(Circular) (Six-base) MAPSORT of: pFN21AB6656.seq Check: 4961 from: 1 to: 6055 pFN21AB6656 6055 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3189 3865 278 Size: 53 83 186 936 1653 676 2468 Fragments arranged by size: 2468 1653 936 676 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 2225 3376 2225 Size: 1151 4904 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 2730 3200 4118 5502 5770 1632 Size: 977 121 470 918 1384 268 1917 Fragments arranged by size: 1917 1384 977 918 470 268 121 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 3108 4954 5931 1203 Size: 1905 1846 977 1327 Fragments arranged by size: 1905 1846 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2117 1166 Size: 951 5104 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2765 1184 Size: 1581 4474 AlwNI CAG_nnn'CTG Cuts at: 2086 2565 2758 3068 3798 5370 2086 Size: 479 193 310 730 1572 2771 Fragments arranged by size: 2771 1572 730 479 310 193 ApaI G_GGCC'C Cuts at: 2572 2572 Size: 6055 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 3089 3171 3364 3590 3742 5851 1493 Size: 1596 82 193 226 152 2109 1697 Fragments arranged by size: 2109 1697 1596 226 193 152 82 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 3195 3380 1870 Size: 73 1252 185 4545 Fragments arranged by size: 4545 1252 185 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2225 3273 3376 4831 618 Size: 325 217 627 438 1048 103 1455 1842 Fragments arranged by size: 1842 1455 1048 627 438 325 217 103 BanII G_rGCy'C Cuts at: 1391 2572 2790 3374 1391 Size: 1181 218 584 4072 Fragments arranged by size: 4072 1181 584 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5084 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2672 2706 2914 2948 4246 4280 1903 Size: 34 735 34 208 34 1298 34 3678 Fragments arranged by size: 3678 1298 735 208 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2175 2705 3071 Size: 107 122 71 631 605 503 530 366 Cuts at: 3071 4666 136 Size: 1595 1525 Fragments arranged by size: 1595 1525 631 605 530 503 366 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 2280 2417 3472 2280 Size: 137 1055 4863 Fragments arranged by size: 4863 1055 137 Bme1580I G_kGCm'C Cuts at: 1790 2531 2572 4116 5272 1790 Size: 741 41 1544 1156 2573 Fragments arranged by size: 2573 1544 1156 741 41 BmgBI CAC'GTC Cuts at: 1318 2107 2629 3109 1318 Size: 789 522 480 4264 Fragments arranged by size: 4264 789 522 480 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3786 4744 442 Size: 416 53 83 399 24 2369 958 1753 Fragments arranged by size: 2369 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2126 2891 2126 Size: 765 5290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2939 4715 1239 Size: 1700 1776 2579 Fragments arranged by size: 2579 1776 1700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2864 3344 4180 5045 5343 2864 Size: 480 836 865 298 3576 Fragments arranged by size: 3576 865 836 480 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2937 3011 4718 882 Size: 617 1438 74 1707 2219 Fragments arranged by size: 2219 1707 1438 617 74 BsaAI yAC'GTr Cuts at: 493 1204 3846 493 Size: 711 2642 2702 Fragments arranged by size: 2702 2642 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2685 3020 3186 3282 Size: 53 83 186 936 1152 335 166 96 Cuts at: 3282 3862 4244 4879 275 Size: 580 382 635 1451 Fragments arranged by size: 1451 1152 936 635 580 382 335 186 166 96 83 53 BsaWI w'CCGG_w Cuts at: 2033 4488 4886 5160 5307 5718 2033 Size: 2455 398 274 147 411 2370 Fragments arranged by size: 2455 2370 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5830 Fragments arranged by size: 5830 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2789 2867 1186 Size: 1258 345 78 4374 Fragments arranged by size: 4374 1258 345 78 BseYI C'CCAG_C Cuts at: 2185 2565 2635 2860 5258 5754 2185 Size: 380 70 225 2398 496 2486 Fragments arranged by size: 2486 2398 496 380 225 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2771 3332 1163 Size: 573 1035 561 3886 Fragments arranged by size: 3886 1035 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2112 2333 2388 2469 3330 4116 4201 5272 2112 Size: 221 55 81 861 786 85 1071 2895 Fragments arranged by size: 2895 1071 861 786 221 85 81 55 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 BsmBI CGTCTCn'nnnn_ Cuts at: 2097 2619 2097 Size: 522 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2333 2388 2469 2531 2572 2790 Size: 399 322 221 55 81 62 41 218 Cuts at: 2790 3330 3374 4116 4201 5272 1391 Size: 540 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 399 322 221 218 85 81 62 55 44 41 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 2721 3258 3944 5674 2721 Size: 537 686 1730 3102 Fragments arranged by size: 3102 1730 686 537 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2844 3410 844 Size: 1250 750 566 3489 Fragments arranged by size: 3489 1250 750 566 BsrBI CCG'CTC Cuts at: 1394 1823 2284 3942 1394 Size: 429 461 1658 3507 Fragments arranged by size: 3507 1658 461 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2965 4550 4724 65 Size: 1183 1717 1585 174 1396 Fragments arranged by size: 1717 1585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4699 4886 1700 Size: 110 2889 187 2869 Fragments arranged by size: 2889 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 3341 4115 5127 3341 Size: 774 1012 4269 Fragments arranged by size: 4269 1012 774 BstAPI GCAn_nnn'nTGC Cuts at: 794 2086 794 Size: 1292 4763 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 3057 3057 Size: 6055 BstYI r'GATC_y Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050 Size: 1413 367 385 17 1452 86 12 346 Cuts at: 6050 1972 Size: 1977 Fragments arranged by size: 1977 1452 1413 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3069 3069 Size: 6055 BtgI C'CryG_G Cuts at: 513 1065 2065 2254 2907 513 Size: 552 1000 189 653 3661 Fragments arranged by size: 3661 1000 653 552 189 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 2070 2688 3571 4365 4385 2070 Size: 618 883 794 20 3740 Fragments arranged by size: 3740 883 794 618 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DraIII CAC_nnn'GTG Cuts at: 2358 2358 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2121 2811 Size: 54 1425 50 275 18 197 94 690 Cuts at: 2811 2985 3502 4391 6020 8 Size: 174 517 889 1629 43 Fragments arranged by size: 1629 1425 889 690 517 275 197 174 94 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3985 1353 Size: 2632 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2880 4642 5016 5162 387 Size: 1064 138 1291 1762 374 146 1280 Fragments arranged by size: 1762 1291 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 2730 2939 3200 4118 4715 5502 Size: 393 977 121 209 261 918 597 787 Cuts at: 5502 5770 1239 Size: 268 1524 Fragments arranged by size: 1524 977 918 787 597 393 268 261 209 121 EcoO109I rG'GnC_Cy Cuts at: 2083 2134 2569 3073 2083 Size: 51 435 504 5065 Fragments arranged by size: 5065 504 435 51 EcoRI G'AATT_C Cuts at: 3171 3364 3742 3171 Size: 193 378 5484 Fragments arranged by size: 5484 378 193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 6055 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 5202 1678 Size: 3524 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4696 4728 4770 4802 1373 Size: 32 135 32 3124 32 42 32 2626 Fragments arranged by size: 3124 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 2229 3380 2229 Size: 1151 4904 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5169 5353 1305 Size: 3864 184 2007 Fragments arranged by size: 3864 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2029 2813 2987 10 Size: 54 1425 343 197 784 174 3078 Fragments arranged by size: 3078 1425 784 343 197 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 4013 4372 4531 518 Size: 648 951 1896 359 159 2042 Fragments arranged by size: 2042 1896 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2458 2562 3471 4148 5296 5541 1606 Size: 852 104 909 677 1148 245 2120 Fragments arranged by size: 2120 1148 909 852 677 245 104 NcoI C'CATG_G Cuts at: 513 1065 2907 513 Size: 552 1842 3661 Fragments arranged by size: 3661 1842 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 3413 4958 1132 Size: 97 2184 1545 2229 Fragments arranged by size: 2229 2184 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6055 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2134 2083 Size: 51 6004 PshAI GACnn'nnGTC Cuts at: 1613 3003 1613 Size: 1390 4665 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 2568 2568 Size: 6055 PstI C_TGCA'G Cuts at: 830 2601 3407 830 Size: 1771 806 3478 Fragments arranged by size: 3478 1771 806 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2458 2458 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SexAI A'CCwGG_T Cuts at: 2745 3351 2745 Size: 606 5449 SfcI C'TryA_G Cuts at: 826 1045 2597 3305 3403 4538 5219 5410 826 Size: 219 1552 708 98 1135 681 191 1471 Fragments arranged by size: 1552 1471 1135 708 681 219 191 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2879 3323 4159 5060 5322 820 Size: 197 926 936 444 836 901 262 1553 Fragments arranged by size: 1553 936 926 901 836 444 262 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 2310 2699 1517 Size: 793 389 4873 Fragments arranged by size: 4873 793 389 StyI C'CwwG_G Cuts at: 513 1065 2130 2246 2814 2907 2988 3012 3494 Size: 552 1065 116 568 93 81 24 482 Cuts at: 3494 513 Size: 3074 Fragments arranged by size: 3074 1065 568 552 482 116 93 81 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2841 3044 4082 4099 4252 4437 4882 Size: 1255 1261 203 1038 17 153 185 445 Cuts at: 4882 325 Size: 1498 Fragments arranged by size: 1498 1261 1255 1038 445 203 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2055 2436 2805 Size: 274 80 33 51 1443 78 381 369 Cuts at: 2805 4301 6013 96 Size: 1496 1712 138 Fragments arranged by size: 1712 1496 1443 381 369 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6015 6047 6015 Size: 32 6023 Tth111I GACn'n_nGTC Cuts at: 1316 3188 1316 Size: 1872 4183 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2914 2161 Size: 753 5302 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 3089 4184 3089 Size: 1095 4960 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3187 3863 276 Size: 53 83 186 936 1653 676 2468 Fragments arranged by size: 2468 1653 936 676 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI