(Circular) (Six-base) MAPSORT of: pFN21AB6660.seq Check: 7675 from: 1 to: 6058 pFN21AB6660 6058 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2087 2087 Size: 6058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 1937 Size: 1464 4594 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 4121 5505 5773 1632 Size: 585 1904 1384 268 1917 Fragments arranged by size: 1917 1904 1384 585 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 4957 5934 1203 Size: 3754 977 1327 Fragments arranged by size: 3754 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 2210 2932 4787 2210 Size: 722 1855 3481 Fragments arranged by size: 3481 1855 722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2589 1184 Size: 1405 4653 AlwNI CAG_nnn'CTG Cuts at: 3801 5373 3801 Size: 1572 4486 ApaI G_GGCC'C Cuts at: 2477 2477 Size: 6058 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 2354 3074 3182 3350 3367 3593 3745 5854 Size: 861 720 108 168 17 226 152 2109 Cuts at: 5854 1493 Size: 1697 Fragments arranged by size: 2109 1697 861 720 226 168 152 108 17 AseI AT'TA_AT Cuts at: 160 1032 2264 4612 160 Size: 872 1232 2348 1606 Fragments arranged by size: 2348 1606 1232 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 3383 1870 Size: 73 1440 4545 Fragments arranged by size: 4545 1440 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 4834 618 Size: 325 217 627 1592 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2477 3377 1391 Size: 1086 900 4072 Fragments arranged by size: 4072 1086 900 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 2300 2931 928 Size: 971 345 56 631 4055 Fragments arranged by size: 4055 971 631 345 56 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4249 4283 1903 Size: 34 50 34 2228 34 3678 Fragments arranged by size: 3678 2228 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BfrBI ATG'CAT Cuts at: 2141 2803 2141 Size: 662 5396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4669 136 Size: 107 122 71 631 605 2997 1525 Fragments arranged by size: 2997 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 3475 3475 Size: 6058 Bme1580I G_kGCm'C Cuts at: 1790 2477 4119 5275 1790 Size: 687 1642 1156 2573 Fragments arranged by size: 2573 1642 1156 687 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3209 3789 4747 Size: 416 53 83 399 24 1792 580 958 Cuts at: 4747 442 Size: 1753 Fragments arranged by size: 1792 1753 958 580 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4718 1239 Size: 3479 2579 Bpu10I CC'TnA_GC Cuts at: 2951 2951 Size: 6058 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2217 2816 4183 5048 5346 2217 Size: 599 1367 865 298 2929 Fragments arranged by size: 2929 1367 865 599 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4721 882 Size: 617 3222 2219 Fragments arranged by size: 3222 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3865 4247 4882 275 Size: 53 83 186 936 2332 382 635 1451 Fragments arranged by size: 2332 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5833 Fragments arranged by size: 5833 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2816 1186 Size: 1630 4428 BseYI C'CCAG_C Cuts at: 2470 3105 5261 5757 2470 Size: 635 2156 496 2771 Fragments arranged by size: 2771 2156 635 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2454 1163 Size: 573 718 4767 Fragments arranged by size: 4767 718 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4119 4204 5275 4119 Size: 85 1071 4902 Fragments arranged by size: 4902 1071 85 BsmI GAATG_Cn' Cuts at: 3569 3662 3569 Size: 93 5965 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2477 3377 4119 4204 5275 1391 Size: 399 687 900 742 85 1071 2174 Fragments arranged by size: 2174 1071 900 742 687 399 85 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2087 3413 844 Size: 1243 1326 3489 Fragments arranged by size: 3489 1326 1243 BsrBI CCG'CTC Cuts at: 1394 1823 3945 1394 Size: 429 2122 3507 Fragments arranged by size: 3507 2122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2161 4553 4727 65 Size: 1183 913 2392 174 1396 Fragments arranged by size: 2392 1396 1183 913 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4702 4889 1700 Size: 110 2892 187 2869 Fragments arranged by size: 2892 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 4118 5130 4118 Size: 1012 5046 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6058 BstBI TT'CG_AA Cuts at: 3190 3264 4862 3190 Size: 74 1598 4386 Fragments arranged by size: 4386 1598 74 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2168 2168 Size: 6058 BstYI r'GATC_y Cuts at: 1972 3388 3755 4140 4157 5609 5695 5707 6053 Size: 1416 367 385 17 1452 86 12 346 Cuts at: 6053 1972 Size: 1977 Fragments arranged by size: 1977 1452 1416 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3032 3032 Size: 6058 BtgI C'CryG_G Cuts at: 513 1065 2038 513 Size: 552 973 4533 Fragments arranged by size: 4533 973 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 2053 2173 3045 3574 4368 4388 2053 Size: 120 872 529 794 20 3723 Fragments arranged by size: 3723 872 794 529 120 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 2892 3505 Size: 54 1425 50 275 18 271 791 613 Cuts at: 3505 4394 6023 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 791 613 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2487 2742 3988 1353 Size: 1134 255 1246 3423 Fragments arranged by size: 3423 1246 1134 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 4121 4718 5505 5773 1239 Size: 393 585 1904 597 787 268 1524 Fragments arranged by size: 1904 1524 787 597 585 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2219 2219 Size: 6058 EcoO109I rG'GnC_Cy Cuts at: 2474 2955 2474 Size: 481 5577 EcoRI G'AATT_C Cuts at: 3182 3350 3367 3745 3182 Size: 168 17 378 5495 Fragments arranged by size: 5495 378 168 17 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2327 2327 Size: 6058 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 5205 1678 Size: 3527 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2196 2228 2918 2950 4699 Size: 32 135 32 624 32 690 32 1749 Cuts at: 4699 4731 4773 4805 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1749 690 624 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 2792 2813 748 Size: 2044 21 3993 Fragments arranged by size: 3993 2044 21 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 2145 3657 2145 Size: 1512 4546 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3083 3325 5172 5356 1305 Size: 1778 242 1847 184 2007 Fragments arranged by size: 2007 1847 1778 242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2103 2894 10 Size: 54 1425 343 271 791 3174 Fragments arranged by size: 3174 1425 791 343 271 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 2166 3123 4016 4375 4534 518 Size: 648 946 54 957 893 359 159 2042 Fragments arranged by size: 2042 957 946 893 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2040 2438 2470 3010 3474 4151 5299 5544 Size: 434 398 32 540 464 677 1148 245 Cuts at: 5544 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 540 464 434 398 245 32 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5506 NdeI CA'TA_TG Cuts at: 387 2765 387 Size: 2378 3680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NsiI A_TGCA'T Cuts at: 2143 2805 2143 Size: 662 5396 NspI r_CATG'y Cuts at: 1132 1229 3094 3416 4961 1132 Size: 97 1865 322 1545 2229 Fragments arranged by size: 2229 1865 1545 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 4957 4957 Size: 6058 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6058 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PspOMI G'GGCC_C Cuts at: 2473 2473 Size: 6058 PstI C_TGCA'G Cuts at: 830 2443 3289 3410 830 Size: 1613 846 121 3478 Fragments arranged by size: 3478 1613 846 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 2438 2470 2438 Size: 32 6026 SacII CC_GC'GG Cuts at: 2041 2041 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2439 3285 3406 4541 5222 5413 826 Size: 219 1394 846 121 1135 681 191 1471 Fragments arranged by size: 1471 1394 1135 846 681 219 191 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 3385 1872 Size: 1513 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2232 2795 4162 5063 5325 820 Size: 197 926 289 563 1367 901 262 1553 Fragments arranged by size: 1553 1367 926 901 563 289 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 2958 3497 513 Size: 552 1893 539 3074 Fragments arranged by size: 3074 1893 552 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4085 4102 4255 4440 4885 325 Size: 1255 2505 17 153 185 445 1498 Fragments arranged by size: 2505 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 4304 6016 Size: 274 80 33 51 1443 270 2057 1712 Cuts at: 6016 96 Size: 138 Fragments arranged by size: 2057 1712 1443 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3201 3233 6018 6050 3201 Size: 32 2785 32 3209 Fragments arranged by size: 3209 2785 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 3383 1870 Size: 1513 4545 XmnI GAAnn'nnTTC Cuts at: 2358 3186 4187 2358 Size: 828 1001 4229 Fragments arranged by size: 4229 1001 828 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI