(Circular) (Six-base) MAPSORT of: pFN21AB6673.seq Check: 4546 from: 1 to: 5986 pFN21AB6673 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2282 2282 Size: 5986 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 2460 3307 2460 Size: 847 5139 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2383 4049 5433 5701 1632 Size: 751 1666 1384 268 1917 Fragments arranged by size: 1917 1666 1384 751 268 AfeI AGC'GCT Cuts at: 2255 2255 Size: 5986 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 2517 4885 5862 1203 Size: 1314 2368 977 1327 Fragments arranged by size: 2368 1327 1314 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 2089 2275 3729 5301 2089 Size: 186 1454 1572 2774 Fragments arranged by size: 2774 1572 1454 186 ApaI G_GGCC'C Cuts at: 2554 2554 Size: 5986 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 3295 3521 3673 5782 1493 Size: 1802 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2319 2361 2609 3092 3311 1870 Size: 73 376 42 248 483 219 4545 Fragments arranged by size: 4545 483 376 248 219 73 42 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2460 2575 3189 3218 Size: 325 217 627 539 134 115 614 29 Cuts at: 3218 3307 4762 618 Size: 89 1455 1842 Fragments arranged by size: 1842 1455 627 614 539 325 217 134 115 89 29 BanII G_rGCy'C Cuts at: 1391 2554 2568 2845 3305 1391 Size: 1163 14 277 460 4072 Fragments arranged by size: 4072 1163 460 277 14 BbeI G_GCGC'C Cuts at: 3222 3222 Size: 5986 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 3129 4597 Size: 107 122 71 631 605 652 805 1468 Cuts at: 4597 136 Size: 1525 Fragments arranged by size: 1525 1468 805 652 631 605 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 2268 3227 3403 2268 Size: 959 176 4851 Fragments arranged by size: 4851 959 176 Bme1580I G_kGCm'C Cuts at: 1790 2554 2580 4047 5203 1790 Size: 764 26 1467 1156 2573 Fragments arranged by size: 2573 1467 1156 764 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2512 2537 3717 Size: 416 53 83 399 24 1095 25 1180 Cuts at: 3717 4675 442 Size: 958 1753 Fragments arranged by size: 1753 1180 1095 958 416 399 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2528 2732 2972 4646 1239 Size: 1289 204 240 1674 2579 Fragments arranged by size: 2579 1674 1289 240 204 Bpu10I CC'TnA_GC Cuts at: 3119 3119 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 4111 4976 5274 2174 Size: 1937 865 298 2886 Fragments arranged by size: 2886 1937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2021 4649 882 Size: 617 522 2628 2219 Fragments arranged by size: 2628 2219 617 522 BsaAI yAC'GTr Cuts at: 493 1204 2154 2686 2848 3181 3777 493 Size: 711 950 532 162 333 596 2702 Fragments arranged by size: 2702 950 711 596 532 333 162 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3219 3793 4175 4810 Size: 53 83 186 936 1686 574 382 635 Cuts at: 4810 275 Size: 1451 Fragments arranged by size: 1686 1451 936 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2882 4419 4817 5091 5238 5649 2882 Size: 1537 398 274 147 411 3219 Fragments arranged by size: 3219 1537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2504 2534 2705 2735 1178 Size: 30 165 30 1101 30 171 30 4429 Fragments arranged by size: 4429 1101 171 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2639 3221 1186 Size: 1453 582 3951 Fragments arranged by size: 3951 1453 582 BseYI C'CCAG_C Cuts at: 2020 2538 2650 2946 3214 5189 5685 2020 Size: 518 112 296 268 1975 496 2321 Fragments arranged by size: 2321 1975 518 496 296 268 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2405 2528 2708 1163 Size: 573 669 123 180 4441 Fragments arranged by size: 4441 669 573 180 123 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2371 3436 4197 5225 1540 Size: 275 186 370 1065 761 1028 2301 Fragments arranged by size: 2301 1065 1028 761 370 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2139 4047 4132 5203 2121 Size: 18 1908 85 1071 2904 Fragments arranged by size: 2904 1908 1071 85 18 BsmI GAATG_Cn' Cuts at: 2814 3497 3590 2814 Size: 683 93 5210 Fragments arranged by size: 5210 683 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2139 2554 2568 2580 2845 3305 Size: 399 331 18 415 14 12 265 460 Cuts at: 3305 4047 4132 5203 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 415 399 331 265 85 18 14 12 BspEI T'CCGG_A Cuts at: 2882 5649 2882 Size: 2767 3219 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2206 2282 3341 844 Size: 1362 76 1059 3489 Fragments arranged by size: 3489 1362 1059 76 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2809 4481 4655 65 Size: 1183 1561 1672 174 1396 Fragments arranged by size: 1672 1561 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2532 2789 4630 4817 1700 Size: 110 722 257 1841 187 2869 Fragments arranged by size: 2869 1841 722 257 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 2686 794 Size: 1892 4094 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2950 2950 Size: 5986 BstYI r'GATC_y Cuts at: 1972 3316 3683 4068 4085 5537 5623 5635 5981 Size: 1344 367 385 17 1452 86 12 346 Cuts at: 5981 1972 Size: 1977 Fragments arranged by size: 1977 1452 1344 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2418 2568 2899 3185 513 Size: 552 1353 150 331 286 3314 Fragments arranged by size: 3314 1353 552 331 286 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3502 4296 4316 3502 Size: 794 20 5172 Fragments arranged by size: 5172 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DraIII CAC_nnn'GTG Cuts at: 2506 2984 2506 Size: 478 5508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 2368 2421 Size: 54 1425 50 275 18 331 207 53 Cuts at: 2421 2975 3433 4322 5951 8 Size: 554 458 889 1629 43 Fragments arranged by size: 1629 1425 889 554 458 331 275 207 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2368 3433 1537 Size: 275 556 1065 4090 Fragments arranged by size: 4090 1065 556 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3053 3916 1353 Size: 1700 863 3423 Fragments arranged by size: 3423 1700 863 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 3065 4573 4947 5093 387 Size: 1064 138 660 816 1508 374 146 1280 Fragments arranged by size: 1508 1280 1064 816 660 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2383 2528 2732 2972 4049 4646 5433 Size: 393 751 145 204 240 1077 597 787 Cuts at: 5433 5701 1239 Size: 268 1524 Fragments arranged by size: 1524 1077 787 751 597 393 268 240 204 145 EcoNI CCTnn'n_nnAGG Cuts at: 3165 3165 Size: 5986 EcoO109I rG'GnC_Cy Cuts at: 2550 2551 2890 3028 3158 2550 Size: 1 339 138 130 5378 Fragments arranged by size: 5378 339 138 130 1 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 5986 FseI GG_CCGG'CC Cuts at: 2536 2536 Size: 5986 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 2257 3222 5133 1678 Size: 579 965 1911 2531 Fragments arranged by size: 2531 1911 965 579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2215 2247 2473 Size: 32 135 32 427 32 184 32 226 Cuts at: 2473 2504 2505 2536 2705 2737 4627 4659 4701 Size: 31 1 31 169 32 1890 32 42 Cuts at: 4701 4733 1373 Size: 32 2626 Fragments arranged by size: 2626 1890 427 226 184 169 135 42 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KasI G'GCGC_C Cuts at: 3218 3218 Size: 5986 KpnI G_GTAC'C Cuts at: 2464 3311 2464 Size: 847 5139 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 2517 5862 2517 Size: 3345 2641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2423 2977 10 Size: 54 1425 343 591 554 3019 Fragments arranged by size: 3019 1425 591 554 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 2573 3944 4303 4462 518 Size: 648 1257 150 1371 359 159 2042 Fragments arranged by size: 2042 1371 1257 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2950 3187 3402 4079 5227 5472 1606 Size: 1344 237 215 677 1148 245 2120 Fragments arranged by size: 2120 1344 1148 677 245 237 215 NaeI GCC'GGC Cuts at: 2534 2791 2534 Size: 257 5729 NarI GG'CG_CC Cuts at: 3219 3219 Size: 5986 NcoI C'CATG_G Cuts at: 513 1065 2418 2568 513 Size: 552 1353 150 3931 Fragments arranged by size: 3931 1353 552 150 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NgoMIV G'CCGG_C Cuts at: 2532 2789 2532 Size: 257 5729 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NruI TCG'CGA Cuts at: 3087 3087 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2782 1108 Size: 1674 4312 PfoI T'CCnGG_A Cuts at: 2919 2919 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PmlI CAC'GTG Cuts at: 2154 2686 2848 3181 2154 Size: 532 162 333 4959 Fragments arranged by size: 4959 532 333 162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2890 3028 3158 2890 Size: 138 130 5718 Fragments arranged by size: 5718 138 130 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PspOMI G'GGCC_C Cuts at: 2550 2550 Size: 5986 PstI C_TGCA'G Cuts at: 830 2061 2208 2682 3338 830 Size: 1231 147 474 656 3478 Fragments arranged by size: 3478 1231 656 474 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2950 2950 Size: 5986 SacII CC_GC'GG Cuts at: 3188 3188 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SanDI GG'GwC_CC Cuts at: 2890 3028 2890 Size: 138 5848 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SexAI A'CCwGG_T Cuts at: 2595 2595 Size: 5986 SfcI C'TryA_G Cuts at: 826 1045 2057 2204 2678 3334 4469 5150 5341 Size: 219 1012 147 474 656 1135 681 191 Cuts at: 5341 826 Size: 1471 Fragments arranged by size: 1471 1135 1012 681 656 474 219 191 147 SfoI GGC'GCC Cuts at: 3220 3220 Size: 5986 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 2321 3313 1872 Size: 449 992 4545 Fragments arranged by size: 4545 992 449 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 4090 4991 5253 820 Size: 197 926 246 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 2063 2907 1517 Size: 546 844 4596 Fragments arranged by size: 4596 844 546 StyI C'CwwG_G Cuts at: 513 1065 2158 2418 2568 3425 513 Size: 552 1093 260 150 857 3074 Fragments arranged by size: 3074 1093 857 552 260 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2494 2960 4013 4030 4183 4368 4813 Size: 1255 914 466 1053 17 153 185 445 Cuts at: 4813 325 Size: 1498 Fragments arranged by size: 1498 1255 1053 914 466 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3066 4232 5944 Size: 274 80 33 51 1443 1089 1166 1712 Cuts at: 5944 96 Size: 138 Fragments arranged by size: 1712 1443 1166 1089 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2691 2723 5946 5978 2691 Size: 32 3223 32 2699 Fragments arranged by size: 3223 2699 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 2319 3311 1870 Size: 449 992 4545 Fragments arranged by size: 4545 992 449 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FspAI NsiI PacI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI XcmI