at: 65 1248 3177 4484 4658 65 Size: 1183 1929 1307 174 1396 Fragments arranged by size: 1929 1396 1307 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4633 4820 1700 Size: 110 2823 187 2869 Fragments arranged by size: 2869 2823 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 2059 1925 Size: 134 5855 BssSI C'ACGA_G Cuts at: 4049 5061 4049 Size: 1012 4977 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5989 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstYI r'GATC_y Cuts at: 1972 2104 3319 3686 4071 4088 5540 5626 5638 Size: 132 1215 367 385 17 1452 86 12 Cuts at: 5638 5984 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1215 385 367 346 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2435 2435 Size: 5989 BtgI C'CryG_G Cuts at: 513 1065 2004 3006 3066 513 Size: 552 939 1002 60 3436 Fragments arranged by size: 3436 1002 939 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2200 487 Size: 683 56 974 4276 Fragments arranged by size: 4276 974 683 56 BtsI GCAGTG_nn' Cuts at: 3505 4299 4319 3505 Size: 794 20 5175 Fragments arranged by size: 5175 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DraIII CAC_nnn'GTG Cuts at: 2124 2124 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2243 2922 3436 Size: 54 1425 50 275 18 413 679 514 Cuts at: 3436 4325 5954 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 679 514 413 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 3919 1353 Size: 746 1820 3423 Fragments arranged by size: 3423 1820 746 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4576 4950 5096 387 Size: 1064 138 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2707 2707 Size: 5989 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2283 2340 2411 2436 2912 3008 Size: 393 404 247 57 71 25 476 96 Cuts at: 3008 4052 4649 5436 5704 1239 Size: 1044 597 787 268 1524 Fragments arranged by size: 1524 1044 787 597 476 404 393 268 247 96 71 57 25 EcoNI CCTnn'n_nnAGG Cuts at: 3035 3035 Size: 5989 EcoO109I rG'GnC_Cy Cuts at: 2431 2658 2431 Size: 227 5762 EcoRI G'AATT_C Cuts at: 3298 3676 3298 Size: 378 5611 EcoRV GAT'ATC Cuts at: 2294 2971 2294 Size: 677 5312 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 2752 5136 1678 Size: 1074 2384 2531 Fragments arranged by size: 2531 2384 1074 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 4630 4662 4704 Size: 32 135 32 806 32 2220 32 42 Cuts at: 4704 4736 1373 Size: 32 2626 Fragments arranged by size: 2626 2220 806 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3333 3579 669 Size: 988 281 1395 246 3079 Fragments arranged by size: 3079 1395 988 281 246 HindIII A'AGCT_T Cuts at: 748 2238 2369 748 Size: 1490 131 4368 Fragments arranged by size: 4368 1490 131 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3015 5103 5287 1305 Size: 1710 2088 184 2007 Fragments arranged by size: 2088 2007 1710 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2245 2924 10 Size: 54 1425 343 413 679 3075 Fragments arranged by size: 3075 1425 679 413 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2625 2693 3947 4306 4465 518 Size: 648 1459 68 1254 359 159 2042 Fragments arranged by size: 2042 1459 1254 648 359 159 68 MspA1I CmG'CkG Cuts at: 1606 2014 2074 2157 2497 2625 2761 2986 3405 Size: 408 60 83 340 128 136 225 419 Cuts at: 3405 4082 5230 5475 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 419 408 340 245 225 136 128 83 60 NcoI C'CATG_G Cuts at: 513 1065 2004 3006 3066 513 Size: 552 939 1002 60 3436 Fragments arranged by size: 3436 1002 939 552 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 3160 3347 4892 1132 Size: 97 1931 187 1545 2229 Fragments arranged by size: 2229 1931 1545 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5989 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5989 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5989 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PspOMI G'GGCC_C Cuts at: 2657 2657 Size: 5989 PstI C_TGCA'G Cuts at: 830 3144 3341 830 Size: 2314 197 3478 Fragments arranged by size: 3478 2314 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2497 2625 2761 2986 2497 Size: 128 136 225 5500 Fragments arranged by size: 5500 225 136 128 SacI G_AGCT'C Cuts at: 2709 2709 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 2774 4237 5949 2774 Size: 1463 1712 2814 Fragments arranged by size: 2814 1712 1463 SfcI C'TryA_G Cuts at: 826 1045 2215 3140 3337 4472 5153 5344 826 Size: 219 1170 925 197 1135 681 191 1471 Fragments arranged by size: 1471 1170 1135 925 681 219 197 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 3316 1872 Size: 1444 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4093 4994 5256 820 Size: 197 926 2150 901 262 1553 Fragments arranged by size: 2150 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 2401 3913 5 Size: 47 2349 1512 2081 Fragments arranged by size: 2349 2081 1512 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5989 StyI C'CwwG_G Cuts at: 513 1065 2004 2109 3006 3066 3428 513 Size: 552 939 105 897 60 362 3074 Fragments arranged by size: 3074 939 897 552 362 105 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4016 4033 4186 4371 4816 325 Size: 1255 2436 17 153 185 445 1498 Fragments arranged by size: 2436 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2772 4235 5947 Size: 274 80 33 51 1443 795 1463 1712 Cuts at: 5947 96 Size: 138 Fragments arranged by size: 1712 1463 1443 795 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5949 5981 5949 Size: 32 5957 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2719 2719 Size: 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 3314 1870 Size: 1444 4545 XmnI GAAnn'nnTTC Cuts at: 2961 4118 2961 Size: 1157 4832 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI