(Circular) (Six-base) MAPSORT of: pFN21AB6650.seq Check: 3963 from: 1 to: 6043 pFN21AB6650 6043 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 3853 278 Size: 53 83 186 936 582 1735 2468 Fragments arranged by size: 2468 1735 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 1937 Size: 1449 4594 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2501 2687 3075 4106 5490 5758 1632 Size: 869 186 388 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 869 388 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 2120 3324 4942 5919 1203 Size: 917 1204 1618 977 1327 Fragments arranged by size: 1618 1327 1204 977 917 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 2479 3786 5358 2479 Size: 1307 1572 3164 Fragments arranged by size: 3164 1572 1307 ApaLI G'TGCA_C Cuts at: 4100 5256 4100 Size: 1156 4887 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2912 4597 160 Size: 872 1880 1685 1606 Fragments arranged by size: 1880 1685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 2849 3368 1870 Size: 73 906 519 4545 Fragments arranged by size: 4545 906 519 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2476 2509 2476 Size: 33 6010 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3364 4819 618 Size: 325 217 627 1577 1455 1842 Fragments arranged by size: 1842 1577 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2535 3362 1391 Size: 1144 827 4072 Fragments arranged by size: 4072 1144 827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2201 2403 3324 928 Size: 971 302 202 921 3647 Fragments arranged by size: 3647 971 921 302 202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4234 4268 1903 Size: 34 2297 34 3678 Fragments arranged by size: 3678 2297 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3161 3935 5145 3161 Size: 774 1210 4059 Fragments arranged by size: 4059 1210 774 BclI T'GATC_A Cuts at: 1548 3223 1548 Size: 1675 4368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4654 136 Size: 107 122 71 631 605 2982 1525 Fragments arranged by size: 2982 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3285 3309 6038 3285 Size: 24 2729 3290 Fragments arranged by size: 3290 2729 24 BlpI GC'TnA_GC Cuts at: 2529 3460 2529 Size: 931 5112 Bme1580I G_kGCm'C Cuts at: 1790 4104 5260 1790 Size: 2314 1156 2573 Fragments arranged by size: 2573 2314 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3774 4732 442 Size: 416 53 83 399 24 2357 958 1753 Fragments arranged by size: 2357 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2486 4703 1239 Size: 888 359 2217 2579 Fragments arranged by size: 2579 2217 888 359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2706 4168 5033 5331 2706 Size: 1462 865 298 3418 Fragments arranged by size: 3418 1462 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2006 4706 882 Size: 617 507 2700 2219 Fragments arranged by size: 2700 2219 617 507 BsaAI yAC'GTr Cuts at: 493 1204 3325 3834 493 Size: 711 2121 509 2702 Fragments arranged by size: 2702 2121 711 509 BsaBI GATnn'nnATC Cuts at: 2174 2684 6037 2174 Size: 510 3353 2180 Fragments arranged by size: 3353 2180 510 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 3850 4232 4867 Size: 53 83 186 936 582 1735 382 635 Cuts at: 4867 275 Size: 1451 Fragments arranged by size: 1735 1451 936 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4476 4874 5148 5295 5706 4476 Size: 398 274 147 411 4813 Fragments arranged by size: 4813 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2100 2130 1178 Size: 30 165 30 697 30 5091 Fragments arranged by size: 5091 697 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2564 1186 Size: 838 540 4665 Fragments arranged by size: 4665 838 540 BseYI C'CCAG_C Cuts at: 2139 2709 3170 3292 5246 5742 2139 Size: 570 461 122 1954 496 2440 Fragments arranged by size: 2440 1954 570 496 461 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5470 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3493 4254 5282 1540 Size: 275 186 1492 761 1028 2301 Fragments arranged by size: 2301 1492 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2535 4104 4189 5260 2535 Size: 1569 85 1071 3318 Fragments arranged by size: 3318 1569 1071 85 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 3362 4104 4189 5260 1391 Size: 399 745 827 742 85 1071 2174 Fragments arranged by size: 2174 1071 827 745 742 399 85 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 3932 5662 3932 Size: 1730 4313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2304 3398 844 Size: 1460 1094 3489 Fragments arranged by size: 3489 1460 1094 BsrBI CCG'CTC Cuts at: 1394 1823 3178 3930 1394 Size: 429 1355 752 3507 Fragments arranged by size: 3507 1355 752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2898 4538 4712 65 Size: 1183 1650 1640 174 1396 Fragments arranged by size: 1650 1640 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 4874 1700 Size: 110 2877 187 2869 Fragments arranged by size: 2877 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstYI r'GATC_y Cuts at: 1972 2023 3285 3309 3373 3740 4125 4142 5594 Size: 51 1262 24 64 367 385 17 1452 Cuts at: 5594 5680 5692 6038 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1262 385 367 346 86 64 51 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4552 Fragments arranged by size: 4552 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5304 Fragments arranged by size: 5304 683 56 BtsI GCAGTG_nn' Cuts at: 3559 4353 4373 3559 Size: 794 20 5229 Fragments arranged by size: 5229 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3490 4379 6008 Size: 54 1425 50 275 18 1660 889 1629 Cuts at: 6008 8 Size: 43 Fragments arranged by size: 1660 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3490 1537 Size: 275 1678 4090 Fragments arranged by size: 4090 1678 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2427 3014 3973 1353 Size: 1074 587 959 3423 Fragments arranged by size: 3423 1074 959 587 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2533 2533 Size: 6043 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2486 2501 2687 3075 4106 4703 Size: 393 495 359 15 186 388 1031 597 Cuts at: 4703 5490 5758 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1031 787 597 495 393 388 359 268 186 15 EcoNI CCTnn'n_nnAGG Cuts at: 2300 2300 Size: 6043 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2385 3328 2385 Size: 943 5100 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 5190 1678 Size: 3512 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 4684 4716 4758 Size: 32 135 32 528 32 2552 32 42 Cuts at: 4758 4790 1373 Size: 32 2626 Fragments arranged by size: 2626 2552 528 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 3387 3633 669 Size: 988 281 780 669 246 3079 Fragments arranged by size: 3079 988 780 669 281 246 HindIII A'AGCT_T Cuts at: 748 2286 2741 748 Size: 1538 455 4050 Fragments arranged by size: 4050 1538 455 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3265 3642 3265 Size: 377 5666 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 5157 5341 1305 Size: 1260 2592 184 2007 Fragments arranged by size: 2592 2007 1260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4221 Fragments arranged by size: 4221 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 4001 4360 4519 518 Size: 648 1053 1782 359 159 2042 Fragments arranged by size: 2042 1782 1053 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2490 2713 2785 3459 4136 5284 5529 Size: 433 451 223 72 674 677 1148 245 Cuts at: 5529 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 674 451 433 245 223 72 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4552 Fragments arranged by size: 4552 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 2124 2524 3401 4946 1132 Size: 97 895 400 877 1545 2229 Fragments arranged by size: 2229 1545 895 877 400 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6043 PciI A'CATG_T Cuts at: 2120 4942 2120 Size: 2822 3221 PflMI CCAn_nnn'nTGG Cuts at: 1108 3146 1108 Size: 2038 4005 PfoI T'CCnGG_A Cuts at: 3118 3118 Size: 6043 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PmlI CAC'GTG Cuts at: 3325 3325 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PspXI vC'TCGA_Gb Cuts at: 2849 2849 Size: 6043 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 6011 PstI C_TGCA'G Cuts at: 830 2979 3395 830 Size: 2149 416 3478 Fragments arranged by size: 3478 2149 416 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2039 2490 2713 2785 2039 Size: 451 223 72 5297 Fragments arranged by size: 5297 451 223 72 SacI G_AGCT'C Cuts at: 2535 2535 Size: 6043 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SfcI C'TryA_G Cuts at: 826 1045 2564 2975 3297 3391 4526 5207 5398 Size: 219 1519 411 322 94 1135 681 191 Cuts at: 5398 826 Size: 1471 Fragments arranged by size: 1519 1471 1135 681 411 322 219 191 94 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3370 1872 Size: 1498 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2721 2849 4147 5048 5310 820 Size: 197 926 778 128 1298 901 262 1553 Fragments arranged by size: 1553 1298 926 901 778 262 197 128 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6043 StyI C'CwwG_G Cuts at: 513 1065 2004 2301 3231 3482 513 Size: 552 939 297 930 251 3074 Fragments arranged by size: 3074 939 930 552 297 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 4070 4087 4240 4425 4870 325 Size: 1255 573 1917 17 153 185 445 1498 Fragments arranged by size: 1917 1498 1255 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4289 6001 96 Size: 274 80 33 51 1443 2312 1712 138 Fragments arranged by size: 2312 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2676 2708 6003 6035 2676 Size: 32 3295 32 2684 Fragments arranged by size: 3295 2684 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 2849 1943 Size: 906 5137 XmaI C'CCGG_G Cuts at: 1870 3368 1870 Size: 1498 4545 XmnI GAAnn'nnTTC Cuts at: 4172 4172 Size: 6043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 3851 276 Size: 53 83 186 936 582 1735 2468 Fragments arranged by size: 2468 1735 936 582 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI