Restriction map for
pFN21AB6669
(Circular) (Six-base) MAPSORT of: pFN21AB6669.seq Check: 1416 from: 1 to: 5980
pFN21AB6669 5980 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2481 2706 2792 3159 4043 5427 5695 1632
Size: 849 225 86 367 884 1384 268 1917
Fragments arranged by size:
1917 1384 884 849 367 268 225 86
AfeI AGC'GCT
Cuts at: 2178 3072 2178
Size: 894 5086
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 2417 4879 5856 1203
Size: 1214 2462 977 1327
Fragments arranged by size:
2462 1327 1214 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 2418 4709 2418
Size: 2291 3689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2870 3723 5295 2870
Size: 853 1572 3555
Fragments arranged by size:
3555 1572 853
ApaLI G'TGCA_C
Cuts at: 3238 4037 5193 3238
Size: 799 1156 4025
Fragments arranged by size:
4025 1156 799
ApoI r'AATT_y
Cuts at: 1493 2320 3082 3289 3515 3667 5776 1493
Size: 827 762 207 226 152 2109 1697
Fragments arranged by size:
2109 1697 827 762 226 207 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 3305 1870
Size: 73 101 1261 4545
Fragments arranged by size:
4545 1261 101 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 3301 4756 618
Size: 325 217 627 431 1083 1455 1842
Fragments arranged by size:
1842 1455 1083 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2928 2984 3299 1391
Size: 1537 56 315 4072
Fragments arranged by size:
4072 1537 315 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5009
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3119 3153 4171 4205 1903
Size: 34 1182 34 1018 34 3678
Fragments arranged by size:
3678 1182 1018 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2786 4591 136
Size: 107 122 71 631 605 1114 1805 1525
Fragments arranged by size:
1805 1525 1114 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2040 2607 5975 2040
Size: 567 3368 2045
Fragments arranged by size:
3368 2045 567
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 3242 4041 5197 1790
Size: 1452 799 1156 2573
Fragments arranged by size:
2573 1452 1156 799
BmgBI CAC'GTC
Cuts at: 1318 3237 1318
Size: 1919 4061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2463 3711 4669
Size: 416 53 83 399 24 1046 1248 958
Cuts at: 4669 442
Size: 1753
Fragments arranged by size:
1753 1248 1046 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2760 4640 1239
Size: 1521 1880 2579
Fragments arranged by size:
2579 1880 1521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 4105 4970 5268 2750
Size: 1355 865 298 3462
Fragments arranged by size:
3462 1355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3196 4643 882
Size: 617 1697 1447 2219
Fragments arranged by size:
2219 1697 1447 617
BsaAI yAC'GTr
Cuts at: 493 1204 2946 3771 493
Size: 711 1742 825 2702
Fragments arranged by size:
2702 1742 825 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 1186
Size: 1069 4911
BseYI C'CCAG_C
Cuts at: 5183 5679 5183
Size: 496 5484
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3087 3199 1163
Size: 573 1351 112 3944
Fragments arranged by size:
3944 1351 573 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2906 2963 2984 3242 4041 4126 5197 2034
Size: 872 57 21 258 799 85 1071 2817
Fragments arranged by size:
2817 1071 872 799 258 85 57 21
BsmI GAATG_Cn'
Cuts at: 3034 3491 3584 3034
Size: 457 93 5430
Fragments arranged by size:
5430 457 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2906 2928 2963 2984 3242 3299
Size: 399 244 872 22 35 21 258 57
Cuts at: 3299 4041 4126 5197 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 872 742 399 258 244 85
57 35 22 21
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2563 4475 4649 65
Size: 1183 1315 1912 174 1396
Fragments arranged by size:
1912 1396 1315 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 2110 96
Size: 2014 3966
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2040 2607 3310 3677 4062 4079 5531 5617
Size: 68 567 703 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 703 567 385 367 346 86
68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 3211 3496 4290 4310 2032
Size: 1179 285 794 20 3702
Fragments arranged by size:
3702 1179 794 285 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 2326 3284 2326
Size: 958 5022
DraIII CAC_nnn'GTG
Cuts at: 2234 2234
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945
Size: 54 1425 50 275 18 1597 889 1629
Cuts at: 5945 8
Size: 43
Fragments arranged by size:
1629 1597 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2619 3910 1353
Size: 1266 1291 3423
Fragments arranged by size:
3423 1291 1266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3007 4567 4941 5087 387
Size: 1064 138 1418 1560 374 146 1280
Fragments arranged by size:
1560 1418 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2982 2982
Size: 5980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2481 2706 2760 2792 3159 4043 4640
Size: 393 849 225 54 32 367 884 597
Cuts at: 4640 5427 5695 1239
Size: 787 268 1524
Fragments arranged by size:
1524 884 849 787 597 393 367 268
225 54 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2919 2919
Size: 5980
EcoO109I rG'GnC_Cy
Cuts at: 2487 2487
Size: 5980
EcoRI G'AATT_C
Cuts at: 3082 3289 3667 3082
Size: 207 378 5395
Fragments arranged by size:
5395 378 207
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2180 3074 5127 1678
Size: 502 894 2053 2531
Fragments arranged by size:
2531 2053 894 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2404 2436 4621 4653 4695
Size: 32 135 32 832 32 2185 32 42
Cuts at: 4695 4727 1373
Size: 32 2626
Fragments arranged by size:
2626 2185 832 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3197 748
Size: 2449 3531
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2056 5094 5278 1305
Size: 751 3038 184 2007
Fragments arranged by size:
3038 2007 751 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2873 3938 4297 4456 518
Size: 648 1707 1065 359 159 2042
Fragments arranged by size:
2042 1707 1065 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2674 2884 3396 4073 5221 5466 1606
Size: 1068 210 512 677 1148 245 2120
Fragments arranged by size:
2120 1148 1068 677 512 245 210
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2421 3338 4883 1132
Size: 97 1192 917 1545 2229
Fragments arranged by size:
2229 1545 1192 917 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 2417 4879 2417
Size: 2462 3518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PfoI T'CCnGG_A
Cuts at: 2760 2760
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2487 2487
Size: 5980
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 2309 3550 2309
Size: 1241 4739
PstI C_TGCA'G
Cuts at: 830 2484 3332 830
Size: 1654 848 3478
Fragments arranged by size:
3478 1654 848
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2674 2674
Size: 5980
SacI G_AGCT'C
Cuts at: 2984 2984
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2480 2491 3328 4463 5144 5335 826
Size: 219 1435 11 837 1135 681 191 1471
Fragments arranged by size:
1471 1435 1135 837 681 219 191 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 4084 4985 5247 820
Size: 197 926 786 1355 901 262 1553
Fragments arranged by size:
1553 1355 926 901 786 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 2660 3904 5
Size: 47 2608 1244 2081
Fragments arranged by size:
2608 2081 1244 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 2985 3419 513
Size: 552 939 981 434 3074
Fragments arranged by size:
3074 981 939 552 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2860 2946 4007 4024 4177 4362 4807
Size: 1255 1280 86 1061 17 153 185 445
Cuts at: 4807 325
Size: 1498
Fragments arranged by size:
1498 1280 1255 1061 445 185 153 86
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2110 4226 5938
Size: 274 80 33 51 1443 133 2116 1712
Cuts at: 5938 96
Size: 138
Fragments arranged by size:
2116 1712 1443 274 138 133 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3232 3264 5940 5972 3232
Size: 32 2676 32 3240
Fragments arranged by size:
3240 2676 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 2805 4109 2805
Size: 1304 4676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI