Restriction map for
pFN21AB6656
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# NO CUT ENZYMES ( 123 enzymes) #
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AccI AccIII AclI AflII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MfeI MflI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1180 GACGTC GACGT^C
Acc65I 220 GGTACC G^GTACC
AccII 494 CGCG CG^CG
AflIII 1103 ACGTGT A^C[GA][CT]GT
AocI 1063 CCTGAGG CC^T[ACGT]AGG
ApaI 563 GGGCCC GGGCC^C
Asp700I 1080 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 220 GGTACC G^GTACC
AspI 1180 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1190 CTCGGG C^[CT]CG[GA]G
BcnI 337 CCCGG CC^[GC]GG
BpmI 913 CTGGAG CTGGAG(16/14)
BsaWI 28 TCCGGT [AT]^CCGG[AT]
BsmFI 1186 GTCCC GGGAC(10/14)
BsoBI 1190 CTCGGG C^[CT]CG[GA]G
BsrBI 277 GAGCGG CCGCTC(-3/-3)
BsrDI 953 GCAATG GCAATG(2/0)
BssSI 1336 CACGAG CACGAG(-5/-1)
BstAPI 75 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 494 CGCG CG^CG
BstXI 1045 CCAGTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1063 CCTGAGG CC^T[ACGT]AGG
CfoI 495 GCGC GCG^C
DraIII 348 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1063 CCTGAGG CC^T[ACGT]AGG
EcoRI 1166 GAATTC G^AATTC
HhaI 495 GCGC GCG^C
HinP1I 495 GCGC G^CGC
KpnI 220 GGTACC GGTAC^C
MslI 108 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 494 CGCG CG^CG
NciI 337 CCCGG CC^[GC]GG
NcoI 902 CCATGG C^CATGG
PflFI 1180 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 485 GACTC GAGTC(4/5)
PshAI 994 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 592 CTGCAG CTGCA^G
PvuII 451 CAGCTG CAG^CTG
SexAI 740 ACCTGGT A^CC[AT]GGT
TfiI 1118 GAATC G^A[AT]TC
Tth111I 1180 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1080 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcsI 1084 AAATTC [GA]^AATT[CT]
1166 GAATTC
ApoI 1084 AAATTC [GA]^AATT[CT]
1166 GAATTC
AvaII 78 GGACC G^G[AT]CC
129 GGACC
BanI 220 GGTACC G^G[CT][GA]CC
1268 GGCACC
BanII 563 GGGCCC G[GA]GC[CT]^C
781 GGGCTC
BcgI 678 CGACGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
920 GCAACAATTTCG
BfaI 839 CTAG C^TAG
886 CTAG
BlpI 274 GCTGAGC GC^T[ACGT]AGC
411 GCTCAGC
Bpu1102I 274 GCTGAGC GC^T[ACGT]AGC
411 GCTCAGC
BsaI 938 GAGACC GGTCTC(1/5)
1000 GGTCTC
BsgI 1342 CTGCAC GTGCAG(16/14)
745 GTGCAG
BsmBI 98 GAGACG CGTCTC(1/5)
620 GAGACG
BspHI 716 TCATGA T^CATGA
1253 TCATGA
BspMI 80 ACCTGC ACCTGC(4/8)
830 ACCTGC
BsrI 137 ACTGG ACTGG(1/-1)
1045 CCAGT
CelII 274 GCTGAGC GC^T[ACGT]AGC
411 GCTCAGC
DpnII 258 GATC ^GATC
762 GATC
HinfI 485 GACTC G^A[ACGT]TC
1118 GAATC
MaeI 839 CTAG C^TAG
886 CTAG
MaeIII 121 GTCAC ^GT[ACGT]AC
793 GTCAC
MboI 258 GATC ^GATC
762 GATC
MboII 1080 GAAGA GAAGA(8/7)
1257 GAAGA
MseI 503 TTAA T^TAA
671 TTAA
MspA1I 451 CAGCTG C[AC]G^C[GT]G
555 CAGCGG
NdeII 258 GATC ^GATC
762 GATC
PpuMI 77 AGGACCT [GA]G^G[AT]CC[CT]
128 AGGACCC
RcaI 716 TCATGA T^CATGA
1253 TCATGA
Sau3AI 258 GATC ^GATC
762 GATC
SfcI 592 CTGCAG C^T[GA][CT]AG
1300 CTACAG
SmlI 874 CTCAAG C^T[CT][GA]AG
1318 CTTGAG
StuI 303 AGGCCT AGG^CCT
692 AGGCCT
TaqI 419 TCGA T^CGA
936 TCGA
Tru9I 503 TTAA T^TAA
671 TTAA
Tsp45I 121 GTCAC ^GT[GC]AC
793 GTCAC
TthHB8I 419 TCGA T^CGA
936 TCGA
XcmI 149 CCAAAAACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
902 CCATGGAATACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI BalI BbvI BglI BmyI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI Hin1I HpaII HphI ItaI
MaeII MluNI MnlI MscI MspI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI StyI TseI
Tsp509I TspRI