(Circular) (Six-base) MAPSORT of: pFN21AB6671.seq Check: 9587 from: 1 to: 5983 pFN21AB6671 5983 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2019 3326 1937 Size: 82 1307 4594 Fragments arranged by size: 4594 1307 82 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4046 5430 5698 1632 Size: 2414 1384 268 1917 Fragments arranged by size: 2414 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 2323 2754 4882 5859 1203 Size: 1120 431 2128 977 1327 Fragments arranged by size: 2128 1327 1120 977 431 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 2123 1166 Size: 957 5026 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3149 3169 3181 3201 3149 Size: 20 12 20 5931 Fragments arranged by size: 5931 20 20 12 AlwNI CAG_nnn'CTG Cuts at: 3174 3726 5298 3174 Size: 552 1572 3859 Fragments arranged by size: 3859 1572 552 ApaLI G'TGCA_C Cuts at: 2849 4040 5196 2849 Size: 1191 1156 3636 Fragments arranged by size: 3636 1191 1156 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 2229 3019 3308 1870 Size: 73 286 790 289 4545 Fragments arranged by size: 4545 790 289 286 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2690 2888 3304 4759 618 Size: 325 217 627 903 198 416 1455 1842 Fragments arranged by size: 1842 1455 903 627 416 325 217 198 BanII G_rGCy'C Cuts at: 1391 3302 1391 Size: 1911 4072 BbeI G_GCGC'C Cuts at: 2892 2892 Size: 5983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3113 928 Size: 971 1214 3798 Fragments arranged by size: 3798 1214 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2195 2210 2229 2244 4174 4208 1903 Size: 34 258 15 19 15 1930 34 3678 Fragments arranged by size: 3678 1930 258 34 34 19 15 15 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4594 136 Size: 107 122 71 631 605 2922 1525 Fragments arranged by size: 2922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2193 5978 2193 Size: 3785 2198 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2695 2853 3209 3281 4044 5200 1790 Size: 905 158 356 72 763 1156 2573 Fragments arranged by size: 2573 1156 905 763 356 158 72 BmgBI CAC'GTC Cuts at: 1318 2326 1318 Size: 1008 4975 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2290 2345 3212 Size: 416 53 83 399 24 873 55 867 Cuts at: 3212 3714 4672 442 Size: 502 958 1753 Fragments arranged by size: 1753 958 873 867 502 416 399 83 55 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2186 4643 1239 Size: 941 6 2457 2579 Fragments arranged by size: 2579 2457 941 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 4108 4973 5271 2243 Size: 1865 865 298 2955 Fragments arranged by size: 2955 1865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2737 2806 4646 882 Size: 617 1238 69 1840 2219 Fragments arranged by size: 2219 1840 1238 617 69 BsaAI yAC'GTr Cuts at: 493 1204 2224 2977 3774 493 Size: 711 1020 753 797 2702 Fragments arranged by size: 2702 1020 797 753 711 BsaBI GATnn'nnATC Cuts at: 3224 5977 3224 Size: 2753 3230 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 2889 3790 4172 Size: 53 83 186 936 483 873 901 382 Cuts at: 4172 4807 275 Size: 635 1451 Fragments arranged by size: 1451 936 901 873 635 483 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2045 2049 2075 2229 Size: 30 165 30 616 26 4 26 154 Cuts at: 2229 2234 2259 2264 2585 2615 3149 3179 3260 Size: 5 25 5 321 30 534 30 81 Cuts at: 3260 3290 1178 Size: 30 3871 Fragments arranged by size: 3871 616 534 321 165 154 81 30 30 30 30 30 26 26 25 5 5 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 3260 1186 Size: 973 1101 3909 Fragments arranged by size: 3909 1101 973 BseYI C'CCAG_C Cuts at: 2078 2087 5186 5682 2078 Size: 9 3099 496 2379 Fragments arranged by size: 3099 2379 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 2330 2817 1163 Size: 573 468 126 487 4329 Fragments arranged by size: 4329 573 487 468 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2358 2466 2853 4044 4129 5200 2358 Size: 108 387 1191 85 1071 3141 Fragments arranged by size: 3141 1191 1071 387 108 85 BsmI GAATG_Cn' Cuts at: 3494 3587 3494 Size: 93 5890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2358 2466 2695 2853 3209 3281 3302 Size: 399 568 108 229 158 356 72 21 Cuts at: 3302 4044 4129 5200 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 568 399 356 229 158 108 85 72 21 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 2788 3338 844 Size: 1508 436 550 3489 Fragments arranged by size: 3489 1508 550 436 BsrBI CCG'CTC Cuts at: 1394 1823 2010 2240 3870 1394 Size: 429 187 230 1630 3507 Fragments arranged by size: 3507 1630 429 230 187 BsrDI GCAATG_nn' Cuts at: 65 1248 3135 4478 4652 65 Size: 1183 1887 1343 174 1396 Fragments arranged by size: 1887 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3047 4627 4814 1700 Size: 110 1237 1580 187 2869 Fragments arranged by size: 2869 1580 1237 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 2498 4043 5055 2498 Size: 1545 1012 3426 Fragments arranged by size: 3426 1545 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2353 794 Size: 1559 4424 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 2980 1184 Size: 1796 4187 BstXI CCAn_nnnn'nTGG Cuts at: 2125 2125 Size: 5983 BstYI r'GATC_y Cuts at: 1972 2193 3225 3313 3680 4065 4082 5534 5620 Size: 221 1032 88 367 385 17 1452 86 Cuts at: 5620 5632 5978 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1032 385 367 346 221 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2910 2910 Size: 5983 BtgI C'CryG_G Cuts at: 513 1065 2139 2372 2526 3153 513 Size: 552 1074 233 154 627 3343 Fragments arranged by size: 3343 1074 627 552 233 154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 2615 3059 3266 3499 4293 4313 2615 Size: 444 207 233 794 20 4285 Fragments arranged by size: 4285 794 444 233 207 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DraIII CAC_nnn'GTG Cuts at: 2353 2980 2353 Size: 627 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2529 2634 Size: 54 1425 50 275 18 306 393 105 Cuts at: 2634 3430 4319 5948 8 Size: 796 889 1629 43 Fragments arranged by size: 1629 1425 889 796 393 306 275 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3913 1353 Size: 2560 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 4570 4944 5090 387 Size: 1064 138 451 2530 374 146 1280 Fragments arranged by size: 2530 1280 1064 451 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2186 4046 4643 5430 5698 1239 Size: 393 548 6 1860 597 787 268 1524 Fragments arranged by size: 1860 1524 787 597 548 393 268 6 EcoNI CCTnn'n_nnAGG Cuts at: 2586 2586 Size: 5983 EcoO109I rG'GnC_Cy Cuts at: 2914 2941 2914 Size: 27 5956 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 5983 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 2045 2892 5130 1678 Size: 367 847 2238 2531 Fragments arranged by size: 2531 2238 847 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2229 2234 2261 Size: 32 135 32 473 32 152 5 27 Cuts at: 2261 2266 2585 2617 2889 2921 3117 3149 4624 Size: 5 319 32 272 32 196 32 1475 Cuts at: 4624 4656 4698 4730 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1475 473 319 272 196 152 135 42 32 32 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KasI G'GCGC_C Cuts at: 2888 2888 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5097 5281 1305 Size: 3792 184 2007 Fragments arranged by size: 3792 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2636 10 Size: 54 1425 343 804 3357 Fragments arranged by size: 3357 1425 804 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2603 2996 3941 4300 4459 518 Size: 648 957 480 393 945 359 159 2042 Fragments arranged by size: 2042 957 945 648 480 393 359 159 MspA1I CmG'CkG Cuts at: 1606 2141 3399 4076 5224 5469 1606 Size: 535 1258 677 1148 245 2120 Fragments arranged by size: 2120 1258 1148 677 535 245 NarI GG'CG_CC Cuts at: 2889 2889 Size: 5983 NcoI C'CATG_G Cuts at: 513 1065 3153 513 Size: 552 2088 3343 Fragments arranged by size: 3343 2088 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2758 3001 3124 3341 4886 1132 Size: 97 1529 243 123 217 1545 2229 Fragments arranged by size: 2229 1545 1529 243 217 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5983 PciI A'CATG_T Cuts at: 2754 4882 2754 Size: 2128 3855 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 2742 1108 Size: 1524 110 4349 Fragments arranged by size: 4349 1524 110 PfoI T'CCnGG_A Cuts at: 2301 2625 2682 2772 2301 Size: 324 57 90 5512 Fragments arranged by size: 5512 324 90 57 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PmlI CAC'GTG Cuts at: 2224 2977 2224 Size: 753 5230 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2399 2431 3149 3181 4052 4084 5565 5597 2399 Size: 32 718 32 871 32 1481 32 2785 Fragments arranged by size: 2785 1481 871 718 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PstI C_TGCA'G Cuts at: 830 2562 2799 3069 3335 830 Size: 1732 237 270 266 3478 Fragments arranged by size: 3478 1732 270 266 237 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 SacII CC_GC'GG Cuts at: 2142 2142 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 2314 2558 2795 3065 3331 4466 5147 Size: 219 1269 244 237 270 266 1135 681 Cuts at: 5147 5338 826 Size: 191 1471 Fragments arranged by size: 1471 1269 1135 681 270 266 244 237 219 191 SfoI GGC'GCC Cuts at: 2890 2890 Size: 5983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 3047 1700 Size: 1347 4636 SmaI CCC'GGG Cuts at: 1872 3021 3310 1872 Size: 1149 289 4545 Fragments arranged by size: 4545 1149 289 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 4087 4988 5250 820 Size: 197 926 315 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 315 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3124 3341 1132 Size: 1992 217 3774 Fragments arranged by size: 3774 1992 217 SrfI GCCC'GGGC Cuts at: 3021 3021 Size: 5983 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 2786 1517 Size: 1269 4714 StyI C'CwwG_G Cuts at: 513 1065 2103 2670 3153 3422 513 Size: 552 1038 567 483 269 3074 Fragments arranged by size: 3074 1038 567 552 483 269 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2364 2534 2954 4010 4027 4180 4365 Size: 1255 784 170 420 1056 17 153 185 Cuts at: 4365 4810 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1056 784 445 420 185 170 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2539 4229 Size: 274 80 33 51 1443 447 115 1690 Cuts at: 4229 5941 96 Size: 1712 138 Fragments arranged by size: 1712 1690 1443 447 274 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 2270 2302 2518 2550 3260 3292 5943 Size: 32 219 32 216 32 710 32 2651 Cuts at: 5943 5975 2019 Size: 32 2027 Fragments arranged by size: 2651 2027 710 219 216 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2965 2965 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3019 3308 1870 Size: 1149 289 4545 Fragments arranged by size: 4545 1149 289 XmnI GAAnn'nnTTC Cuts at: 2195 4112 2195 Size: 1917 4066 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SwaI